ASCP CG Certification Practice Questions with 100% Verified
Answers Graded A+
What is the best agent to use against ANSWER -Dissection
mycoplasma? - ANSWER -BM cyclin - a
combination of two antibiotics (tiamulin and
minocycline) that effecticely targets mycoplasma Which cell type is most problematic if found in a
growth CVS (chorionic villus) sample? - ANSWER -
Decidua (maternal tissue)
For successful harvest, each new batch of
hypotonic solution should be evaluated by: - When looking at a slide under a phase contrast
ANSWER -comparing the new lot to a lot scope and a lot of cytoplasm is still seen around
already in use the mets, what is the most likely cause? -
ANSWER -innappropriate slide drying time
Colagenase, pronase, and trypsin are: -
ANSWER -Proteolytic enzymes - break What will help prevent microbial contamination
down tissues into single-cell suspensions and cross contamination of cultures? -
ANSWER -Asceptic technique
When harvesting peripheral blood cultures,
asceptic technique no longer matters following When there are not enough mets to complete the
incubation with: - ANSWER -Colcemid - study of a patient/case, what needs to be done
mitotic spindle poison that halts cells at next? - ANSWER -prepare additional slides
metaphase from any remaining sample
Samples that arrive at the cytogenetics lab When looking at a dropped slide under a phase
should first be: - ANSWER -Logged in to scope and mets are very spread out and sparse,
the system what could help fix the issue? - ANSWER -
Increading the drying time by using warm slides
Phytohemagglutinin (PHA) stimulates which type
of cells into division? - ANSWER -T-cell What is true of an inverted microscope? -
lymphocytes ANSWER -the objectives are beneath the
stage
To avoid culture failure, monitoring reagents is
crucial. When logging a culture failure, what is High-resolution banding is indicated for a
the MOST important to document? - possible diagnosis of: - ANSWER -
ANSWER -Expiration dates of reagents DiGenorge syndrome -- microdeletion of 22q11,
used also called velocardiofacial syndrome
"velo" - soft palette,
Which type of microscope is used when cleaning "cardio" - congenital heart defects,
a CVS (chorionic villus) sample? - "facial" - unique abnormal facial features, short
, ASCP CG Certification Practice Questions with 100% Verified
Answers Graded A+
philtrum (upper lip indent), tubular nose, wide success rate and abnormality detection in
spread eyes, etc spontaneous abortion miscarriages? -
ANSWER -Chorionic villi
When verifying if a specimen is appropriate for
cytogenetic analysis, what is the MOST What is a possible cause for a drying oven to not
important data to accompany the specimen? - maintain a proper temperature? -
ANSWER -Reason for referral ANSWER -The door does not seal properly
Which type of specimen should be requested for If a tech with a cold and sneezing does not take
a cytogenetic analysis for turner syndrome? - proper precautions while setting up amniotic fluid
ANSWER -Heparinized peripheral blood samples, the specimen could become
stored at room temperature contaminated with: - ANSWER -
Mycoplasma
(NOT FROZEN)
--> the most common contaminant by a sick tech
and can be detected through a specific stain
A blood sample can be used to study the used on the sample
cytogenetics of a malignancy only if: -
ANSWER -Dividing leukemic cells are mycoplasma can induce abnormalities in a
present in circulation sample, so it is important to avoid contamination
All of the cultures in a CO2 incubator failed, what What about the metaphases on a slide are
aspects of the incubator are important to monitor affected by slide making? - ANSWER -Cell
to assess the source of the problem? - density - thru dilution of the sample with fix
ANSWER -Temperature,
CO2 level, and Banding - based on how dark/pale/refractive they
Humidity level are affects trypsin time
Chromosome spreading - affected by drying time
The most important factor affecting the quality of
chromosomes viewed under a phase contrast
scope is: - ANSWER -Slide drying after the What test(s) should be ordered on a newborn
cells have been dropped with suspected Prader-Willi syndrome? -
ANSWER -High resolution banding and
FISH for the Prader-Willi locus on 15q11.2
What is the modal number (default number of
chromosomes in a cell) for a Down syndrome
translocation carrier? - ANSWER -45 When capturing a metaphase, a tech cannot get
the met into focus, what is the most likely cause?
- ANSWER -The camera is not parfocal (in
What is the best tissue to use for both a good focus with the microscpe objectives) with the
Answers Graded A+
What is the best agent to use against ANSWER -Dissection
mycoplasma? - ANSWER -BM cyclin - a
combination of two antibiotics (tiamulin and
minocycline) that effecticely targets mycoplasma Which cell type is most problematic if found in a
growth CVS (chorionic villus) sample? - ANSWER -
Decidua (maternal tissue)
For successful harvest, each new batch of
hypotonic solution should be evaluated by: - When looking at a slide under a phase contrast
ANSWER -comparing the new lot to a lot scope and a lot of cytoplasm is still seen around
already in use the mets, what is the most likely cause? -
ANSWER -innappropriate slide drying time
Colagenase, pronase, and trypsin are: -
ANSWER -Proteolytic enzymes - break What will help prevent microbial contamination
down tissues into single-cell suspensions and cross contamination of cultures? -
ANSWER -Asceptic technique
When harvesting peripheral blood cultures,
asceptic technique no longer matters following When there are not enough mets to complete the
incubation with: - ANSWER -Colcemid - study of a patient/case, what needs to be done
mitotic spindle poison that halts cells at next? - ANSWER -prepare additional slides
metaphase from any remaining sample
Samples that arrive at the cytogenetics lab When looking at a dropped slide under a phase
should first be: - ANSWER -Logged in to scope and mets are very spread out and sparse,
the system what could help fix the issue? - ANSWER -
Increading the drying time by using warm slides
Phytohemagglutinin (PHA) stimulates which type
of cells into division? - ANSWER -T-cell What is true of an inverted microscope? -
lymphocytes ANSWER -the objectives are beneath the
stage
To avoid culture failure, monitoring reagents is
crucial. When logging a culture failure, what is High-resolution banding is indicated for a
the MOST important to document? - possible diagnosis of: - ANSWER -
ANSWER -Expiration dates of reagents DiGenorge syndrome -- microdeletion of 22q11,
used also called velocardiofacial syndrome
"velo" - soft palette,
Which type of microscope is used when cleaning "cardio" - congenital heart defects,
a CVS (chorionic villus) sample? - "facial" - unique abnormal facial features, short
, ASCP CG Certification Practice Questions with 100% Verified
Answers Graded A+
philtrum (upper lip indent), tubular nose, wide success rate and abnormality detection in
spread eyes, etc spontaneous abortion miscarriages? -
ANSWER -Chorionic villi
When verifying if a specimen is appropriate for
cytogenetic analysis, what is the MOST What is a possible cause for a drying oven to not
important data to accompany the specimen? - maintain a proper temperature? -
ANSWER -Reason for referral ANSWER -The door does not seal properly
Which type of specimen should be requested for If a tech with a cold and sneezing does not take
a cytogenetic analysis for turner syndrome? - proper precautions while setting up amniotic fluid
ANSWER -Heparinized peripheral blood samples, the specimen could become
stored at room temperature contaminated with: - ANSWER -
Mycoplasma
(NOT FROZEN)
--> the most common contaminant by a sick tech
and can be detected through a specific stain
A blood sample can be used to study the used on the sample
cytogenetics of a malignancy only if: -
ANSWER -Dividing leukemic cells are mycoplasma can induce abnormalities in a
present in circulation sample, so it is important to avoid contamination
All of the cultures in a CO2 incubator failed, what What about the metaphases on a slide are
aspects of the incubator are important to monitor affected by slide making? - ANSWER -Cell
to assess the source of the problem? - density - thru dilution of the sample with fix
ANSWER -Temperature,
CO2 level, and Banding - based on how dark/pale/refractive they
Humidity level are affects trypsin time
Chromosome spreading - affected by drying time
The most important factor affecting the quality of
chromosomes viewed under a phase contrast
scope is: - ANSWER -Slide drying after the What test(s) should be ordered on a newborn
cells have been dropped with suspected Prader-Willi syndrome? -
ANSWER -High resolution banding and
FISH for the Prader-Willi locus on 15q11.2
What is the modal number (default number of
chromosomes in a cell) for a Down syndrome
translocation carrier? - ANSWER -45 When capturing a metaphase, a tech cannot get
the met into focus, what is the most likely cause?
- ANSWER -The camera is not parfocal (in
What is the best tissue to use for both a good focus with the microscpe objectives) with the
