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ASCP Molecular Biology Certification Exam (Technologist in Molecular Biology, MB(ASCP) board exam prep Complete Q-Bank | 1500 Comprehensive Questions and Verified Answers | 100% Correct

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ASCP Molecular Biology Certification Exam (Technologist in Molecular Biology, MB(ASCP) board exam prep Complete Q-Bank | 1500 Comprehensive Questions and Verified Answers | 100% Correct Quiz_________________? Pyrimidine - Answer☑️ One carbon ring Cytosine, Thymine, Uracil Quiz_________________? What is the function of mRNA? - Answer☑️ Carries genetic info out of nucleus Transcript translated to protein Quiz_________________? What is the function of tRNA? - Answer☑️ Carries aa to ribosome Anticodon pairs with codon on mRNA strand Quiz_________________? What is the function of rRNA? - Answer☑️ part of ribosome structure most abundant RNA coordinated coupling of tRNA to mRNA codons Quiz_________________? Feedback inhibition - Answer☑️ Product of pathway is noncompetitive inhibitor Binds to allosteric site to slow down rxn b/c too much product Quiz_________________? Endonucleases (Prok) - Answer☑️ Restriction enzymes Cleaves phoshpodiester bonds w/i poly-nt chain Recognition site is palindromic sequence Types I-V Quiz_________________? ORI sites - Answer☑️ nt sequence where replication is initiated Quiz_________________? Topoisomerase I - Answer☑️ Induces ss breaks Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination; for chr condensation; and to disentangle intertwined DNA during mitosis Quiz_________________? topoisomerase II - Answer☑️ cuts both strands of one DNA double helix, passes another unbroken DNA helix through it, and then reanneals the cut strands Quiz_________________? Gyrase (topoisomerase II) - Answer☑️ Unwinds supercoiling caused by unwinding at the rep fork by introducing DSBs Quiz_________________? Helicase - Answer☑️ Breaks H-bonds of double helix at the replication fork Quiz_________________? single-strand DNA binding proteins (SSBPs) - Answer☑️ Binds ssDNA and prevents it from re-annealing during TXN, replication, repair, and recombination Quiz_________________? Telomeres - Answer☑️ Repeat sequence (TTAGGG) at the ends of chr, protect chr from degradation Quiz_________________? RNA polymerase - Answer☑️ DNA dependent RNApol Transcribes DNA template to RNA (3'--5'; anti-parallel) Quiz_________________? Splicesomes - Answer☑️ Complex of snRNPs Removes introns from pre-mRNA and splices exons together Quiz_________________? Enhancers - Answer☑️ Short regions of DNA that bind proteins (TXN factors) that enhance TXN of a gene Quiz_________________? Reverse transcriptase - Answer☑️ enzyme that transcribes RNA to cDNA (lacks introns) RNA -- RNA:DNA -- cDNA (dsDNA) Quiz_________________? Open Reading Frame (ORF) - Answer☑️ sections of DNA that begin with start codons and end with stop codons DNA: 5' -- 3' transcription: 3' -- 5' DNA -- RNA (promoter)

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1




Quiz_________________?
Pyrimidine -

Answer☑️

One carbon ring
Cytosine, Thymine, Uracil


Quiz_________________?
What is the function of mRNA? -

Answer☑️
Carries genetic info out of nucleus
Transcript translated to protein


Quiz_________________?
What is the function of tRNA? -

Answer☑️
Carries aa to ribosome
Anticodon pairs with codon on mRNA strand

, Quiz_________________?
2
What is the function of rRNA? -

Answer☑️

part of ribosome structure
most abundant RNA
coordinated coupling of tRNA to mRNA codons


Quiz_________________?
Feedback inhibition -

Answer☑️

Product of pathway is noncompetitive inhibitor
Binds to allosteric site to slow down rxn b/c too much product


Quiz_________________?
Endonucleases (Prok) -

Answer☑️

Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Recognition site is palindromic sequence
Types I-V


Quiz_________________?
ORI sites -

Answer☑️

nt sequence where replication is initiated


Quiz_________________?
Topoisomerase I -

Answer☑️

, Induces ss breaks
3
Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination;
for chr condensation; and to disentangle intertwined DNA during mitosis


Quiz_________________?
topoisomerase II -

Answer☑️

cuts both strands of one DNA double helix, passes another unbroken DNA helix through it, and then
reanneals the cut strands


Quiz_________________?
Gyrase (topoisomerase II) -

Answer☑️

Unwinds supercoiling caused by unwinding at the rep fork by introducing DSBs


Quiz_________________?
Helicase -

Answer☑️
Breaks H-bonds of double helix at the replication fork


Quiz_________________?
single-strand DNA binding proteins (SSBPs) -

Answer☑️

Binds ssDNA and prevents it from re-annealing during TXN, replication, repair, and recombination


Quiz_________________?
Telomeres -

Answer☑️

Repeat sequence (TTAGGG) at the ends of chr, protect chr from degradation

, Quiz_________________?
4
RNA polymerase -

Answer☑️

DNA dependent RNApol
Transcribes DNA template to RNA (3'-->5'; anti-parallel)


Quiz_________________?
Splicesomes -

Answer☑️
Complex of snRNPs
Removes introns from pre-mRNA and splices exons together


Quiz_________________?
Enhancers -

Answer☑️

Short regions of DNA that bind proteins (TXN factors) that enhance TXN of a gene


Quiz_________________?
Reverse transcriptase -

Answer☑️

enzyme that transcribes RNA to cDNA (lacks introns)
RNA --> RNA:DNA --> cDNA (dsDNA)


Quiz_________________?
Open Reading Frame (ORF) -

Answer☑️
sections of DNA that begin with start codons and end with stop codons
DNA: 5' --> 3'
transcription: 3' --> 5' DNA --> RNA (promoter)

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