MB ASCP Demo Practice Exam Questions with 100%
Correct Answers
1. Given the anti-sense strand of DNA: 5'- C. The probe
AATTGCCGACATAGAT-3' which is the D. All of the above - ANSWER -A. The
appropriate sense strand? target
A. 5'-ATCTATGTCGGCAATT-3'
B. 5'-TTAACGGCTGTATCTA-3'
C. 5'-AATTGCCGACATAGAT-3'
D. 5'-GAGCACGCTATCTTAT-3' - 6. All of the following are components of nucleic
ANSWER -A. 5'-ATCTATGTCGGCAATT-3' acids, EXCEPT:
A. Phosphate group
B. Sugar (ribose or deoxyribose)
C. Nitrogenous base (A, G, C, T)
2. Consider the probe sequence, D. Formamide - ANSWER -D. Formamide
CTACCGTAATATTCGACCGT, to be used in a
hybridization procedure. What is the melting
temperature, Tm, of the sequence?
A. 60°C 7. Which of the following is least likely to increase
B. 58°C the specificity of a PCR reaction?
C. 64°C A. Decrease the annealing temperature of
D. 62°C - ANSWER -B. 58°C primers
B. Incorporate DMSO or formamide into the PCR
reaction
C. Use a high fidelity polymerase
3. What assay amplifies the target using a D. Use nested PCR - ANSWER -A.
combination of a three-enzyme system? Decrease the annealing temperature of primers
A. Branched DNA
B. TMA
C. PCR
D. NASBA 8. There are many types of RNA molecules, each
E. LCR - ANSWER -D. NASBA of which has a specific function within the cell.
Which type of RNA molecule is responsible for
transporting amino acids during protein
synthesis?
4. All of the following enzymes are part of the A. Transfer RNA (tRNA)
DNA replication machinery, EXCEPT: B. Ribosomal RNA (rRNA)
A. Ligase C. Messenger RNA (mRNA)
B. DNA polymerase D. Small Nuclear RNA (snRNA)
C. Luciferase E. MicroRNA (miRNA) - ANSWER -A.
D. Helicase - ANSWER -C. Luciferase Transfer RNA (tRNA)
5. In real-time PCR, what is amplified? 9. Which of the following is not a source of DNA
A. The target damage?
B. The signal A. Chemical damage
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Correct Answers
1. Given the anti-sense strand of DNA: 5'- C. The probe
AATTGCCGACATAGAT-3' which is the D. All of the above - ANSWER -A. The
appropriate sense strand? target
A. 5'-ATCTATGTCGGCAATT-3'
B. 5'-TTAACGGCTGTATCTA-3'
C. 5'-AATTGCCGACATAGAT-3'
D. 5'-GAGCACGCTATCTTAT-3' - 6. All of the following are components of nucleic
ANSWER -A. 5'-ATCTATGTCGGCAATT-3' acids, EXCEPT:
A. Phosphate group
B. Sugar (ribose or deoxyribose)
C. Nitrogenous base (A, G, C, T)
2. Consider the probe sequence, D. Formamide - ANSWER -D. Formamide
CTACCGTAATATTCGACCGT, to be used in a
hybridization procedure. What is the melting
temperature, Tm, of the sequence?
A. 60°C 7. Which of the following is least likely to increase
B. 58°C the specificity of a PCR reaction?
C. 64°C A. Decrease the annealing temperature of
D. 62°C - ANSWER -B. 58°C primers
B. Incorporate DMSO or formamide into the PCR
reaction
C. Use a high fidelity polymerase
3. What assay amplifies the target using a D. Use nested PCR - ANSWER -A.
combination of a three-enzyme system? Decrease the annealing temperature of primers
A. Branched DNA
B. TMA
C. PCR
D. NASBA 8. There are many types of RNA molecules, each
E. LCR - ANSWER -D. NASBA of which has a specific function within the cell.
Which type of RNA molecule is responsible for
transporting amino acids during protein
synthesis?
4. All of the following enzymes are part of the A. Transfer RNA (tRNA)
DNA replication machinery, EXCEPT: B. Ribosomal RNA (rRNA)
A. Ligase C. Messenger RNA (mRNA)
B. DNA polymerase D. Small Nuclear RNA (snRNA)
C. Luciferase E. MicroRNA (miRNA) - ANSWER -A.
D. Helicase - ANSWER -C. Luciferase Transfer RNA (tRNA)
5. In real-time PCR, what is amplified? 9. Which of the following is not a source of DNA
A. The target damage?
B. The signal A. Chemical damage
1/2
