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CISSN Exam 2025/2026 – 120+ Verified Exam Questions & Answers | Macronutrients, Ergogenic Aids, Body Composition, Performance Nutrition & Physiology

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This updated CISSN (Certified Sports Nutritionist) 2025/2026 exam guide delivers 120+ verified multiple-choice questions and answers, meticulously aligned with ISSN’s current examination framework. It is crafted for individuals pursuing certification in sports nutrition science and for professionals needing a robust understanding of exercise performance, supplementation, and metabolism. Core topics thoroughly covered include: Macronutrient Science: Detailed metabolism and role of carbohydrates, fats, and proteins in various training phases Body Composition & Metabolic Health: Fat-free mass, fat oxidation, RMR, thermic effect of food, and hormonal regulation Ergogenic Aids & Supplements: Creatine, caffeine, beta-alanine, HMB, BCAAs, citrulline malate, nitrate, omega-3s, and their effects on performance Hydration & Fluid Balance: Electrolyte function, dehydration, overhydration, and sweat loss adaptations Nutrient Timing & Recovery: Pre-, intra-, and post-exercise nutrition strategies for endurance and resistance athletes Muscle Physiology: Myofibrillar vs sarcoplasmic hypertrophy, satellite cells, fast- vs slow-twitch fibers, muscle protein synthesis Exercise & Clinical Nutrition: VO2 max, lactate threshold, EPOC, insulin sensitivity, energy systems, TDEE Scientific Literacy & Ethics: Double-blind trials, supplement safety, placebo effect, evidence-based nutrition, WADA regulations This resource is ideal for: CISSN certification candidates aiming for ISSN accreditation Students in exercise physiology, sports science, or nutrition degree programs Registered dietitians, personal trainers, strength coaches, and athletic trainers Athletes, fitness professionals, and clinicians applying sports nutrition in real-world settings Whether you're targeting exam success or professional excellence, this document distills essential CISSN concepts into clear, test-ready Q&A form to help you confidently master sports nutrition theory and practice. Keywords: CISSN exam, ISSN certification, sports nutrition, macronutrients, supplements, creatine, caffeine, protein intake, beta-alanine, hydration, nutrient timing, VO2 max, body composition, energy metabolism, ergogenic aids, muscle growth, performance nutrition, electrolyte balance, TDEE, fat oxidation

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CISSN Exam 2025/2026 Exam Questions
and Corresponding Answers with Surety
of 100% Pass Mark



Based on recent studies, which vitamin shows evidence of positively affecting

muscle function? - 🧠 ANSWER ✔✔Vitamin D


Explain how carnosine is synthesized within the body - 🧠 ANSWER ✔✔a.

Carnosine (B-alanyl Histidine) is made in the body from histidine and B-alanine

via an energy dependent reaction catalyzed by carnosine synthetase




b. Found in skeletal muscle, cardiac muscle, brain, kidney, and stomach




c. Available in meats and absorbed in intestine via peptide transporters

,d. Antioxidant activity, (hydroxyl and superoxide radical) and oppose glycation




e. Suppress lipid peroxidation and react with protein carbonyl




f. Regulates intracellular calcium and contractility in muscle




g. Primarily, carnosine with pH buffering capacity is widely used in the field of

sports nutrition. Because the dissociation exponent (pKa) of carnosine is 6.83, it is

suggested that carnosine attenuates the reduction in blood pH by a large amount of

H+ originating from the dissociation of lactic acid during strenuous exercise, and

suppresses a loss of force

What is a waxy maize starch and what effect does it have on blood glucose? - 🧠

ANSWER ✔✔A highly branched starch called amylopectin. Water soluble and

absorbed by the gut more quickly than dextrose or maltodextrin, research has not

substantiated this claim.

,b. Has been shown to be absorbed with less insulin, allowing ketogenic affects to

remain, increase both glycogen and FFA substrate utilization

What is needed for gluconeogenesis in humans? Give examples of gluconeogenic

precursors - 🧠 ANSWER ✔✔Gluconeogenesis relies upon


lactate

pyruvate

glycerol (a catabolic produce of triaglycerols)

certain amino acids (glutamine and alanine

What effect does long-term coffee consumption have on Type II Diabetes? - 🧠

ANSWER ✔✔moderate consumption of both caffeinated and decaffeinated coffee

may lower risk of type 2 diabetes in younger and middle-aged women




Coffee constituents other than caffeine may affect the development of type 2

diabetes.

What effect does supplementing with WPI have on muscle damage? - 🧠 ANSWER

✔✔a. The major finding of this investigation was that whey protein isolate




COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
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3

, supplementation attenuated the impairment in isometric and isokinetic muscle

forces during recovery from exercise-induced muscle injury.




b. The current study demonstrates that whey protein in a partially hydrolysed (pre-

digested) form improves strength recovery rates, possibly due to an increase in the

rate of repair processes and/or a reduction in the extent of damage, from intense

training, in particular, eccentric exercise that is commonly used in weight training.

When glycogen stores are depleted, what macronutrient becomes the predominant

fuel source during distance running? - 🧠 ANSWER ✔✔Fat


How many extra kcals are required for accumulation of 1kg of body fat? - 🧠

ANSWER ✔✔Approximately 7000


What are the effects of pre-exercise dehydration on thermoregulation and

performance - 🧠 ANSWER ✔✔Decreased thermoregulation and performance.


What are the effects of L-alanyl-L-Glutamine supplementation on time-to-

exhaustion and dehydration stress? - 🧠 ANSWER ✔✔AG supplementation provids

a significant ergogenic benefit by:

increasing time to exhaustion during a mild hydration stress.

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