MBIO exam 2 Questions and Answers Fully Solved Graded A+
1. In a restaurant, which decontamination procedure includes both antisepsis and degermation?
A. After handling raw meat, a cook washes his hands with soap containing triclosan
B. After handling raw meat, a cook wipes down the preparation surface with a diluted bleach solution
C. Meat is cooked to an internal temperature of 63°C to kill potential foodborne pathogens
D. Raw meat is placed in a sealed container in the refrigerator to prevent spoilage
E. Utensils and glassware are cleaned in a dishwasher with hot water and detergent - Answers After
handling raw meat, a cook washes his hands with soap containing triclosan
Opportunistic infection infections are most likely to occur in all of the following hosts except:
A. Cystic fibrosis (CF) patient
B. Healthcare worker
C. Individual with AIDS
D. Newborn
E. Severe burn wound patient - Answers B. Healthcare worker
1. A patient being treated with a broad-spectrum antibiotic develops a yeast infection caused by
Candida albicans, which is a type of __________ that is caused by the depletion of normal flora bacteria.
A. Epidemic
B. Antibiotic resistance
C. Septic shock
D. Superinfection
E. Zoonosis - Answers D. Superinfection
1. What is the target of isoniazid (INH), which is used to treat tuberculosis (TB)?
A. Lipid synthesis
B. Mycolic acid synthesis
,C. Nucleic acid synthesis
D. Peptidoglycan synthesis
E. Protein synthesis - Answers . Mycolic acid synthesis
1. In which location does the greatest number of normal flora bacteria exist in humans?
A. Blood
B. Large intestine
C. Skin
D. Stomach
E. Urinary bladder - Answers B. Large intestine
1. While studying Staphylococcus aureus in 1928, Scottish doctor Alexander Fleming discovered
penicillin, the first natural antibiotic compound, which was being produced by a common bread mold.
What phenomenon did he observe?
A. Antibiotic resistance
B. Chain of transmission
C. Microbial antagonism
D. Natural selection
E. Superinfection - Answers C. Microbial antagonism
1. Which method can be used continuously (24 hours-a-day) to eliminate airborne microbes from the
atmosphere in a hospital "clean" room used for patient surgeries?
A. HEPA filtration
B. Ionizing radiation
C. Lyophilization
D. Non-ionizing radiation
E. Tyndallization - Answers A. HEPA filtration
1. Which of the following describes indirect contact transmission via fomites?
A. A man contracts cryptosporidiosis from ingesting chlorine-resistant cysts while swimming in a public
pool.
,B. A nurse touches the bedrail in a MRSA patient's room and unknowingly spreads the bacteria to two
other patients because she did not wash her hands.
C. A person walks barefoot on soil contaminated with feces and becomes infected with a hookworm
that burrows under the skin on his toes.
D. A prostitute increases her risk of becoming infected with syphilis by having unprotected sex with
many clients.
E. After a house became flooded during a storm, black mold began to grow, which sickened the house's
residents who inhaled the airborne spores. - Answers B. A nurse touches the bedrail in a MRSA patient's
room and unknowingly spreads the bacteria to two other patients because she did not wash her hands.
1. When T4 bacteriophage infect a layer of E. coli cells growing on an agar plate, visible areas of lysis
called __________ will appear.
A. Plaques
B. Colonies
C. Capsules
D. Prophage - Answers A. Plaques
1. Beta-lactam antibiotics bind to bacterial transpeptidase and inhibit:
A. Formation of crosslinks in cell wall
B. Formations of glycan chains in cell wall
C. Inactivation of penicillinase
D. Insertion of ergosterol in cell membrane
E. Insertion of mycolic acids in cell wall - Answers Formation of crosslinks in cell wall
1. Which statement about bacterial endotoxin is FALSE?
A. Endotoxin causes systemic responses including inflammation, fever, and intravascular coagulation.
B. Endotoxin is a molecule called lipopolysaccharide.
C. Endotoxin is embedded in the bacterial outer membrane.
D. Endotoxin is actively secreted by bacteria during infection.
E. Endotoxin is only produced by gram-negative bacteria. - Answers D. Endotoxin is actively secreted by
bacteria during infection.
, 1. Which combination therapy is intended to suppress the development of resistance and includes
antibiotics that are used in nonprescription ointment?
A. Amoxicillin & clavulanate
B. Azidothymidine (AZT) & saquinavir
C. Bacitracin, neomycin, & polymyxin B
D. Isoniazid (INH) & rifampin
E. Sulfamethoxazole & trimethoprim (SXT) - Answers C. Bacitracin, neomycin, & polymyxin B
1. Most viral vaccines are heat-sensitive and will be inactivated by warm temperatures. What method
would NOT be used for preventing bacterial growth and contamination in a viral vaccine?
A. Filtration
B. Freezing
C. Lyophilization
D. Pasteurization
E. Refrigeration - Answers D. Pasteurization
1. In 1854, John Snow convinced city officials to remove the Broad Street water pump handle to stop an
outbreak of cholera caused by contamination by human waste. How did he determine the origin of the
infection?
A. He tested each of the infected people for the cholera bacteria
B. He tested each of the water pumps and wells for the cholera bacteria
C. He plotted all of the cases of disease on a map and looked for where they were most concentrated
D. He took the water pump handles off each of the city's wells until the outbreak stopped
E. Just a lucky guess - Answers A. He plotted all of the cases of disease on a map and looked for where
they were most concentrated
1. A surgical patient develops a MRSA infection at the incision site. It is determined that a healthcare
worker picked up the MRSA from another patient and then transferred the bacteria while changing the
patient's dressing. Which term is not associated with this infection?
A. Endogenous infection
B. Nosocomial infection
1. In a restaurant, which decontamination procedure includes both antisepsis and degermation?
A. After handling raw meat, a cook washes his hands with soap containing triclosan
B. After handling raw meat, a cook wipes down the preparation surface with a diluted bleach solution
C. Meat is cooked to an internal temperature of 63°C to kill potential foodborne pathogens
D. Raw meat is placed in a sealed container in the refrigerator to prevent spoilage
E. Utensils and glassware are cleaned in a dishwasher with hot water and detergent - Answers After
handling raw meat, a cook washes his hands with soap containing triclosan
Opportunistic infection infections are most likely to occur in all of the following hosts except:
A. Cystic fibrosis (CF) patient
B. Healthcare worker
C. Individual with AIDS
D. Newborn
E. Severe burn wound patient - Answers B. Healthcare worker
1. A patient being treated with a broad-spectrum antibiotic develops a yeast infection caused by
Candida albicans, which is a type of __________ that is caused by the depletion of normal flora bacteria.
A. Epidemic
B. Antibiotic resistance
C. Septic shock
D. Superinfection
E. Zoonosis - Answers D. Superinfection
1. What is the target of isoniazid (INH), which is used to treat tuberculosis (TB)?
A. Lipid synthesis
B. Mycolic acid synthesis
,C. Nucleic acid synthesis
D. Peptidoglycan synthesis
E. Protein synthesis - Answers . Mycolic acid synthesis
1. In which location does the greatest number of normal flora bacteria exist in humans?
A. Blood
B. Large intestine
C. Skin
D. Stomach
E. Urinary bladder - Answers B. Large intestine
1. While studying Staphylococcus aureus in 1928, Scottish doctor Alexander Fleming discovered
penicillin, the first natural antibiotic compound, which was being produced by a common bread mold.
What phenomenon did he observe?
A. Antibiotic resistance
B. Chain of transmission
C. Microbial antagonism
D. Natural selection
E. Superinfection - Answers C. Microbial antagonism
1. Which method can be used continuously (24 hours-a-day) to eliminate airborne microbes from the
atmosphere in a hospital "clean" room used for patient surgeries?
A. HEPA filtration
B. Ionizing radiation
C. Lyophilization
D. Non-ionizing radiation
E. Tyndallization - Answers A. HEPA filtration
1. Which of the following describes indirect contact transmission via fomites?
A. A man contracts cryptosporidiosis from ingesting chlorine-resistant cysts while swimming in a public
pool.
,B. A nurse touches the bedrail in a MRSA patient's room and unknowingly spreads the bacteria to two
other patients because she did not wash her hands.
C. A person walks barefoot on soil contaminated with feces and becomes infected with a hookworm
that burrows under the skin on his toes.
D. A prostitute increases her risk of becoming infected with syphilis by having unprotected sex with
many clients.
E. After a house became flooded during a storm, black mold began to grow, which sickened the house's
residents who inhaled the airborne spores. - Answers B. A nurse touches the bedrail in a MRSA patient's
room and unknowingly spreads the bacteria to two other patients because she did not wash her hands.
1. When T4 bacteriophage infect a layer of E. coli cells growing on an agar plate, visible areas of lysis
called __________ will appear.
A. Plaques
B. Colonies
C. Capsules
D. Prophage - Answers A. Plaques
1. Beta-lactam antibiotics bind to bacterial transpeptidase and inhibit:
A. Formation of crosslinks in cell wall
B. Formations of glycan chains in cell wall
C. Inactivation of penicillinase
D. Insertion of ergosterol in cell membrane
E. Insertion of mycolic acids in cell wall - Answers Formation of crosslinks in cell wall
1. Which statement about bacterial endotoxin is FALSE?
A. Endotoxin causes systemic responses including inflammation, fever, and intravascular coagulation.
B. Endotoxin is a molecule called lipopolysaccharide.
C. Endotoxin is embedded in the bacterial outer membrane.
D. Endotoxin is actively secreted by bacteria during infection.
E. Endotoxin is only produced by gram-negative bacteria. - Answers D. Endotoxin is actively secreted by
bacteria during infection.
, 1. Which combination therapy is intended to suppress the development of resistance and includes
antibiotics that are used in nonprescription ointment?
A. Amoxicillin & clavulanate
B. Azidothymidine (AZT) & saquinavir
C. Bacitracin, neomycin, & polymyxin B
D. Isoniazid (INH) & rifampin
E. Sulfamethoxazole & trimethoprim (SXT) - Answers C. Bacitracin, neomycin, & polymyxin B
1. Most viral vaccines are heat-sensitive and will be inactivated by warm temperatures. What method
would NOT be used for preventing bacterial growth and contamination in a viral vaccine?
A. Filtration
B. Freezing
C. Lyophilization
D. Pasteurization
E. Refrigeration - Answers D. Pasteurization
1. In 1854, John Snow convinced city officials to remove the Broad Street water pump handle to stop an
outbreak of cholera caused by contamination by human waste. How did he determine the origin of the
infection?
A. He tested each of the infected people for the cholera bacteria
B. He tested each of the water pumps and wells for the cholera bacteria
C. He plotted all of the cases of disease on a map and looked for where they were most concentrated
D. He took the water pump handles off each of the city's wells until the outbreak stopped
E. Just a lucky guess - Answers A. He plotted all of the cases of disease on a map and looked for where
they were most concentrated
1. A surgical patient develops a MRSA infection at the incision site. It is determined that a healthcare
worker picked up the MRSA from another patient and then transferred the bacteria while changing the
patient's dressing. Which term is not associated with this infection?
A. Endogenous infection
B. Nosocomial infection
