: Phlebotomy Tubes and Additives – Order of Draw and Test Applications

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: Phlebotomy Tubes and Additives – Order of Draw and Test Applications

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2025/2026

Institution Phlebotomy

Course Phlebotomy

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This document summarizes phlebotomy tubes, their additives, and their specific laboratory uses. It covers the order of draw, tube colors, anticoagulants such as EDTA, heparin, sodium citrate, and sodium fluoride, as well as serum separator tubes and specialized tubes like royal blue and tan. It also explains which tests are performed with each tube, including chemistry panels, coagulation studies, hematology, blood cultures, and toxicology.

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Phlebotomy tubes and additives

blood culture - ANS -contains SPS, yellow top
- draw first because you need to use sterile technique

clot activator - ANS -Coagulation enhancing substance, such as silica (glass)

EDTA - ANS -binds Calcium to prevent coagulation

gold and red/gray top tubes - ANS -a serum separator gel and no additives
(maybe a clot activator, SST)
-chemistry, immunology
-draw fourth after red top tube

Gray tube - ANS Glucose Tolerance Test, Fasting Blood Sugar, Lactic Acid,
Blood alcohol
- potassium oxalate and sodium fluoride

Green, green/gray top tube - ANS -Additive: sodium heparin, lithium heparin,
ammonium heparin
- chemistry
- can have a gel separator (light, PST)
-draw fifth after gold or red/gray tubes

Heparin - ANS - sodium, lithium, ammonium
- inhibits thrombin from forming

Lavender Tube (pink or pearl) - ANS -Contains EDTA. Used in Hematology &
blood bank
-tests CBC, WBC, RBC, Platelet counts, any blood counts
-draw sixth after green tubes

Light Blue Tube - ANS -Contains Sodium Citrate.
-Used in Coagulation dept.
-Tests for PT, PTT, Fibrinogen, clotting factors
-draw second after blood culture

Orange tube used for: - ANS STAT serum chemistries
- contains thrombin

Order of draw - ANS 1. Yellow
2. Light Blue

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Uploaded on: September 11, 2025
Number of pages: 2
Written in: 2025/2026
Type: Exam (elaborations)
Contains: Questions & answers

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