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ASCP Practice Questions – Histotechnology with 100% Verified Answers Graded A

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This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. - ANSWER -Acetic acid Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. - ANSWER -Glutaraldehyde This fixative is the smallest dialdehyde and is used in the textile industry for imparting wrinkle resistance and permanent press creases. It has replaced formaldehyde in some histopathology laboratories because it is much less toxic. - ANSWE

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ASCP Practice Questions – Histotechnology with 100%
Verified Answers Graded A
This fixative does not fix or destroy
carbohydrates, and it does not fix lipids. It
penetrates very rapidly and leaves tissue very This chelating agent binds calcium ions and is
soft. It is added to many fixative mixtures one method used for decalcifying tissues. -
because of its ability to fix nuclei. - ANSWER -Ethylenediaminetetraacetic acid
ANSWER -Acetic acid (EDTA)


Techniques using Schiff reagent, such as the This microscope is used for the examination of
periodic acid-Schiff (PAS) stain, cannot be used unstained specimens, especially unstained living
on __________ fixed tissues because false cells, and allows almost transparent objects to be
positive results will be obtained. - seen clearly. - ANSWER -Phase-contrast
ANSWER -Glutaraldehyde microscope


This fixative is the smallest dialdehyde and is This piece of equipment is a refrigerated
used in the textile industry for imparting wrinkle chamber containing a microtome
resistance and permanent press creases. It has that is used to cut frozen sections. -
replaced formaldehyde in some histopathology ANSWER -Cryostat
laboratories because it is much less toxic. -
ANSWER -Glyoxal
OSHA defines ____________ as chemicals that
may cause acute or chronic health effects in
Today, _________ has/have found use not only exposed employees. - ANSWER -
in combination with formaldehyde for routine Hazardous chemicals
fixation, but as a substitute for mercury in the B-5
solution commonly used for the fixation of lymph
node and bone marrow tissues - The lowest dose of a substance that will produce
ANSWER -Zinc salts any toxic effect in humans when introduced by
any route other than inhalation is the: -
ANSWER -Toxic dose low
If unfixed tissue is to be held for several days or
transported over a long distance then this
transport medium is recommended. - The right yellow diamond in the NFPA coded
ANSWER -Michel transport medium labeling system carries a chemical's
____________ information. - ANSWER -
Reactivity
Tissue processing is usually considered to
include: - ANSWER -Dehydration, clearing,
infiltration This dense, rounded, and usually intensely
basophilic mass in the nucleus of
a cell consists of 80% to 90% protein and
The most widely used clearing agent in histology produces most if not all of the ribosomal RNA. -
today is: - ANSWER -Xylene ANSWER -Nucleolus
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