HISTOLOGY ASCP 2026 EXAM TEST WITH
QUESTIONS AND CORRECT ANSWERS
GRADED A+
Carnoy solution is a combination of which chemicals?
A. Absolute alcohol, acetone, and glacial acetic acid
B. Cedar wood oil, absolute alcohol, and glacial acetic acid
C. Acetone, chloroform, and absolute alcohol
D. Chloroform, glacial acetic acid, and absolute alcohol Correct
Answer Chloroform, glacial acetic acid, and absolute alcohol
Tissue stored for long periods of time in unbuffered formalin or in
acetate formalin may show brown, crystalline pigment in stained
sections. To remove this pigment prior to staining it is necessary
to treat the microscopic section with:
A. Saturated alcoholic picric acid
B. Alcoholic lithium chloride
C. Iodine and sodium thiosulfate
D. Potassium permanganate and oxalic acid Correct Answer
Saturated alcoholic picric acid
,For good fixation of tissue with osmium tetroxide for electron
microscopy, it is recommended that the tissue segment be no
larger than:
A. 1 mm cubed
B. 2 mm cubed
C. 2 cm cubed
D. 2 cm cubed Correct Answer 1 mm cubed
Following fixation with Bouin solution, tissue should be washed
with:
A. Absolute alcohol
B. 50% to 70% alcohol
C. 20% to 40% alcohol
D. Saline solution Correct Answer 50% to 70% alcohol
The PTAH staining technique would require postfixation, or
mordanting, if the tissue were originally fixed in:
A. Bouin
B. Zenker
C. Gendre
D. Formalin Correct Answer Formalin
Absolute ethanol is a poor choice for the fixation of:
,A. Glycogen
B. Pigments
C. Lipids
D. Blood smears Correct Answer lipids
Which of the following fixatives may give false positive results in
some carbohydrate techniques?
A. NBF
B. Bouin solution
C. Gendre solution
D. Glutaraldehyde Correct Answer Glutaraldehyde
It is necessary to adjust the pH of most formalin solutions
because of the presence of:
A. Methanol
B. Formic acid
C. Paraformaldehyde
D. Carbon dioxide Correct Answer formic acid
The rate of fixation varies with the fixative and also with the:
A. Time placed in the fixative of choice
B. Expected completion time of the report
C. Anticipated special stains needed
, D. Temperature of the fixative solution Correct Answer
Temperature of the fixative solution
Calcium-formalin fixative is recommended for the BEST
preservation and subsequent demonstration of:
A. Glycogen
B. Phospholipids
C. Amyloid
D. Estrogen receptors Correct Answer Phospholipids
Ultrastructural preservation will be very poor following fixation in:
A. Zamboni PAF
B. 2% buffered glutaraldehyde
C. Osmium tetroxide
D. 10% aqueous formalin Correct Answer 10% aqueous formalin
For most fixatives, the volume of fixing fluid in relation to the
volume of tissue should be:
A. 2 to 5 times
B. 6 to 9 times
C. 10 to 14 times
D. 15 to 20 times Correct Answer 15 to 20 times
QUESTIONS AND CORRECT ANSWERS
GRADED A+
Carnoy solution is a combination of which chemicals?
A. Absolute alcohol, acetone, and glacial acetic acid
B. Cedar wood oil, absolute alcohol, and glacial acetic acid
C. Acetone, chloroform, and absolute alcohol
D. Chloroform, glacial acetic acid, and absolute alcohol Correct
Answer Chloroform, glacial acetic acid, and absolute alcohol
Tissue stored for long periods of time in unbuffered formalin or in
acetate formalin may show brown, crystalline pigment in stained
sections. To remove this pigment prior to staining it is necessary
to treat the microscopic section with:
A. Saturated alcoholic picric acid
B. Alcoholic lithium chloride
C. Iodine and sodium thiosulfate
D. Potassium permanganate and oxalic acid Correct Answer
Saturated alcoholic picric acid
,For good fixation of tissue with osmium tetroxide for electron
microscopy, it is recommended that the tissue segment be no
larger than:
A. 1 mm cubed
B. 2 mm cubed
C. 2 cm cubed
D. 2 cm cubed Correct Answer 1 mm cubed
Following fixation with Bouin solution, tissue should be washed
with:
A. Absolute alcohol
B. 50% to 70% alcohol
C. 20% to 40% alcohol
D. Saline solution Correct Answer 50% to 70% alcohol
The PTAH staining technique would require postfixation, or
mordanting, if the tissue were originally fixed in:
A. Bouin
B. Zenker
C. Gendre
D. Formalin Correct Answer Formalin
Absolute ethanol is a poor choice for the fixation of:
,A. Glycogen
B. Pigments
C. Lipids
D. Blood smears Correct Answer lipids
Which of the following fixatives may give false positive results in
some carbohydrate techniques?
A. NBF
B. Bouin solution
C. Gendre solution
D. Glutaraldehyde Correct Answer Glutaraldehyde
It is necessary to adjust the pH of most formalin solutions
because of the presence of:
A. Methanol
B. Formic acid
C. Paraformaldehyde
D. Carbon dioxide Correct Answer formic acid
The rate of fixation varies with the fixative and also with the:
A. Time placed in the fixative of choice
B. Expected completion time of the report
C. Anticipated special stains needed
, D. Temperature of the fixative solution Correct Answer
Temperature of the fixative solution
Calcium-formalin fixative is recommended for the BEST
preservation and subsequent demonstration of:
A. Glycogen
B. Phospholipids
C. Amyloid
D. Estrogen receptors Correct Answer Phospholipids
Ultrastructural preservation will be very poor following fixation in:
A. Zamboni PAF
B. 2% buffered glutaraldehyde
C. Osmium tetroxide
D. 10% aqueous formalin Correct Answer 10% aqueous formalin
For most fixatives, the volume of fixing fluid in relation to the
volume of tissue should be:
A. 2 to 5 times
B. 6 to 9 times
C. 10 to 14 times
D. 15 to 20 times Correct Answer 15 to 20 times
