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MLT ASCP
MLT ASCP Exam PREP| Questions &
Answers | Grade A | 100% Correct |
(NEW 2025/ 2026 Update)
1. A class ELISA test is performed on a sample from a 67-year-old male
patient who is suspected of having ingested large amounts of drug
X. The ELISA procedure us performed as follows:
1. Incubation of patient sample in antibody-coated microtiter plate
wells.
2. Plate washing
3. Addition of peroxidase-linked drug X conjugate
4. Incubation followed by plate washing
5. Addition of hydrogen peroxide
6. Evaluation of color change
MLT ASCP
,Which of the following is true regarding this procedure?: hydrogen
peroxide is the substrate
2. Enzyme-linked immunosorbent assay (ELISA) technique for the
detection of hbsag: uses anti-hbs linked to horseradish peroxidase
3. Igm antibodies are frequently hemolytic because of: their
efficient ability to fix complement
4. Which of the figures demonstrates a reaction pattern of partial
identity?: figure 2
5. Substances that are antigenic only when coupled to a protein
carrier are:: haptens
6. Which of the following collection types should be performed to
obtain a highly concentrated urine specimen?: first morning
7. Which of the following immunoglobulins is the most efficient at
agglutination: igm
8. In an indirect ELISA method designed to detect antibody to the
rubella virus in patient serum, the conjugate used should be.: anti-
human igg conjugated to an enzyme
,9. A deficiency to arginine vasopressin ( antidiuretic hormone
ADH) is associated with: hyposthenuria
10. The presence of small, dark, pepper-like granules in a
synovial fluid is strongly associated with: alkaptonuria
11. Nonspecifically killing tumor cells is carried out by: natural
killer cells
12. Ascites is collected by: paracentesis
13. A vaginal swab is received in the laboratory for testing
immediately placed in sterile physiological saline to prepare a
suspension. A wet mount is prepared, and the laboratorian reports
the presence of numerous clue cells. This most likely indicated the
patient has:: bacterial vaginosis
14. Delayed hypersensitivity may be induced by: contact
sensitivity to inorganic chemicals
15. Which of the following analyses is performed on seminal
fluid that has been fixed and stained with Wright, Giemsa, or
Papanicolaou stain: morphology
, 16. A physician wants to determine if an abdominal fluid
collected during surgery, is urine. Which of the following analytes
would normally be present in the urine of an apparently healthy
patient?: creatine
17. Seminal fluid diluted 1:20 and loaded onto a Neubauer
counting chamber. An average of 5 sperm are counted in 5
secondary squares. Sperm concentration should be reported as.:
5,000,000/ml
18. Membrane-bound immunoglobulin molecules:: are
anchored by a hydrophobic sequence of about 26 residues
19. The most commonly used serological indicator of recent
streptococcal infection is the antibody to:: Streptolysin O
20. From the test results in the table, it can be concluded that
patient #3 has: Patient hbsag anti-hbs igm anti-HAV igm
1--+
2++-
3 - + -: acute hepatitis B
MLT ASCP
MLT ASCP Exam PREP| Questions &
Answers | Grade A | 100% Correct |
(NEW 2025/ 2026 Update)
1. A class ELISA test is performed on a sample from a 67-year-old male
patient who is suspected of having ingested large amounts of drug
X. The ELISA procedure us performed as follows:
1. Incubation of patient sample in antibody-coated microtiter plate
wells.
2. Plate washing
3. Addition of peroxidase-linked drug X conjugate
4. Incubation followed by plate washing
5. Addition of hydrogen peroxide
6. Evaluation of color change
MLT ASCP
,Which of the following is true regarding this procedure?: hydrogen
peroxide is the substrate
2. Enzyme-linked immunosorbent assay (ELISA) technique for the
detection of hbsag: uses anti-hbs linked to horseradish peroxidase
3. Igm antibodies are frequently hemolytic because of: their
efficient ability to fix complement
4. Which of the figures demonstrates a reaction pattern of partial
identity?: figure 2
5. Substances that are antigenic only when coupled to a protein
carrier are:: haptens
6. Which of the following collection types should be performed to
obtain a highly concentrated urine specimen?: first morning
7. Which of the following immunoglobulins is the most efficient at
agglutination: igm
8. In an indirect ELISA method designed to detect antibody to the
rubella virus in patient serum, the conjugate used should be.: anti-
human igg conjugated to an enzyme
,9. A deficiency to arginine vasopressin ( antidiuretic hormone
ADH) is associated with: hyposthenuria
10. The presence of small, dark, pepper-like granules in a
synovial fluid is strongly associated with: alkaptonuria
11. Nonspecifically killing tumor cells is carried out by: natural
killer cells
12. Ascites is collected by: paracentesis
13. A vaginal swab is received in the laboratory for testing
immediately placed in sterile physiological saline to prepare a
suspension. A wet mount is prepared, and the laboratorian reports
the presence of numerous clue cells. This most likely indicated the
patient has:: bacterial vaginosis
14. Delayed hypersensitivity may be induced by: contact
sensitivity to inorganic chemicals
15. Which of the following analyses is performed on seminal
fluid that has been fixed and stained with Wright, Giemsa, or
Papanicolaou stain: morphology
, 16. A physician wants to determine if an abdominal fluid
collected during surgery, is urine. Which of the following analytes
would normally be present in the urine of an apparently healthy
patient?: creatine
17. Seminal fluid diluted 1:20 and loaded onto a Neubauer
counting chamber. An average of 5 sperm are counted in 5
secondary squares. Sperm concentration should be reported as.:
5,000,000/ml
18. Membrane-bound immunoglobulin molecules:: are
anchored by a hydrophobic sequence of about 26 residues
19. The most commonly used serological indicator of recent
streptococcal infection is the antibody to:: Streptolysin O
20. From the test results in the table, it can be concluded that
patient #3 has: Patient hbsag anti-hbs igm anti-HAV igm
1--+
2++-
3 - + -: acute hepatitis B
