Function: Quiz 1 Exam
Questions with Correct
Answers
How do you inoculate a petri dish? - Correct Answer: 1) Touch sterile swab to sample surface, then rub
swab across the petri dish
2) Label bottom of petri dish and leave it upside down
Why should Petri dishes be stored upside down? - Correct Answer: to prevent condensation from
dropping on media and contaminating sample
What are asceptic techniques? - Correct Answer: 1) wash hands before doing lab work
2) wear gloves
3) use disinfectant to clean work area before and after lab work
4) always keep lids on petri dishes
5) don't touch the sterile swab to any surface except sample
6) properly dispose of waste when finished
What are the characteristics of yeast? - Correct Answer: mound colonies
less transparent
what are the characteristics of mold - Correct Answer: very large
fuzzy
dark
what are the characteristics of bacteria? - Correct Answer: medium colonies
yellow/not transparent
, This identifies closest to .. - Correct Answer:
What is the first stop for in pipettes? - Correct Answer: to load correct volume
what is second stop for in pipettes? - Correct Answer: to eject all the liquid
range of p20 - Correct Answer: 2microliters-20microliters
range of p200 - Correct Answer: 20microliters-200microliters
range of p1000 - Correct Answer: 200microliters-1000microliters
different types of bacterial diversity - Correct Answer: 1) gram+/-
2) shape (color)
3) metabolism
4) capability of bacteria to live in extreme environment
5) cellular defense
What distinguishes gram-positive bacteria? - Correct Answer: -thick layer of peptidoglycan
- absence of outer membrane of lipopolysaccharides
What distinguishes gram-negative bacteria? - Correct Answer: - thinner peptidoglycan layer
- presence of outer membrane lipopolysaccharides
What are 16S ribosomal RNA (rRNA) important for? - Correct Answer: protein synthesis
Why are 16S ribosomal RNA considered functionally constrained? - Correct Answer: mutations in 16S
rRNA sequence are likely to be deleterious