The Electrically Conductive Pili of Geobacter Species are a Recently Evolved Feature for Extracellular Electron Transfer

Supplementary Table S2. Comparison of amino acid sequences from pilin accessory proteins found in the genomes of e-pilin
harboring organisms to accessory proteins from such well-characterized species as Neisseria gonorrhoeae, Escherichia coli,
Pseudomonas aeruginosa, and Myxococcus fulvus. Percent identity and percent similarity values were generated with the BLASTp
algorithm. If query coverage was lower than 65%, similarity values were not included in the table and these cells were given a grey
background. NA indicates that a homologous gene was not detected in the specified genome.

Geobacter Locus ID % iden/sim % iden/sim % iden/sim % iden/sim
sulfurreducens N. gonorrhoeae E. coli P. aeruginosa M. fulvus
pilV GSU1063 NA
pilW GSU1064 NA NA
pilX GSU1065 NA
pilY1 GSU1066 NA
pilB GSU1491 44/64 46/66 45/66 65/83
pilT GSU1492 48/67 51/67 50/69
pilC GSU1493 30/53 41/62 60/76
pilS GSU1494 27/43 35/53
pilR GSU1495 43/62 48/65
pilQ GSU2028 33/49 35/52
pilP GSU2029
pilO GSU2030 26/48 33/56
pilN GSU2031 22/43
pilM GSU2032 26/46 52/72
pilD GSU2043 36/55 34/53 41/58 41/62


Pelobacter Locus ID % iden/sim % iden/sim % iden/sim % iden/sim
carbinolicus N. gonorrhoeae E. coli P. aeruginosa M. fulvus
pilV Pcar_2158 NA
pilW Pcar_2157 NA 37/66 NA
pilX Pcar_2156 25/39 NA
pilY1 Pcar_2155 NA
pilB Pcar_2149 42/64 43/64 45/66 65/83
pilT Pcar_2148 46/63 51/67 49/67
pilC Pcar_2147 28/48 44/63 61/75

,pilS Pcar_2146 27/45 34/53
pilR Pcar_2145 42/61 46/62
pilQ Pcar_2134 32/48 30/46
pilP Pcar_2135 37/52
pilO Pcar_2136 24/51 32/55
pilN Pcar_2137 26/48
pilM Pcar_2138 25/44 47/68
pilD Pcar_2139 37/54 33/52 35/55 38/62




Geobacter Locus ID % iden/sim % iden/sim % iden/sim % iden/sim
metallireducens N. gonorrhoeae E. coli P. aeruginosa M. fulvus
pilV Gmet_0734 NA
pilW Gmet_0735 NA NA
pilX Gmet_0736 NA
pilY1 Gmet_0737 NA
pilB Gmet_1393 44/65 46/65 45/66 65/83
pilT Gmet_1394 48/67 51/68 49/68
pilC Gmet_1395 30/52 41/62 62/77
pilS Gmet_1396 27/45 36/53
pilR Gmet_1397 44/62 49/66
pilQ Gmet_0975 31/49 35/52
pilP Gmet_0974
pilO Gmet_0973 30/50 31/52
pilN Gmet_0972 25/45
pilM Gmet_0971 25/44 51/70
pilD Gmet_0959 37/54 33/51 40/55 39/61




Desulfobacterium Locus ID % iden/sim % iden/sim % iden/sim % iden/sim
autotrophicum N. gonorrhoeae E. coli P. aeruginosa M. fulvus

, pilV HRM2_27670 31/69 NA
pilW HRM2_27680 28/62 NA NA
pilX HRM2_27660 17/34 NA
pilY1 HRM2_27650 NA 23/34 23/35
pilB HRM2_23080 40/61 42/60 40/60 46/64
pilC HRM2_22330 28/49 40/61 55/70
pilT HRM2_04990 52/72 58/74 46/65
pilS HRM2_10980
pilR HRM2_10970 39/58 42/58
pilQ HRM2_11030 32/51
pilP HRM2_11020 28/41
pilO HRM2_11010 30/50 30/52
pilN HRM2_11000 27/50 28/53
PilM HRM2_10990 22/41 33/57
pilD HRM2_30410 34/50 33/49 34/49 36/57



Desulfobotulus Locus ID % iden/sim % iden/sim % iden/sim % iden/sim
sapovorans N. gonorrhoeae E. coli P. aeruginosa M. fulvus
pilV BR04DRAFT_02178 NA
pilW BR04DRAFT_02177 NA NA
pilX BR04DRAFT_02176 NA
pilY1 BR04DRAFT_02174 NA
pilB BR04DRAFT_02193 37/57 34/52 38/55 NA
pilT BR04DRAFT_00210 53/72 52/75 48/64
pilC BR04DRAFT_02614
pilS BR04DRAFT_01794
pilR BR04DRAFT_01795 43/60 42/59
pilQ BR04DRAFT_01789 33/49
pilP BR04DRAFT_01790
pilO BR04DRAFT_01791 19/39 29/48
pilN BR04DRAFT_01792 23/42
pilM BR04DRAFT_01793 21/43 40/60