MB (ASCP) Certification UPDATED
ACTUAL Exam Questions and CORRECT
Answers
If DNA has an A260/280 value <1.7 then - CORRECT ANSWER - too much protein or
phenol
If RNA has an A260/280 value <1.7 then - CORRECT ANSWER - guanidine thiocyanate
or B-mercaptoethanol contamination
guanidine thiocyanate - CORRECT ANSWER - Used in extractions. It can denature
Rnases and Dnases
How does a fluorometer measure fluorescence related to DNA concentration? - CORRECT
ANSWER - utilizes a Hoechst dye that is highly specific to DNA, binding to Adenine-
Thymine (A-T) rich regions of the minor groove of double stranded DNA
Pol α: - CORRECT ANSWER - forms complex with pol subunits to act as a primase
(synthesizing an RNA primer) then elongates that primer for ~20 nts
Pol β: - CORRECT ANSWER - DNA repair (base-excision and gap-filling)
Pol γ: - CORRECT ANSWER - replicates and repairs mitochondrial DNA
Pol δ: - CORRECT ANSWER - exonuclease, plus might be main one involved in lagging
strand synthesis
Pol ε: - CORRECT ANSWER - exonuclease, plus main one involved in leading strand
synthesis, maybe
, Pol λ and μ: - CORRECT ANSWER - non-homologous end-joining repair
Pol η, ι, κ, ζ: - CORRECT ANSWER - not really good and will replicate through damaged
DNA
RNA polymerase I: - CORRECT ANSWER - 5.8S, 18S, and 28S rRNA genes
RNA polymerase II: - CORRECT ANSWER - all protein-coding genes, plus snoRNA
genes, miRNA genes, siRNA genes, and most snRNA genes
RNA polymerase III: - CORRECT ANSWER - tRNA genes, 5S rRNA genes, some
snRNA genes, and genes for other small RNAs
What catalyzes polymerization of polyacrylamide? - CORRECT ANSWER - APS and
TEMED
high-resolution banding - CORRECT ANSWER - staining chromosomes before they reach
maximal metaphase condensation to increase visualization of bands
G or Q banding - CORRECT ANSWER - Heterochromatin stains dark
R banding - CORRECT ANSWER - Euchromatin stains darkly
C banding - CORRECT ANSWER - Stains only the centromeres
Fluorescence In Situ Hybridization (FISH) - CORRECT ANSWER - fluorescent probes
hybridize to sequences in their natural chromosome positions to determine the positions of genes
Colcemid - CORRECT ANSWER - stops dividing cells at metaphase
ACTUAL Exam Questions and CORRECT
Answers
If DNA has an A260/280 value <1.7 then - CORRECT ANSWER - too much protein or
phenol
If RNA has an A260/280 value <1.7 then - CORRECT ANSWER - guanidine thiocyanate
or B-mercaptoethanol contamination
guanidine thiocyanate - CORRECT ANSWER - Used in extractions. It can denature
Rnases and Dnases
How does a fluorometer measure fluorescence related to DNA concentration? - CORRECT
ANSWER - utilizes a Hoechst dye that is highly specific to DNA, binding to Adenine-
Thymine (A-T) rich regions of the minor groove of double stranded DNA
Pol α: - CORRECT ANSWER - forms complex with pol subunits to act as a primase
(synthesizing an RNA primer) then elongates that primer for ~20 nts
Pol β: - CORRECT ANSWER - DNA repair (base-excision and gap-filling)
Pol γ: - CORRECT ANSWER - replicates and repairs mitochondrial DNA
Pol δ: - CORRECT ANSWER - exonuclease, plus might be main one involved in lagging
strand synthesis
Pol ε: - CORRECT ANSWER - exonuclease, plus main one involved in leading strand
synthesis, maybe
, Pol λ and μ: - CORRECT ANSWER - non-homologous end-joining repair
Pol η, ι, κ, ζ: - CORRECT ANSWER - not really good and will replicate through damaged
DNA
RNA polymerase I: - CORRECT ANSWER - 5.8S, 18S, and 28S rRNA genes
RNA polymerase II: - CORRECT ANSWER - all protein-coding genes, plus snoRNA
genes, miRNA genes, siRNA genes, and most snRNA genes
RNA polymerase III: - CORRECT ANSWER - tRNA genes, 5S rRNA genes, some
snRNA genes, and genes for other small RNAs
What catalyzes polymerization of polyacrylamide? - CORRECT ANSWER - APS and
TEMED
high-resolution banding - CORRECT ANSWER - staining chromosomes before they reach
maximal metaphase condensation to increase visualization of bands
G or Q banding - CORRECT ANSWER - Heterochromatin stains dark
R banding - CORRECT ANSWER - Euchromatin stains darkly
C banding - CORRECT ANSWER - Stains only the centromeres
Fluorescence In Situ Hybridization (FISH) - CORRECT ANSWER - fluorescent probes
hybridize to sequences in their natural chromosome positions to determine the positions of genes
Colcemid - CORRECT ANSWER - stops dividing cells at metaphase
