Florida Phlebotomy Technician Certification Exam Test
Questions And Correct Answers (Verified Answers) Plus
Rationales 2025/2026 Q&A | Instant Download Pdf
1. What is the primary purpose of a tourniquet during venipuncture?
To restrict venous blood flow to make veins more prominent
This helps dilate the veins, making them easier to locate and puncture for
blood collection.
2. Which of the following is the best site for routine venipuncture?
Median cubital vein
It is usually large, close to the surface, and less likely to be near nerves or
arteries, reducing patient discomfort and complications.
3. How long should a tourniquet be left on a patient during venipuncture?
No longer than 1 minute
Prolonged tourniquet application can cause hemoconcentration and affect
test results.
4. What is the correct order of draw for blood collection tubes?
Blood culture, coagulation (light blue), serum (red), heparin (green), EDTA
(lavender), glycolytic inhibitor (gray)
Following the order prevents cross-contamination of additives between
tubes.
,5. What does hemolysis mean in a blood sample?
Destruction of red blood cells
Hemolysis can falsely elevate or decrease certain test values,
compromising accuracy.
6. What is the recommended angle for needle insertion during venipuncture?
15 to 30 degrees
This angle allows smooth entry into the vein without going through the
other side.
7. What should you do if a patient feels faint during a blood draw?
Stop the procedure and have the patient lie down or sit with head
lowered
This helps prevent injury from fainting and allows for stabilization.
8. What is the best way to identify a patient before drawing blood?
Ask the patient to state their full name and date of birth, then verify with
the requisition form
Proper identification ensures the right patient’s blood is collected to avoid
errors.
9. What additive is in a lavender-top tube?
EDTA
EDTA prevents blood clotting by binding calcium, used for hematology
tests.
10.What is the correct method to cleanse a venipuncture site?
Cleanse with 70% isopropyl alcohol in a circular motion from inside out
This minimizes contamination and infection risk during the procedure.
, 11.How long should you allow the alcohol to dry before venipuncture?
At least 30 seconds
Incomplete drying can cause a burning sensation and hemolysis of the
sample.
12.Which of the following is an example of a PPE (Personal Protective
Equipment) used during phlebotomy?
Gloves
Gloves protect both the technician and patient from bloodborne
pathogens and contamination.
13.If a patient has an IV in the arm, where should you draw blood?
From the arm without the IV or below the IV site
Drawing above or from an arm with an IV can cause contamination or
diluted samples.
14.What is the appropriate action if a patient refuses blood collection?
Respect the patient’s decision and inform the healthcare provider
Patients have the right to refuse; documentation and communication are
essential.
15.What is the normal adult volume of blood drawn for a routine test?
5 to 10 milliliters
Excessive blood draws can cause anemia or discomfort; only the necessary
volume should be collected.
16.How should you dispose of a used needle?
In a sharps container without recapping
Recapping increases the risk of accidental needle sticks and infections.
Questions And Correct Answers (Verified Answers) Plus
Rationales 2025/2026 Q&A | Instant Download Pdf
1. What is the primary purpose of a tourniquet during venipuncture?
To restrict venous blood flow to make veins more prominent
This helps dilate the veins, making them easier to locate and puncture for
blood collection.
2. Which of the following is the best site for routine venipuncture?
Median cubital vein
It is usually large, close to the surface, and less likely to be near nerves or
arteries, reducing patient discomfort and complications.
3. How long should a tourniquet be left on a patient during venipuncture?
No longer than 1 minute
Prolonged tourniquet application can cause hemoconcentration and affect
test results.
4. What is the correct order of draw for blood collection tubes?
Blood culture, coagulation (light blue), serum (red), heparin (green), EDTA
(lavender), glycolytic inhibitor (gray)
Following the order prevents cross-contamination of additives between
tubes.
,5. What does hemolysis mean in a blood sample?
Destruction of red blood cells
Hemolysis can falsely elevate or decrease certain test values,
compromising accuracy.
6. What is the recommended angle for needle insertion during venipuncture?
15 to 30 degrees
This angle allows smooth entry into the vein without going through the
other side.
7. What should you do if a patient feels faint during a blood draw?
Stop the procedure and have the patient lie down or sit with head
lowered
This helps prevent injury from fainting and allows for stabilization.
8. What is the best way to identify a patient before drawing blood?
Ask the patient to state their full name and date of birth, then verify with
the requisition form
Proper identification ensures the right patient’s blood is collected to avoid
errors.
9. What additive is in a lavender-top tube?
EDTA
EDTA prevents blood clotting by binding calcium, used for hematology
tests.
10.What is the correct method to cleanse a venipuncture site?
Cleanse with 70% isopropyl alcohol in a circular motion from inside out
This minimizes contamination and infection risk during the procedure.
, 11.How long should you allow the alcohol to dry before venipuncture?
At least 30 seconds
Incomplete drying can cause a burning sensation and hemolysis of the
sample.
12.Which of the following is an example of a PPE (Personal Protective
Equipment) used during phlebotomy?
Gloves
Gloves protect both the technician and patient from bloodborne
pathogens and contamination.
13.If a patient has an IV in the arm, where should you draw blood?
From the arm without the IV or below the IV site
Drawing above or from an arm with an IV can cause contamination or
diluted samples.
14.What is the appropriate action if a patient refuses blood collection?
Respect the patient’s decision and inform the healthcare provider
Patients have the right to refuse; documentation and communication are
essential.
15.What is the normal adult volume of blood drawn for a routine test?
5 to 10 milliliters
Excessive blood draws can cause anemia or discomfort; only the necessary
volume should be collected.
16.How should you dispose of a used needle?
In a sharps container without recapping
Recapping increases the risk of accidental needle sticks and infections.
