DISCOVERY LAB EXAM JEOPARDY
EXAM QUESTIONS AND ANSWERS
Criteria used to determine a "hit" in the compound library - ANSWER-the mean of the
DMSO-treated samples +/- two standard deviations
The concentration of 10 uM of compound after a 1:10 dilution into the media -
ANSWER-1 uM
Source of the compounds we are testing in the compound screen - ANSWER-National
Cancer Institute
Term that describes the dose at which 50% of the Salmonella will die - ANSWER-ED50
Term that describes the dose at which 50% of off-target cells will die - ANSWER-TD50
Type of screen that involves measuring an observable trait after treatment with a
compound - ANSWER-phenotypic screen
The value that compares the dose of a compound that produces a therapeutic effect
and to the dose that produces a toxic effect - ANSWER-the therapeutic index
Experiments that take place "in glass" - ANSWER-in vitro
The parts of a compound structure that confers biological properties - ANSWER-a
functional group
Term that describes structurally similar compounds - ANSWER-analogs
Term that describes the relationship between the shape of the compound and it's
biological function - ANSWER-structure-activity relationship (SAR)
Type of variable represented by death of Salmonella - ANSWER-the dependent variable
in our experiments
Type of variable represented by our compounds - ANSWER-independent variables in
our experiment
The lack of knowledge of the identity of compounds when setting up an experiment -
ANSWER-blinding
The disease phenotype caused by Salmonella Typhimurium in mice - ANSWER-typhoid
fever
The positive control in our compound screen - ANSWER-Ampicillin
EXAM QUESTIONS AND ANSWERS
Criteria used to determine a "hit" in the compound library - ANSWER-the mean of the
DMSO-treated samples +/- two standard deviations
The concentration of 10 uM of compound after a 1:10 dilution into the media -
ANSWER-1 uM
Source of the compounds we are testing in the compound screen - ANSWER-National
Cancer Institute
Term that describes the dose at which 50% of the Salmonella will die - ANSWER-ED50
Term that describes the dose at which 50% of off-target cells will die - ANSWER-TD50
Type of screen that involves measuring an observable trait after treatment with a
compound - ANSWER-phenotypic screen
The value that compares the dose of a compound that produces a therapeutic effect
and to the dose that produces a toxic effect - ANSWER-the therapeutic index
Experiments that take place "in glass" - ANSWER-in vitro
The parts of a compound structure that confers biological properties - ANSWER-a
functional group
Term that describes structurally similar compounds - ANSWER-analogs
Term that describes the relationship between the shape of the compound and it's
biological function - ANSWER-structure-activity relationship (SAR)
Type of variable represented by death of Salmonella - ANSWER-the dependent variable
in our experiments
Type of variable represented by our compounds - ANSWER-independent variables in
our experiment
The lack of knowledge of the identity of compounds when setting up an experiment -
ANSWER-blinding
The disease phenotype caused by Salmonella Typhimurium in mice - ANSWER-typhoid
fever
The positive control in our compound screen - ANSWER-Ampicillin
