CG(ASCP) EXAM QUESTIONS AND ANSWERS (VERIFIED AND WELL
DETAILED ANSWERS) LATEST 2025/2026
The most important reason why biological safety cabinets are used instead of
horizontal laminar flow hoods in the handling of human tissues is
to protect the technologist who is processing the specimens
In which of the following situations would one suspect bacterial contamination
culture media is turbid in appearance
Which of the following chemicals is used for cryopreservation?
dimethyl sulfoxide (DMSD)
Which of the following is a buffer used in cultures?
HEPES
In order for the fragile X to be expressed in affected individuals, lymphocytes
must be grown in what type of media?
folic acid deficient
The following enzymes cab be used to dissociate Chronic Villus samples (CVS)
except for
RNAse
Which of the following is not a necessary ingredient which is added to
modified medium?
antibiotics
All of the following are factors which affect the cryopreservation except
A fast growing culture shows signs of cell death. What is a possible reason for
this?
cell confluency: too many or too few cells
,collagenase, pronase, and trypsin are used to disperse tissue into single-cell
suspension. These substances are:
proteolytic enzymes
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with:
colcemid
Which of the following culture processes would be most useful for identifying
patients w/ fanconi anemia?
mitomycin-C stress test of peripheral blood
To avoid potential culture failure, it is important to monitor the reagents used.
Recording which of the following information will be most important when
documenting culture failure?
expiration date
The morning after being set-up, a single dish of amniotic fluid culture turns
cloudy and appears contaminated. None of the other cultures set up that day
appear contaminated. Which of the following should be done first?
harvest the culture as soon as possible
If an amniotic fluid culture from the same sample grows well in a different
incubator, the probable cause of the culture failure is
incubator failure
Positive staining w/ Hoechst 33258 reveals micronuclei. Which of the following
agents caused the culture contamination?
mycoplasma
Which of the following statements is true about trypsin>
is is useful in removing cells from culture flasks
Which of the following is the best tissue to use for both a good success rate and
abnormality detection in spontaneous abortion specimen
, chorionic villi
When a culture becomes contaminated, the culture medium shifts to an acidic
pH. If the medium contains a pH indicator, the color of the medium in the
flask will appear
yellow
Which of the following reagents is used in culturing peripheral blood, but not
in culturing bone marrow or amniotic fluid or chromosome analysis?
phytohemagglutinin (PHA)
Which of the following is the best agent to use for the presence of mycoplasma
growth in a culture?
BM cycline
A bone marrow culture on a patient w/ APL M3 has failed to yield analyzable
metaphases. The culture had a cell density of 1x10^4 calls/ml. The specimen
had been in transit for 2 days and was not sent in a transport medium. The
most likely cause of the failure is:
too much time in transit
Which of the following microscopes should be used when cleaning a chorionic
villus specimen?
dissection
Blood specimen for chromosome analysis may be kept in culture up to how
many hours after culture initiation?
96 hours
which of the following cell types is diagnostically most problematic if present
in a CVS sample?
maternal decidua
Cells from a skin fibroblast culture are to be frozen for storage in liquid
nitrogen. The cell concentration is 2X10^7 cells/ml. How many 2ml vials can
be frozen if the final cell concentration for storage is to be 2X10^6?
DETAILED ANSWERS) LATEST 2025/2026
The most important reason why biological safety cabinets are used instead of
horizontal laminar flow hoods in the handling of human tissues is
to protect the technologist who is processing the specimens
In which of the following situations would one suspect bacterial contamination
culture media is turbid in appearance
Which of the following chemicals is used for cryopreservation?
dimethyl sulfoxide (DMSD)
Which of the following is a buffer used in cultures?
HEPES
In order for the fragile X to be expressed in affected individuals, lymphocytes
must be grown in what type of media?
folic acid deficient
The following enzymes cab be used to dissociate Chronic Villus samples (CVS)
except for
RNAse
Which of the following is not a necessary ingredient which is added to
modified medium?
antibiotics
All of the following are factors which affect the cryopreservation except
A fast growing culture shows signs of cell death. What is a possible reason for
this?
cell confluency: too many or too few cells
,collagenase, pronase, and trypsin are used to disperse tissue into single-cell
suspension. These substances are:
proteolytic enzymes
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with:
colcemid
Which of the following culture processes would be most useful for identifying
patients w/ fanconi anemia?
mitomycin-C stress test of peripheral blood
To avoid potential culture failure, it is important to monitor the reagents used.
Recording which of the following information will be most important when
documenting culture failure?
expiration date
The morning after being set-up, a single dish of amniotic fluid culture turns
cloudy and appears contaminated. None of the other cultures set up that day
appear contaminated. Which of the following should be done first?
harvest the culture as soon as possible
If an amniotic fluid culture from the same sample grows well in a different
incubator, the probable cause of the culture failure is
incubator failure
Positive staining w/ Hoechst 33258 reveals micronuclei. Which of the following
agents caused the culture contamination?
mycoplasma
Which of the following statements is true about trypsin>
is is useful in removing cells from culture flasks
Which of the following is the best tissue to use for both a good success rate and
abnormality detection in spontaneous abortion specimen
, chorionic villi
When a culture becomes contaminated, the culture medium shifts to an acidic
pH. If the medium contains a pH indicator, the color of the medium in the
flask will appear
yellow
Which of the following reagents is used in culturing peripheral blood, but not
in culturing bone marrow or amniotic fluid or chromosome analysis?
phytohemagglutinin (PHA)
Which of the following is the best agent to use for the presence of mycoplasma
growth in a culture?
BM cycline
A bone marrow culture on a patient w/ APL M3 has failed to yield analyzable
metaphases. The culture had a cell density of 1x10^4 calls/ml. The specimen
had been in transit for 2 days and was not sent in a transport medium. The
most likely cause of the failure is:
too much time in transit
Which of the following microscopes should be used when cleaning a chorionic
villus specimen?
dissection
Blood specimen for chromosome analysis may be kept in culture up to how
many hours after culture initiation?
96 hours
which of the following cell types is diagnostically most problematic if present
in a CVS sample?
maternal decidua
Cells from a skin fibroblast culture are to be frozen for storage in liquid
nitrogen. The cell concentration is 2X10^7 cells/ml. How many 2ml vials can
be frozen if the final cell concentration for storage is to be 2X10^6?
