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CISSN Exam 2025/2026 – 110+ Practice Questions with Verified Answers | Protein Metabolism, Supplements, Training Nutrition, Hormones & Body Composition

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This CISSN 2025/2026 practice question set features over 110 professionally verified exam-style questions and accurate answers, crafted to reflect the rigor and scope of the Certified Sports Nutritionist (CISSN) exam administered by the International Society of Sports Nutrition (ISSN). The content focuses on critical concepts in exercise metabolism, performance nutrition, and advanced supplementation. Main topics covered: Protein & Amino Acid Metabolism: Leucine signaling, essential vs non-essential amino acids, nitrogen balance, muscle protein synthesis (MPS), TEF Supplementation: Mechanisms and evidence behind creatine, caffeine, beta-alanine, whey protein, omega-3s, and alpha-hydroxy-isocaproic acid Exercise & Recovery Nutrition: Macronutrient distribution, nutrient timing, anabolic windows, post-workout fuel strategies Body Composition & Metabolism: RMR, fat-free mass, thermogenesis, weight loss protocols, ketogenic and high-protein diets Hormonal Influences: Testosterone, cortisol, insulin, thyroid, and their roles in body composition and athletic performance Bioenergetics & Energy Systems: ATP-PC, glycolytic, and oxidative systems, EPOC, TDEE components, and fuel usage during training Micronutrient Support: Vitamin D, magnesium, antioxidants and their relevance to training adaptations Scientific Application & Safety: WADA regulations, side effects of over-supplementation, study design (double-blind, placebo-controlled) Ideal for: CISSN candidates preparing for the 2025/2026 certification exam Sports science, kinesiology, or nutrition students Coaches, dietitians, and strength professionals integrating evidence-based nutrition Competitive athletes and fitness practitioners aiming to optimize body composition and performance This question bank enhances critical thinking and deepens understanding of applied sports nutrition science, supporting both exam success and professional excellence. Keywords: CISSN exam, sports nutrition, ISSN certification, muscle protein synthesis, creatine, caffeine, energy systems, body composition, RMR, thermogenesis, testosterone, fat loss, amino acids, performance nutrition, high-protein diet, supplement science, nutrient timing, BMR, TEF, recovery strategies

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CISSN Questions 2025/2026 Exam
Questions and Answers | A+ Score
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Explain how carnosine is synthesized within the body. - 🧠 ANSWER ✔✔a.

carnosine (beta-alanyl histidine) is made in the body from histidine and beta-

alanine via an energy dependent reaction catalyzed by carnosine synthetase




b.Found in skeletal muscle, cardiac muscle, brain, kidney, and stomach.




c. Available in meats and absorbed in intestine via peptide transporters




d. Antioxidant activity, (hydroxyl and superoxide radical) and oppose

,glycation.




e. Suppress lipid peroxidation and react with protein carbonyl




f. Regulates intracellular calcium and contractility in muscle




g. Primarily, carnosine with pH buffering capacity is widely used in the field of

sports nutrition. Because the dissociation exponent (pKa) of carnosine is 6.83, it is

suggested that carnosine attenuates the reduction in blood pH by a large amount of

H+ originating from the dissociation of lactic acid during strenuous exercise, and

suppresses a loss of force

What is a waxy maize starch and what effect does it have on blood glucose? - 🧠

ANSWER ✔✔a. A highly branched starch called amylopectin. Water soluble and

absorbed by the gut more quickly than dextrose or maltodextrin, research has not

substantiated this claim.

,b. Has been shown to be absorbed with less insulin, allowing ketogenic affects to

remain, increase both glycogen and FFA substrate utilization.

Based on recent studies, which vitamin shows evidence of positively affecting

muscle function? - 🧠 ANSWER ✔✔Vitamin D


Regarding energy drinks (ED), are the following true or false?




The primary ergogenic nutrients in most ED and ES appear to be carbohydrate

and/or caffeine




The ergogenic value of caffeine on mental and physical performance has been

well) established but the potential additive benefits of other nutrients contained in

ED and ES

remains to be determined.




Consuming ED 10-60 minutes before exercise can improve mental focus, alertness,




COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
PRIVACY STATEMENT. ALL RIGHTS RESERVED



3

, anaerobic performance, and/or endurance performance. - 🧠 ANSWER ✔✔The

primary ergogenic nutrients in most ED and ES appear to be carbohydrate and/or

caffeine

True




The ergogenic value of caffeine on mental and physical performance has been

well) established but the potential additive benefits of other nutrients contained in

ED and ES

remains to be determined.

True




Consuming ED 10-60 minutes before exercise can improve mental focus, alertness,

anaerobic performance, and/or endurance performance.

True

What is needed for gluconeogenesis in humans? Give examples of gluconeogenic

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