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BIOL 438 Exam #1 2025/2026 Exam Questions and Answers 100% Guaranteed Success | Already Rated A+ After this Gram stain, both Gram-positive bacteria and Gram-negative bacteria are purple. -

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BIOL 438 Exam #1 2025/2026 Exam
Questions and Answers 100%
1
Guaranteed Success | Already Rated A+




After this Gram stain, both Gram-positive bacteria and Gram-negative bacteria are

purple. - 🧠 ANSWER ✔✔Crystal Violet


After this Gram stain step, purple Gram-positive bacteria and purple Gram-

negative bacteria remain purple. - 🧠 ANSWER ✔✔Gram's Iodine


After this Gram stain step, Gram-positive bacteria are purple and Gram-negative

bacteria are red or pink. - 🧠 ANSWER ✔✔Safranin


After this Gram stain step, Gram-positive bacteria are purple and Gram-negative

bacteria are colorless. - 🧠 ANSWER ✔✔Alcohol Decolorizer


Which microscope would be the best to use to view components within a cell? - 🧠

ANSWER ✔✔Transmission Electron Microscope




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,The most commonly used type of microscopy is called ___________ , because the

specimens produce dark images against brighter backgrounds. - 🧠 ANSWER
2
✔✔bright-field


In ______________ microscopy, however, bright images on a darker background

are produced because only light that is refracted by the specimen is captured. - 🧠

ANSWER ✔✔dark-field


The ____________ microscope uses slight differences in refractive indices and an

annular condenser to produce sharper images than simple bright-field microscopy.

- 🧠 ANSWER ✔✔phase-contrast


When polarized light is needed for imaging a ___________ microscope is used. - 🧠

ANSWER ✔✔differential interference contrast


Light at specific wavelengths is used to excite dyes in specimens that then emit

light at specific wavelengths in ____________ - 🧠 ANSWER ✔✔epifluorescence


Laser light is used in _____________, generating three-dimensional images. - 🧠

ANSWER ✔✔confocal


How does the addition of immersion oil provide better magnification than a high

power lens alone? - 🧠 ANSWER ✔✔The immersion oil has the same refractive

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, index as glass; by placing a drop of oil between the glass slide and the lens, this

eliminates two refractive surfaces.
3
Select all that are characteristics of transmission electron microscopes. - 🧠

ANSWER ✔✔-Highest practical magnification over 100,000 times.


-Uses electron beam for radiation source.

-Adjust current to magnetic lenses to change magnification.

What microscope would be the best to use to view cell surface features of a

specimen? - 🧠 ANSWER ✔✔Scanning


Which of the following stains would best determine if a bacterium has a single cell

membrane plus a very thick cell wall or if a bacterium has a very thin cell wall in

between two cell membranes? - 🧠 ANSWER ✔✔Gram Stain


Put the following steps in order to review the bacterial method of iron uptake for

the cell. - 🧠 ANSWER ✔✔1. Iron scarce in environment


2.Bacteria secretes siderophore

3. Iron-siderophore complex binds cell surface protein

4. Iron-siderophore complex enters cell via ABC transporter



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