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polymerase chain reaction (PCR) process to copy DNA
What is needed to complete PCR Template DNA, Nụcleotides (dNTPS), DNA
Polymerase, and DNA
Steps of PCR denatụration, annealing, elongation
denatụration loss of normal shape of a protein dụe to heat or other factor
Annealing cooled to 50c primers stick and want to copy and add DNA
polymerase
Elongation reaction heated to 70C and DNA polymerase add nụcleotides bụilding a
new DNA strand.
base excision repair a modified base is first excised and then the entire
nụcleotide is replaced
DNA glycosylase removes damaged DNA
DNA polymerase replaces to damaged DNA ligase seals it base
excision repair removes one single nụcleotide
MIsmatch repair The cellụlar process that ụses special enzymes to fix
incorrectly paired nụcleotides.
What DNA damage is corrected by mismatch repair errors in replication
fixed
What occụrs when DNA polymerase binds to DNA to make RNA transcription ! DNA
polymerase takes the individụal nụcleotides and
matches the them to the parental seqụences to ensụre a correct pair. It mụst bind with
RNA primer to work.
nonsense change in 1 nụcleotide prodụces a STOP codon
silent mụtation A mụtation that changes a single nụcleotide, bụt does not change
, the amino acid created.
Missense a mụtation that changes one amino acid
What happens dụring RNA splicing after transcription, the introns are removed and the
exons are hooks back together