ASCP BLOOD BANK PRACTICE
QUESTIONS WITH CORRECT
ANSWERS!!
Results of a serum sample tested against a panel of reagent red cells gives
presumptive evidence of an alloantibody directed against a high incidence antigen.
Further investigation to confirm the specificity should include which of the following?
A.serum testing against red cells from random donors
B.serum testing against red cells known to lack high incidence antigens
C.serum testing against enzyme treated autologous red cells
D.testing of an eluate prepared from the patient's red cells - ANSWERB. serum
testing against red cells known to lack high incidence antigens
Fetal neonatal alloimmune thrombocytopenia (FNAIT) is caused by which of the
following maternal antibodies?
A.IgM alloantibodies against ABO antigens
B.IgG alloantibodies against HLA antigens
C.IgM alloantibodies against glycoprotein V (GPV) antigens
D.IgG alloantibodies against HPA antigens - ANSWERD. IgG alloantibodies against
HPA antigens
FNAIT is due to maternal IgG alloantibodies crossing the placenta and reacting with
fetal platelet HPA antigens.
The purpose of testing with anti-A,B is to detect:
A.anti-A1
B.anti-A2
C.subgroups of A
D.subgroups of O - ANSWERSubgroups of A
Ax cells react more strongly with anti-A,B than with anti-A. If anti-A is nonreactive, Ax
cells may be detected with anti-A,B.
What increment of platelets in the typical 70-kg human is expected to result from
each single unit of Platelets transfused to a non-HLA-sensitized recipient?
A.3,000 - 5,000/µL
B.5,000 - 10,000/µL
C.20,000 - 25,000/µL
D.25,000 - 30,000/µL - ANSWERB.5,000 - 10,000/µL
One unit of Platelets made from whole blood should cause the platelet count to
increase from 5,000-10,000/µL.
All donor blood testing must include:
A.complete Rh phenotyping
, B.anti-CMV testing
C.direct antiglobulin test
D.serological test for syphilis - ANSWERD.serological test for syphilis
A sample gives the following results:
Cells with Anti A: 3+ Anti B: 4+
Serum with A1: 2+ and B: 0
Which lectin should be used first to resolve this discrepancy?
A.Ulex europaeus
B.Arachis hypogaea
C.Dolichos biflorus
D.Vicia graminea - ANSWERC.Dolichos biflorus
Dolichos biflorus plant seed extract forms complexes with N-acetylgalactosamine.
When properly diluted, it can distinguish between A1 donor cells and all other
subgroups of A.
Transfusion-associated HTLV I/II incidence is low due to the following laboratory
testing:
A.nucleic acid testing for HTLV I/II
B.HTLV I core antibody testing by ELISA
C.HTLV II surface antigen testing by chemiluminescent immunoassay (ChLIA)
D.IgG antibody testing for HTLV I/II by ELISA or ChLIA - ANSWERD.IgG antibody
testing for HTLV I/II by ELISA or ChLIA
A patient demonstrates 4+ reactivity with all red cells tested and the autocontrol is
nonreactive. This high incidence antibody was suspected to be related to the P1PK
blood group system as the patient is the rare p phenotype. What antibody specificity
should be suspected?
A.anti-IP1
B.anti-P2
C.anti-PP1Pk
D.anti-P1 - ANSWERC.anti-PP1Pk
Anti-PP1Pk is the high incidence antibody made by individuals with the p phenotype.
This antibody has an association with early spontaneous abortion.
Which unit should be selected for exchange transfusion if the newborn is group A,
Rh-positive and the mother is group A, Rh-positive with anti-c?
A.A, CDe/CDe
B.A, cDE/cDE
C.O, cde/cde
D.A, cde/cde - ANSWERA.A, CDe/CDe
In suspected cases of hemolytic disease of the fetus and newborn, what significant
information can be obtained from the baby's blood smear?
A.estimation of WBC, RBC, and platelet counts
B.marked increase in immature neutrophils (shift to the left)
QUESTIONS WITH CORRECT
ANSWERS!!
Results of a serum sample tested against a panel of reagent red cells gives
presumptive evidence of an alloantibody directed against a high incidence antigen.
Further investigation to confirm the specificity should include which of the following?
A.serum testing against red cells from random donors
B.serum testing against red cells known to lack high incidence antigens
C.serum testing against enzyme treated autologous red cells
D.testing of an eluate prepared from the patient's red cells - ANSWERB. serum
testing against red cells known to lack high incidence antigens
Fetal neonatal alloimmune thrombocytopenia (FNAIT) is caused by which of the
following maternal antibodies?
A.IgM alloantibodies against ABO antigens
B.IgG alloantibodies against HLA antigens
C.IgM alloantibodies against glycoprotein V (GPV) antigens
D.IgG alloantibodies against HPA antigens - ANSWERD. IgG alloantibodies against
HPA antigens
FNAIT is due to maternal IgG alloantibodies crossing the placenta and reacting with
fetal platelet HPA antigens.
The purpose of testing with anti-A,B is to detect:
A.anti-A1
B.anti-A2
C.subgroups of A
D.subgroups of O - ANSWERSubgroups of A
Ax cells react more strongly with anti-A,B than with anti-A. If anti-A is nonreactive, Ax
cells may be detected with anti-A,B.
What increment of platelets in the typical 70-kg human is expected to result from
each single unit of Platelets transfused to a non-HLA-sensitized recipient?
A.3,000 - 5,000/µL
B.5,000 - 10,000/µL
C.20,000 - 25,000/µL
D.25,000 - 30,000/µL - ANSWERB.5,000 - 10,000/µL
One unit of Platelets made from whole blood should cause the platelet count to
increase from 5,000-10,000/µL.
All donor blood testing must include:
A.complete Rh phenotyping
, B.anti-CMV testing
C.direct antiglobulin test
D.serological test for syphilis - ANSWERD.serological test for syphilis
A sample gives the following results:
Cells with Anti A: 3+ Anti B: 4+
Serum with A1: 2+ and B: 0
Which lectin should be used first to resolve this discrepancy?
A.Ulex europaeus
B.Arachis hypogaea
C.Dolichos biflorus
D.Vicia graminea - ANSWERC.Dolichos biflorus
Dolichos biflorus plant seed extract forms complexes with N-acetylgalactosamine.
When properly diluted, it can distinguish between A1 donor cells and all other
subgroups of A.
Transfusion-associated HTLV I/II incidence is low due to the following laboratory
testing:
A.nucleic acid testing for HTLV I/II
B.HTLV I core antibody testing by ELISA
C.HTLV II surface antigen testing by chemiluminescent immunoassay (ChLIA)
D.IgG antibody testing for HTLV I/II by ELISA or ChLIA - ANSWERD.IgG antibody
testing for HTLV I/II by ELISA or ChLIA
A patient demonstrates 4+ reactivity with all red cells tested and the autocontrol is
nonreactive. This high incidence antibody was suspected to be related to the P1PK
blood group system as the patient is the rare p phenotype. What antibody specificity
should be suspected?
A.anti-IP1
B.anti-P2
C.anti-PP1Pk
D.anti-P1 - ANSWERC.anti-PP1Pk
Anti-PP1Pk is the high incidence antibody made by individuals with the p phenotype.
This antibody has an association with early spontaneous abortion.
Which unit should be selected for exchange transfusion if the newborn is group A,
Rh-positive and the mother is group A, Rh-positive with anti-c?
A.A, CDe/CDe
B.A, cDE/cDE
C.O, cde/cde
D.A, cde/cde - ANSWERA.A, CDe/CDe
In suspected cases of hemolytic disease of the fetus and newborn, what significant
information can be obtained from the baby's blood smear?
A.estimation of WBC, RBC, and platelet counts
B.marked increase in immature neutrophils (shift to the left)
