Certification Exam Practice Questions
And Correct Answers (Verified Answers)
Plus Rationales 2025|2026 Q&A |
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1. What is the first step a phlebotomy technician should take before
drawing blood?
Verify the patient’s identity.
Rationale: Correct patient identification is critical to prevent errors
and ensure patient safety.
2. Which vein is most commonly used for venipuncture?
Median cubital vein.
Rationale: It is large, easily accessible, and less likely to be near
arteries or nerves.
,3. What should a phlebotomist do if a patient refuses a blood draw?
Document the refusal and notify the supervisor.
Rationale: Patient autonomy must be respected, and proper
documentation protects both patient and healthcare staff.
4. How long should a tourniquet remain on a patient’s arm?
No longer than one minute.
Rationale: Prolonged application can cause hemoconcentration and
affect test results.
5. What is the primary purpose of using gloves during a blood draw?
To protect both the patient and phlebotomist from infection.
Rationale: Gloves act as a barrier to prevent exposure to bloodborne
pathogens.
6. What is the correct order of draw for multiple tube collections?
Blood culture, light blue, red, gold, green, lavender, gray.
Rationale: Following the correct order prevents cross-contamination
and additive interference.
7. How should a phlebotomist handle a patient who faints during a blood
draw?
Stop the procedure, remove the needle, and assist the patient to a
safe position.
Rationale: Immediate safety is a priority, and proper positioning
prevents injury.
,8. What is hemolysis?
The rupture of red blood cells.
Rationale: Hemolysis can alter lab results, especially potassium and
hematology tests.
9. What is the standard antiseptic used for routine venipuncture?
70% isopropyl alcohol.
Rationale: It effectively reduces the risk of introducing pathogens
into the bloodstream.
10. Why is patient hydration important before drawing blood?
It improves vein visibility and makes venipuncture easier.
Rationale: Proper hydration expands veins, reducing the likelihood of
complications.
11. Which of the following can affect test results if the patient has
not fasted?
Blood glucose levels.
Rationale: Eating before a test can increase glucose and lipid levels,
affecting results.
12. What is the proper way to label a blood specimen?
Immediately after collection with patient’s name, date, and time.
Rationale: Labeling at the bedside prevents misidentification and
ensures specimen integrity.
, 13. What should be done if a specimen is hemolyzed?
Repeat the blood draw if necessary and notify the laboratory.
Rationale: Hemolyzed specimens can give inaccurate results.
14. What is the appropriate needle gauge for routine venipuncture
in adults?
21-gauge needle.
Rationale: A 21-gauge needle balances patient comfort with
adequate blood flow.
15. What type of infection control precautions are required for all
patients?
Standard precautions.
Rationale: Standard precautions assume all blood and body fluids
may be infectious.
16. How should a phlebotomist dispose of a used needle?
Immediately in a sharps container without recapping.
Rationale: Prevents needlestick injuries and reduces risk of infection.
17. What is the maximum time a blood culture bottle should sit at
room temperature before incubation?
No more than two hours.
Rationale: Delays can allow bacterial growth to alter test results.
18. What is the term for blood collected from a vein?
Venous blood.