MB (ASCP) Exam 2025 update| comprehensive
questions and verified answers (complete solutions)
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Predominant form of DNA - (ANSWER)Right-handed B
DNA and histone interaction is due to what type of bond? - (ANSWER)Ionic
What stains to the minor groove of DNA duplex? - (ANSWER)SYBR Green
What is the most abundant type of RNA? - (ANSWER)Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - (ANSWER)Genome and mRNA
What increases half-life of mRNA? - (ANSWER)5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -
(ANSWER)Euchromatin
What is the most common type of eukaryotic DNA? - (ANSWER)Satellite DNA
What specimen has the most DNA according to molecular weight? -
(ANSWER)White Blood Cells
,What amino acids compose histones? - (ANSWER)Lysine and Arginine
Amino acid that all proteins start with: - (ANSWER)Methionine
What protects the DNA strand during replication? - (ANSWER)Single-stranded
binding protein
Leading Strand - (ANSWER)3' to 5' template
Lagging Strand - (ANSWER)5' to 3' template
What DNA strand is being copied in transcription? - (ANSWER)Antisense Strand
First step after newly formed mRNA is made? - (ANSWER)Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme? - (ANSWER)Telomerase
What polymerase has repair activity? - (ANSWER)Beta/Epsilon
What polymerase acts on leading strand? - (ANSWER)Delta
, What polymerase acts on lagging strand? - (ANSWER)Alpha
Which polymerase is used for mitochondrial replication and repair? -
(ANSWER)Gamma
What polymerase binds the 5' end to 3' end of dsDNA? -
(ANSWER)Topoisomerase
Which RNA polymerase is mainly for transcription? - (ANSWER)RNA Pol II
Which polymerase is involved with mRNA synthesis? - (ANSWER)RNA Pol II
What inactivates RNAses? - (ANSWER)Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -
(ANSWER)Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? -
(ANSWER)Restriction Enzyme Type II
What is necessary to make cDNA? - (ANSWER)Reverse transcriptase
questions and verified answers (complete solutions)
ASSURED SUCCESS|GRADE A+!! (100% ACCURATE)
GET IT RIGHT!!
Predominant form of DNA - (ANSWER)Right-handed B
DNA and histone interaction is due to what type of bond? - (ANSWER)Ionic
What stains to the minor groove of DNA duplex? - (ANSWER)SYBR Green
What is the most abundant type of RNA? - (ANSWER)Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - (ANSWER)Genome and mRNA
What increases half-life of mRNA? - (ANSWER)5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -
(ANSWER)Euchromatin
What is the most common type of eukaryotic DNA? - (ANSWER)Satellite DNA
What specimen has the most DNA according to molecular weight? -
(ANSWER)White Blood Cells
,What amino acids compose histones? - (ANSWER)Lysine and Arginine
Amino acid that all proteins start with: - (ANSWER)Methionine
What protects the DNA strand during replication? - (ANSWER)Single-stranded
binding protein
Leading Strand - (ANSWER)3' to 5' template
Lagging Strand - (ANSWER)5' to 3' template
What DNA strand is being copied in transcription? - (ANSWER)Antisense Strand
First step after newly formed mRNA is made? - (ANSWER)Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme? - (ANSWER)Telomerase
What polymerase has repair activity? - (ANSWER)Beta/Epsilon
What polymerase acts on leading strand? - (ANSWER)Delta
, What polymerase acts on lagging strand? - (ANSWER)Alpha
Which polymerase is used for mitochondrial replication and repair? -
(ANSWER)Gamma
What polymerase binds the 5' end to 3' end of dsDNA? -
(ANSWER)Topoisomerase
Which RNA polymerase is mainly for transcription? - (ANSWER)RNA Pol II
Which polymerase is involved with mRNA synthesis? - (ANSWER)RNA Pol II
What inactivates RNAses? - (ANSWER)Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -
(ANSWER)Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? -
(ANSWER)Restriction Enzyme Type II
What is necessary to make cDNA? - (ANSWER)Reverse transcriptase
