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Freshman Biology Final Exam 2025–2026 | High School & College Bio 101 Test Bank, Questions, Study Guide & Answer Key PDF

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Prepare for your Freshman Biology Final Exam with this 2025–2026 study pack. Includes, short answers, and a full answer key. Perfect for high school biology, introductory college biology (Bio 101), and standardized test prep. Download PDF version for instant review.

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Institution
Freshman Bio
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Freshman Bio

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Light-independent reactions
What step in photosynthesis may occur
during day and night?

deep ocean hydrothermal vents
Where is the bacteria involved in
chemosynthesis often found?

True
True/False:
NADPH stores the energy from excited
electrons in chemical bonds.

Heterotrophs
__ are organisms which must consume
organic molecules for energy.

The dark reaction receives energy from the ATP
light reaction in the form of

chemiosmotic gradient
Which process creates energy used by
ATP synthase to chemically bond a
phosphate group to ADP, synthesizing ATP
in the light reactions of photosynthesis?




RuBisCo
Which enzyme catalyzes the reaction that
combines CO2with a 5-carbon sugar,
ribulose bisphosphate, to initiate the Calvin
Cycle?

a) NADPH and ATP are used

Which of the following is NOT one of the
major events that occur during the light
reaction of photosynthesis?
a) NADPH and ATP are used
b) Oxygen gas is released
c) water is split
d) NADPH and ATP are produced
e) All of the above occur during the light
reaction of photosynthesis



Photolysis (splitting) of water
What is the purpose of sunlight in the
process of photosynthesis?

True
True/False:
Autotrophs transform low-energy inorganic
CO2into high-energy organic molecules
like glucose.


Photosynthesis occurs in the leaf __ Palisade layer

True/False: The Calvin cycle occurs in the False
thylakoid membrane

Which pigment molecule in plant cells chlorophyll
absorbs light?

Which part of a molecule does sunlight electron
"excite"?

What did Melvin Calvin discover? The light-independent reactions of photosynthesis

, c) Both a and b
Which of the following is performed by
leaves?
a) Photosynthesis
b) Gas exchange
c) Both a and b
d) None of the above


Glucose
What is the product of photosynthesis that
is the food for the autotroph that produces
it?

True
True/False:
Plants have chloroplasts and mitochondria
in their cells.


How does carbon dioxide (CO2) enter a Through pores known as stomata
leaf?

True
True/False:
Chloroplasts are green because they
contain chlorophyll pigment.

d) all of the above
Besides sunlight, which of the following
is/are sources of energy used by some
living organisms?
a) Hydrogen sulfide
b) methane
c) hydrogen gas
d) all of the above


6 CO2+ 6 H2O + light energy —> C6H12O6+ 6O2
What is the chemical equation of
photosynthesis?

When is ATP produced in photosynthesis? Light reactions

The Calvin cycle is part of photosynthesis, while the Krebs cycle is part of cellular
How would you best differentiate between respiration
the Krebs cycle and the Calvin cycle?


An environmental hazard that causes Carcinogen
cancer is known as a/n

Nucleus
Where does transcription take place in
eukaryotic cells?

no bacteria

In the pGLO transformation experiment,
E.coli bacteria that did not receive the
plasmid pGLO (-pGLO) were spread onto
agar plates treated with ampicillin (LB-
AMP), incubated for 24 hours at 37˚C and
illuminated with UV light.
If you performed the experiment correctly,
what would you expect to find after
incubation and UV illumination?



Translation is the __?__ part of the central RNA to protein
dogma of molecular biology.

d) all of these answers are correct
Types of mutagens include
a) chemicals
b) infectious agents
c) radiation
d) all of these answers are correct

, 3
During translation, mRNA is read in groups
of bases. How many bases are in each
group?


True/False: False
RNA stands for deoxyribonucleic acid.

3' UCA 5'
A particular triplet of bases in the template
strand of DNA is 5' AGT 3'. The
corresponding codon for the mRNA
transcribed is




Ampicillin kills bacteria
Some of the plates had ampicillin in them,
some did not.
What does ampicillin do to bacteria?

a base deletion near the start of a gene
Which small-scale mutation would be most
likely to have a catastrophic effect on the
functioning of a protein?

Where do germline mutations occur? gametes (sex cells)

During the transcription step of protein mRNA is created by using DNA
synthesis,

initiation
In what step of transcription does RNA
polymerase bind to the promoter on DNA?


d) all of these events are required for a cell to develop into a cancer

What of the following is one of the three
events required for a cell to develop into a
cancer cell?
a) mutation of a proto-oncogene to an
oncogene
b) mutation of a tumor suppressor gene
c) activation of a telomerase gene
d) all of these events are required for a cell
to develop into a cancer cell



False
True/False:
Somatic mutations occur in gametes (sex
cells ie. sperm/egg).

silent
A _______ mutation is a type of point
mutation that codes for the same amino
acid.

If a mother has a mutation in her gametes her child will have the mutation in all of his/her cells

c) both of these answers are correct
Mutations help in evolution because
a) they increase genetic variation
b) they increase the potential for
individuals to differ
c) both of these answers are correct
d) none of these answers are correct


nonsense mutation
Another name for a premature "stop
codon" is a

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Institution
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Course
Freshman Bio

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