PAPER CHROMATOGRAPHY LAB
REVIEW UPDATED ACTUAL Exam
Questions and CORRECT Answers
All 7 dyes have - CORRECT ANSWER - Conjugated systems
Conjugated systems - CORRECT ANSWER - Long carbon structures with alternating
carbon-carbon single and double bonds
Lots of conjugated bonds means - CORRECT ANSWER - Color
Chromatography - CORRECT ANSWER - Group of techniques used to separate colored
mixtures into their component parts (often used for blood or drinking water analyses)
Chromatography involves - CORRECT ANSWER - A stationary phase and a mobile
phase
Stationary phase can be - CORRECT ANSWER - Solid or liquid
The mixture to be separated is placed - CORRECT ANSWER - On the stationary phase
Mobile phase can be - CORRECT ANSWER - Liquid or gas
Liquid chromatography - CORRECT ANSWER - The separation is based on preferential
attraction of each component of the mixture to either stationary or mobile phase
The mobile phase moves along the stationary phase, - CORRECT ANSWER - Carrying
some or all of the mixture with it, resulting in the separation of the mixture components
, Attractions of components to mobile or stationary phase is due to - CORRECT
ANSWER - Intermolecular interactions
Paper chromatography - CORRECT ANSWER - Sample of mixture is put on
chromatography paper, which acts as stationary phase; edge of paper placed in solvent, which
acts as mobile phase
Wicking occurs because - CORRECT ANSWER - Solvent is attracted to water molecules
that are bound to cellulose fibers of paper
In paper chromatography, the sample is - CORRECT ANSWER - Spotted on the origin
line
Once sample has been spotted, - CORRECT ANSWER - Bottom edge of paper is put in
solvent and solvent moves up paper; when solvent front reaches sample, the sample components
are attracted to either stationary or mobile phase
Solvent front - CORRECT ANSWER - Leading edge of mobile phase
Attraction depends on what property? - CORRECT ANSWER - Relative polarities of
sample components and two phases
The attraction is never - CORRECT ANSWER - All or nothing
Equilibrium equation - CORRECT ANSWER - Component-mobile phase< -->
component-stationary phase
As fresh solvent continually passes the sample, - CORRECT ANSWER - New equilibria
are established
REVIEW UPDATED ACTUAL Exam
Questions and CORRECT Answers
All 7 dyes have - CORRECT ANSWER - Conjugated systems
Conjugated systems - CORRECT ANSWER - Long carbon structures with alternating
carbon-carbon single and double bonds
Lots of conjugated bonds means - CORRECT ANSWER - Color
Chromatography - CORRECT ANSWER - Group of techniques used to separate colored
mixtures into their component parts (often used for blood or drinking water analyses)
Chromatography involves - CORRECT ANSWER - A stationary phase and a mobile
phase
Stationary phase can be - CORRECT ANSWER - Solid or liquid
The mixture to be separated is placed - CORRECT ANSWER - On the stationary phase
Mobile phase can be - CORRECT ANSWER - Liquid or gas
Liquid chromatography - CORRECT ANSWER - The separation is based on preferential
attraction of each component of the mixture to either stationary or mobile phase
The mobile phase moves along the stationary phase, - CORRECT ANSWER - Carrying
some or all of the mixture with it, resulting in the separation of the mixture components
, Attractions of components to mobile or stationary phase is due to - CORRECT
ANSWER - Intermolecular interactions
Paper chromatography - CORRECT ANSWER - Sample of mixture is put on
chromatography paper, which acts as stationary phase; edge of paper placed in solvent, which
acts as mobile phase
Wicking occurs because - CORRECT ANSWER - Solvent is attracted to water molecules
that are bound to cellulose fibers of paper
In paper chromatography, the sample is - CORRECT ANSWER - Spotted on the origin
line
Once sample has been spotted, - CORRECT ANSWER - Bottom edge of paper is put in
solvent and solvent moves up paper; when solvent front reaches sample, the sample components
are attracted to either stationary or mobile phase
Solvent front - CORRECT ANSWER - Leading edge of mobile phase
Attraction depends on what property? - CORRECT ANSWER - Relative polarities of
sample components and two phases
The attraction is never - CORRECT ANSWER - All or nothing
Equilibrium equation - CORRECT ANSWER - Component-mobile phase< -->
component-stationary phase
As fresh solvent continually passes the sample, - CORRECT ANSWER - New equilibria
are established
