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CISSN Practice Exam 1 – 2025/2026 | 100+ Verified Q&A | Supplements, Macronutrients, Hydration, Energy Systems, Hormones & Performance Nutrition

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This CISSN Practice Exam 1 (2025/2026) contains over 100 fully verified, high-yield questions and expert-level answers, modeled after the official CISSN certification exam format. It offers a scientifically accurate review of core topics required by the International Society of Sports Nutrition (ISSN), making it ideal for both self-assessment and exam readiness. Key areas covered include: Supplementation Science: Creatine, caffeine, beta-alanine, HMB, betaine, carnosine, whey protein, alpha-hydroxy-isocaproic acid Macronutrients & Energy Metabolism: RER fuel source interpretation, glucose metabolism, fatty acid oxidation, high-protein diets, thermogenesis Muscle Physiology & Protein Synthesis: Contractile proteins, hypertrophy, amino acid utilization, nitrogen metabolism, BCAAs Energy Systems & TDEE Components: BMR, TEF, NEAT, EAT, ATP-PC system, glycolysis, oxidative phosphorylation Hydration & Recovery: Sweat rate variables, electrolyte balance, beer as a post-exercise hydration protocol, overhydration risks Diet & Hormonal Impact: Macronutrient ratios, insulin, anabolic signaling, testosterone, thyroid effects, metabolic adaptation Vitamin & Mineral Functions: Roles of Vitamin D, Vitamin E, magnesium, and their impact on muscle and metabolic performance Clinical & Ethical Aspects: Supplement side effects, placebo response, ergogenic vs banned substances, safety of long-term supplementation Best suited for: CISSN exam candidates aiming for official ISSN certification Sports nutrition students and exercise physiology majors Registered dietitians and performance nutrition coaches Strength and conditioning professionals working with athletes Self-learners wanting to master science-based performance nutrition Every question is precisely explained with real-world application, helping you not only prepare for the exam but also build deep competency in performance-based nutrition and metabolic physiology. Keywords: CISSN exam, ISSN certification, sports nutrition, creatine, caffeine, beta-alanine, energy metabolism, ATP-PC, RER, VO2 max, macronutrients, hydration, muscle physiology, BCAAs, whey protein, fat loss, protein synthesis, supplements, nutrient timing, performance nutrition, high-protein diet

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CISSN Practice Exam 1 2025/2026 Exam
Questions and Answers | 100% Pass



Oral ATP administration can ____ blood flow and may be effective during

exercise _____. - 🧠 ANSWER ✔✔increase, recovery


While running on a treadmill, a subjects RER was measured at 0.75. This indicates

the primary fuel used during this run would be: - 🧠 ANSWER ✔✔free fatty acids


Factors that influence the amount of water lost through sweating include all of the

following except:

- exercise intensity

- relative humidity

- body weight


- environmental temperature - 🧠 ANSWER ✔✔body weight

, In humans, glucose may be produced by gluconeogenesis, synthesizing it from all

of the following except:

- pyruvate

- fatty acids

- glycerol


- carbon skeletons of specific amino acids - 🧠 ANSWER ✔✔fatty acids


_____ exerts the greatest effect on a sedentary person's TDEE - 🧠 ANSWER

✔✔metabolism during rest (RMR)


_____ supplementation can increase muscle power and force output when taken for

14 days at a daily dose of 2.5 grams - 🧠 ANSWER ✔✔Betaine


Cholesterol is needed for which of the following:

- Gluconeogenesis

- Anti-proteolysis


- Steroid hormone synthesis - 🧠 ANSWER ✔✔Steroid hormone synthesis


When an amino acid provides the body with energy, ____ - 🧠 ANSWER ✔✔the

liver must first delaminate it

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