8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
MB (ASCP) Exam EXAM Questions & Answers |
100% Verified solutions | 2025\2026 Latest!!
Save
Terms in this set (332)
Predominant form of DNA Right-handed B
DNA and histone Ionic
interaction is due to what
type of bond?
What stains to the minor SYBR Green
groove of DNA duplex?
What is the most abundant Ribosomal RNA (rRNA)
type of RNA?
What does RNA act as in a Genome and mRNA
retrovirus?
What increases half-life of 5' Methyl Cap
mRNA?
What kind of DNA is most Euchromatin
likely to be
transcriptionally active?
What is the most common Satellite DNA
type of eukaryotic DNA?
What specimen has the White Blood Cells
most DNA according to
molecular weight?
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,8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
What amino acids Lysine and Arginine
compose histones?
Amino acid that all Methionine
proteins start with:
What protects the DNA Single-stranded binding protein
strand during replication?
Leading Strand 3' to 5' template
Lagging Strand 5' to 3' template
What DNA strand is being Antisense Strand
copied in transcription?
First step after newly Addition of poly(A) tail
formed mRNA is made?
Every time the Telomerase
chromosome is copied it
would become shorter if
not for what enzyme?
What polymerase has Beta/Epsilon
repair activity?
What polymerase acts on Delta
leading strand?
What polymerase acts on Alpha
lagging strand?
Which polymerase is used Gamma
for mitochondrial
replication and repair?
What polymerase binds Topoisomerase
the 5' end to 3' end of
dsDNA?
Which RNA polymerase is RNA Pol II
mainly for transcription?
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,8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
Which polymerase is RNA Pol II
involved with mRNA
synthesis?
Diethyl pyrocarbonate (DEPC) or bake glassware for
What inactivates RNAses?
4 to 6 hours at 400C
What type of restriction Restriction Enzyme Type II
enzyme recognizes
palindromic sites?
What type of restriction Restriction Enzyme Type II
enzyme lacks methylation
activity?
What is necessary to make Reverse transcriptase
cDNA?
Number of SNPs in a 11 billion (10^7)
human genome?
How many genes (base 3 billion base pairs
pairs) are in a human
haploid genome?
The polymerization of Catalyst (APS or TEMED) or Light activation
acrylamide requires:
How does agarose gel Inversely proportional
pore size relate to
concentration?
What is denatured during Hydrogen bonds (inter-strand bonds)
a southern blot?
Melting temperature Tm = (4C x GC Pairs) + (2C x AT pairs)
formula
Which is likely to interfere 5' G
with fluorescent probe?
Proper GC content of 35-65%
primer
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, 8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Inhibitors of PCR
Bromophenol Blue
Order to add to a PCR Master mix > patient sample > positive control >
reaction: negative control
Pre Area Positive Pressure
Post Area Negative Pressure
Which PCR requires two Nested PCR
steps for specificity?
Which microorganism is DNA or RNA
detected by branched
DNA amplification?
Which technique uses Hybrid Capture Assay
RNA, antibodies, and
DNA?
Use of bisulfite treatment Bisulfite DNA
of DNA to determine its
pattern of methylation
What tests are isothermal? NASBA, TMA, & SDA
Which leukemia is AML
associated with FLT3
gene?
T-cell gene Sezary Syndrome
rearrangement is seen in
what malignancy?
Which translocation t 15;17
causes Acute
Premyelocytic Leukemia?
Ewing Sarcoma t 11;22
Synovial Sarcoma t X;18
Follicular Lymphoma t 14;18
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MB (ASCP) Exam EXAM Questions & Answers |
100% Verified solutions | 2025\2026 Latest!!
Save
Terms in this set (332)
Predominant form of DNA Right-handed B
DNA and histone Ionic
interaction is due to what
type of bond?
What stains to the minor SYBR Green
groove of DNA duplex?
What is the most abundant Ribosomal RNA (rRNA)
type of RNA?
What does RNA act as in a Genome and mRNA
retrovirus?
What increases half-life of 5' Methyl Cap
mRNA?
What kind of DNA is most Euchromatin
likely to be
transcriptionally active?
What is the most common Satellite DNA
type of eukaryotic DNA?
What specimen has the White Blood Cells
most DNA according to
molecular weight?
https://quizlet.com/1065200562/mb-ascp-exam-exam-questions-answers-100-verified-solutions-20252026-latest-flash-cards/?new 1/32
,8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
What amino acids Lysine and Arginine
compose histones?
Amino acid that all Methionine
proteins start with:
What protects the DNA Single-stranded binding protein
strand during replication?
Leading Strand 3' to 5' template
Lagging Strand 5' to 3' template
What DNA strand is being Antisense Strand
copied in transcription?
First step after newly Addition of poly(A) tail
formed mRNA is made?
Every time the Telomerase
chromosome is copied it
would become shorter if
not for what enzyme?
What polymerase has Beta/Epsilon
repair activity?
What polymerase acts on Delta
leading strand?
What polymerase acts on Alpha
lagging strand?
Which polymerase is used Gamma
for mitochondrial
replication and repair?
What polymerase binds Topoisomerase
the 5' end to 3' end of
dsDNA?
Which RNA polymerase is RNA Pol II
mainly for transcription?
https://quizlet.com/1065200562/mb-ascp-exam-exam-questions-answers-100-verified-solutions-20252026-latest-flash-cards/?new 2/32
,8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
Which polymerase is RNA Pol II
involved with mRNA
synthesis?
Diethyl pyrocarbonate (DEPC) or bake glassware for
What inactivates RNAses?
4 to 6 hours at 400C
What type of restriction Restriction Enzyme Type II
enzyme recognizes
palindromic sites?
What type of restriction Restriction Enzyme Type II
enzyme lacks methylation
activity?
What is necessary to make Reverse transcriptase
cDNA?
Number of SNPs in a 11 billion (10^7)
human genome?
How many genes (base 3 billion base pairs
pairs) are in a human
haploid genome?
The polymerization of Catalyst (APS or TEMED) or Light activation
acrylamide requires:
How does agarose gel Inversely proportional
pore size relate to
concentration?
What is denatured during Hydrogen bonds (inter-strand bonds)
a southern blot?
Melting temperature Tm = (4C x GC Pairs) + (2C x AT pairs)
formula
Which is likely to interfere 5' G
with fluorescent probe?
Proper GC content of 35-65%
primer
https://quizlet.com/1065200562/mb-ascp-exam-exam-questions-answers-100-verified-solutions-20252026-latest-flash-cards/?new 3/32
, 8/12/25, 6:53 AM MB (ASCP) Exam EXAM Questions & Answers | 100% Verified solutions | 2025\2026 Latest!! Flashcards | Quizlet
Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Inhibitors of PCR
Bromophenol Blue
Order to add to a PCR Master mix > patient sample > positive control >
reaction: negative control
Pre Area Positive Pressure
Post Area Negative Pressure
Which PCR requires two Nested PCR
steps for specificity?
Which microorganism is DNA or RNA
detected by branched
DNA amplification?
Which technique uses Hybrid Capture Assay
RNA, antibodies, and
DNA?
Use of bisulfite treatment Bisulfite DNA
of DNA to determine its
pattern of methylation
What tests are isothermal? NASBA, TMA, & SDA
Which leukemia is AML
associated with FLT3
gene?
T-cell gene Sezary Syndrome
rearrangement is seen in
what malignancy?
Which translocation t 15;17
causes Acute
Premyelocytic Leukemia?
Ewing Sarcoma t 11;22
Synovial Sarcoma t X;18
Follicular Lymphoma t 14;18
https://quizlet.com/1065200562/mb-ascp-exam-exam-questions-answers-100-verified-solutions-20252026-latest-flash-cards/?new 4/32
