Lab Exam Correct Questions With
Solved Answers
What pipette and what setting would you use to get 1000 microliters (1 mL)? Solved
Answers p1000 and 100.
What pipette and what setting would you use to get 50 microliters (0.05 mL)? Solved
Answers p 200 and 050.
How to get the final percentage of each ingredient? Solved Answers Take the ingredient
total divided by the overall total.
What are the three kinds of agar plates used for in Week 5? Solved Answers Bacterial
growth.
What is the 50x TAE buffer used for in week 4? Solved Answers To make DNA gel.
What is a plasmid? Solved Answers A piece of DNA that can exist outside the cell's
chromosome.
What are the three buffers in Week 3, and what are their functions? Solved Answers
MX1: contains RNAse (enzyme degrading RNA)
MX2: contains detergent that disrupts bacterial cell membranes causing them to lyse.
MX3: allows isolation of nucleic acids (DNA) from lipids, sugars, and proteins found in
cells.
What charge is DNA and therefore what part of the box is it attracted to (cathode or
anode)? Solved Answers It is negative so therefore it is attracted to the cathode.
What is the gel called in electrophoresis? Solved Answers Agarose gel.
What does the buffer do in gel electrophoresis? Solved Answers It allows the electric
current to flow through.
How to make 300 mL of 1X TAE buffer from 50X stock solution? Solved Answers
What were the purposes of the three buffers we used in Week 3 during our DNA prep?
Solved Answers Degrade RNA, lyse (break open) cells, cause large biomolecules to
crash out of solution
How did we isolate DNA from the buffer/lysate mixture in Week 3? Solved Answers
Using a column that temporarily bound DNA.
Solved Answers
What pipette and what setting would you use to get 1000 microliters (1 mL)? Solved
Answers p1000 and 100.
What pipette and what setting would you use to get 50 microliters (0.05 mL)? Solved
Answers p 200 and 050.
How to get the final percentage of each ingredient? Solved Answers Take the ingredient
total divided by the overall total.
What are the three kinds of agar plates used for in Week 5? Solved Answers Bacterial
growth.
What is the 50x TAE buffer used for in week 4? Solved Answers To make DNA gel.
What is a plasmid? Solved Answers A piece of DNA that can exist outside the cell's
chromosome.
What are the three buffers in Week 3, and what are their functions? Solved Answers
MX1: contains RNAse (enzyme degrading RNA)
MX2: contains detergent that disrupts bacterial cell membranes causing them to lyse.
MX3: allows isolation of nucleic acids (DNA) from lipids, sugars, and proteins found in
cells.
What charge is DNA and therefore what part of the box is it attracted to (cathode or
anode)? Solved Answers It is negative so therefore it is attracted to the cathode.
What is the gel called in electrophoresis? Solved Answers Agarose gel.
What does the buffer do in gel electrophoresis? Solved Answers It allows the electric
current to flow through.
How to make 300 mL of 1X TAE buffer from 50X stock solution? Solved Answers
What were the purposes of the three buffers we used in Week 3 during our DNA prep?
Solved Answers Degrade RNA, lyse (break open) cells, cause large biomolecules to
crash out of solution
How did we isolate DNA from the buffer/lysate mixture in Week 3? Solved Answers
Using a column that temporarily bound DNA.
