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CISSN Exam 2025/2026 – 100+ Verified Questions & Answers | Macronutrients, Supplements, Muscle Physiology, Hydration, Fat Loss & Sports Nutrition Science

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This CISSN (Certified Sports Nutritionist from the International Society of Sports Nutrition) 2025/2026 exam prep document features over 100 rigorously verified multiple-choice questions and answers, covering the essential scientific and applied principles of sports nutrition. It is specifically designed for candidates aiming to pass the CISSN exam and professionals seeking a deeper understanding of performance nutrition. Key topic areas include: Macronutrient Metabolism: Carbohydrates, fats, and proteins in athletic performance and recovery Supplementation Science: Creatine, caffeine, BCAAs, HMB, beta-alanine, omega-3s, and nitrate usage Body Composition & Weight Management: Fat loss protocols, ketogenic diets, intermittent fasting, energy balance, and metabolic adaptation Muscle Physiology & Hypertrophy: Muscle fiber types, sarcoplasmic vs myofibrillar growth, resistance training adaptations Nutrient Timing: Pre-, intra-, and post-exercise fueling strategies for strength and endurance athletes Hydration & Electrolytes: Sodium, potassium, water balance, sweat rates, and dehydration risks Performance Optimization: VO2 max, lactate threshold, anaerobic capacity, and the role of nutrition in training outcomes Clinical & Ethical Considerations: Drug testing, ergogenic aids, supplementation safety, and evidence-based practice guidelines This study guide is especially useful for: CISSN exam candidates preparing for ISSN certification Nutrition students specializing in sports performance or exercise science Personal trainers, strength coaches, and fitness professionals Health professionals (RDs, MDs, exercise physiologists) working with athletes Competitive athletes and coaches seeking to optimize training through nutrition Every question is crafted to reflect real exam structure and content focus, providing a reliable, science-based preparation experience grounded in current sports nutrition literature and ISSN exam standards. Keywords: CISSN exam, sports nutrition, macronutrients, supplements, creatine, caffeine, muscle growth, hydration, fat loss, nutrient timing, metabolic rate, endurance nutrition, hypertrophy, VO2 max, electrolyte balance, ketogenic diet, protein intake, BCAAs, ergogenic aids, ISSN certification

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CISSN Exam 2025/2026 Exam Questions
and Verified Answers | Already Graded
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If elite runners were broken into two groups and one consumes either a high CHO

diet or a low CHO diet, how would this affect the rate of glycogen breakdown

during running for the two groups? - 🧠 ANSWER ✔✔The rate of glycogen

breakdown would be greater for the high CHO group

What is the main glucose transport protein in the muscles that responds to an

increase insulin level after CHO ingestion? - 🧠 ANSWER ✔✔GLUT 4


When engaging in long durations of exercise (i.e. marathon runner), how long will

it take a marathon runner to recover muscle glycogen if those stores were fully

depleted during a race? - 🧠 ANSWER ✔✔20-24 hours

,Keratin sulfate, hyaluronic acid, chondroitin sulfate are all types of ________. - 🧠

ANSWER ✔✔Glucosaminoglycans


Cellulose, pectin, and legnin are types of ________. - 🧠 ANSWER ✔✔fiber


Insulin is produced by the ________ cell of the _________. - 🧠 ANSWER

✔✔beta-cells, pancreas


During a fasted state, the_______ is able to produce glucose through a process

called _________ and release it into circulation to prevent hypoglycemia. - 🧠

ANSWER ✔✔liver, gluconeogenisis


What stores the most glycogen? - 🧠 ANSWER ✔✔Type II muscles


Carbohydrate digestion (primarily monosaccharides) begins with ________ and

then digestives juices later split the _______ bonds in amylose. - 🧠 ANSWER

✔✔saliva, alpha1-4


What is true about chondroitin sulfate? - 🧠 ANSWER ✔✔it is a

glycosaminoglycan, it is found in joints, it binds to water to maintain fluid

It is recommended for adult males under the age of 50 to consume _______ g of

fiber and adult females under the age of 50 to consume ______ g grams of fiber

per day. - 🧠 ANSWER ✔✔38; 25

,Which of the following is true in regards to the glycemic index? - 🧠 ANSWER

✔✔white bread is the standard food, high glycemic foods will increase glucose

levels to a greater extent at a quicker rate, low glycemic foods will increase blood

glucose levels to a lesser extent and at a slower rate

lactose is a glucose molecule linked to a galactose and is primarily found in milk -

🧠 ANSWER ✔✔true


according to research, 4-5 months of weight training or 8 weeks of cycling training

can increase glycogen stores in the muscle by nearly 36% - 🧠 ANSWER ✔✔true


A reduction in a persons RER as a result from endurance training suggests an

adaptation occurs in the body that allows the muscle to oxidize fatty acids during

exercise - 🧠 ANSWER ✔✔true


the ideal CHO sport drink would contain _______ % CHO and athletes should

drink this every ______ hour of endurance performance. - 🧠 ANSWER ✔✔6-8%;

one


What is true regarding glycogen loading? - 🧠 ANSWER ✔✔it is also known as

carb loading, it is used to enhance performance for when the time is greater than 90

minutes, it can increase glycogen stores 50%, the classic method involves

depleting muscle glycogen stores for 3 days and then loading for 3 days

COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
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3

, What would be recommended to minimize protein degradation? - 🧠 ANSWER

✔✔consume CHO and protein immediately after exercise


Glycogen depletion during exercise is associated with which of the following? - 🧠

ANSWER ✔✔muscular fatigue and hypoglycemia


athletes need to experiment with different pre, during, and post CHO intake

protocols in the days leading up to exercise so they can fuel optimally for their

competition - 🧠 ANSWER ✔✔true


What is true regarding the BCAA's leucine, isoleucine, and valine? - 🧠 ANSWER

✔✔they are unique among other EAAs for their roles in protein metabolism as

well as the ability to reduces DOMS

Findings suggest that the faster digesting ______ protein is more beneficial for

skeletal muscle adaptations than the slower digesting _______ protein. - 🧠

ANSWER ✔✔whey; casein


After a bout of high intensity exercise that lasts for 60 minutes, in order to achieve

maximal protein synthesis, it would be optimal for the athlete to - 🧠 ANSWER

✔✔drink whey protein combined with milk

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