Portage Microbiology BIOD171 Exam 4 actual
Study online at https://quizlet.com/_hlz9o4
True
Growth media does not contain restric-
tive factors, while selective media is best
True or False: Differential media is best used to encourage the growth of one mi-
suited for distinguishing between two crobe while simultaneously discouraging
similar species of bacteria the growth of the other. Since two similar
species of microbes are being studied
they must be differentiated under similar
but just slightly different conditions (dif-
ferential media).
A researcher is asked to determine
which of two vials contains E coli
and which contains salmonella. Knowing
both are Gram-negative while only one Differential media
of them is capable of fermenting lactose,
which type of media would be best suit-
ed?
A fastidious microbe is an organism with
complex growth requirements such that
What are the requirements of a fastidi- if absent it will not grow. Enriched medias
ous microbe? thus contain these specific and essential
nutrients required for the growth of a par-
ticular subset of microorganisms.
True or False: LB agar is classified as a
False.
selective, non-differential media.
The polysaccharide-based hardening
agent added to LB media to produce LB Seaweed (algae) extract.
agar is derived from what?
Blood agar is which type of medium?
Select all that apply.
Enriched
Selective
Differential
Enriched
Selective and Differential
Differential
1/5
, Portage Microbiology BIOD171 Exam 4 actual
Study online at https://quizlet.com/_hlz9o4
Match the following hemolytic class with
1. Alpha hemolysis --> B. Green-
its description of activity.
ish-brown color
1. Alpha hemolysis A. No change.
2. Beta hemolysis ---> C. Distinct zone of
2. Beta hemolysis B. Greenish-brown
clearing
color
3. Gamma hemolysis C. Distinct zone of
3. Gamma hemolysis --> A. No change.
clearing
Blood agar
Columbia CNA agar is most closely re-
CNA agar is similar to BAP as it is also
lated to which media:
enriched with blood and allows for differ-
entiation based on hemolytic patterns.
False
True or False: Chocolate agar gets its Chocolate (cocoa) is never added to
brown color from cocoa to produce an the media. The name is derived simply
enriched media. based on the color that actually comes
from the presence of 'cooked' (lysed) red
blood cells in the media.
No. A Gram stain would not be neces-
sary, as only Gram-Negative microbes
An unknown microbe is streaked onto
will grow on MacConkey agar.
a MacConkey agar plate. After an
overnight incubation at 37°C, growth is
No, because MacConkey agar only
observed. Would a Gram stain be neces-
grows gram-negative microbes so there-
sary? Why or why not?
fore if there is a growth it is gram-nega-
tive. (my answer, full points)
The microbe should be plated on
SMAC (Sorbitol-MacConkey agar) as
it is specifically formulated to detect
In an attempt to detect the presence of O157:H7. Pathogenic E. coli (O157:H7)
the pathogenic strain of E. coli O157:H7, cannot ferment sorbitol while non-path-
a researcher spread a culture onto ogenic E. coli can ferment both soribitol
a MacConkey agar with failed results. and lactose. Therefore, colonies that fer-
ment (acidic conditions; non-pathogen-
ic) can be differentiated from non-fer-
menters (neutral to basic conditions;
2/5
Study online at https://quizlet.com/_hlz9o4
True
Growth media does not contain restric-
tive factors, while selective media is best
True or False: Differential media is best used to encourage the growth of one mi-
suited for distinguishing between two crobe while simultaneously discouraging
similar species of bacteria the growth of the other. Since two similar
species of microbes are being studied
they must be differentiated under similar
but just slightly different conditions (dif-
ferential media).
A researcher is asked to determine
which of two vials contains E coli
and which contains salmonella. Knowing
both are Gram-negative while only one Differential media
of them is capable of fermenting lactose,
which type of media would be best suit-
ed?
A fastidious microbe is an organism with
complex growth requirements such that
What are the requirements of a fastidi- if absent it will not grow. Enriched medias
ous microbe? thus contain these specific and essential
nutrients required for the growth of a par-
ticular subset of microorganisms.
True or False: LB agar is classified as a
False.
selective, non-differential media.
The polysaccharide-based hardening
agent added to LB media to produce LB Seaweed (algae) extract.
agar is derived from what?
Blood agar is which type of medium?
Select all that apply.
Enriched
Selective
Differential
Enriched
Selective and Differential
Differential
1/5
, Portage Microbiology BIOD171 Exam 4 actual
Study online at https://quizlet.com/_hlz9o4
Match the following hemolytic class with
1. Alpha hemolysis --> B. Green-
its description of activity.
ish-brown color
1. Alpha hemolysis A. No change.
2. Beta hemolysis ---> C. Distinct zone of
2. Beta hemolysis B. Greenish-brown
clearing
color
3. Gamma hemolysis C. Distinct zone of
3. Gamma hemolysis --> A. No change.
clearing
Blood agar
Columbia CNA agar is most closely re-
CNA agar is similar to BAP as it is also
lated to which media:
enriched with blood and allows for differ-
entiation based on hemolytic patterns.
False
True or False: Chocolate agar gets its Chocolate (cocoa) is never added to
brown color from cocoa to produce an the media. The name is derived simply
enriched media. based on the color that actually comes
from the presence of 'cooked' (lysed) red
blood cells in the media.
No. A Gram stain would not be neces-
sary, as only Gram-Negative microbes
An unknown microbe is streaked onto
will grow on MacConkey agar.
a MacConkey agar plate. After an
overnight incubation at 37°C, growth is
No, because MacConkey agar only
observed. Would a Gram stain be neces-
grows gram-negative microbes so there-
sary? Why or why not?
fore if there is a growth it is gram-nega-
tive. (my answer, full points)
The microbe should be plated on
SMAC (Sorbitol-MacConkey agar) as
it is specifically formulated to detect
In an attempt to detect the presence of O157:H7. Pathogenic E. coli (O157:H7)
the pathogenic strain of E. coli O157:H7, cannot ferment sorbitol while non-path-
a researcher spread a culture onto ogenic E. coli can ferment both soribitol
a MacConkey agar with failed results. and lactose. Therefore, colonies that fer-
ment (acidic conditions; non-pathogen-
ic) can be differentiated from non-fer-
menters (neutral to basic conditions;
2/5
