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BIOD 171 Microbiology Module 4 Exam | 2025 Actual Exam | Portage Learning, Exams of Biology

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Exam covering microbial growth media types (selective, differential, enriched), specific agar formulations (Blood, MacConkey, EMB, MSA, CNA, Chocolate), hemolysis identification, plating methods (quadrant streak), pure culture concepts, and bacterial growth requirements.

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BIOD 171 Microbiology Module 4 Exam |
2025 Actual Exam | Portage Learning, Exams
of Biology



T/F: Growth media is best suited for distinguishing between tw
c c c c c c c c c




o similar species of bacteria ......answer.....False
c c c c cc




growth media is designed to simply support (and not restrict)
c c c c c c c c c c




microbial growth c

,A researcher is asked to determine which of two vials contains
c c c c c c c c c c c




E coli and which contains salmonella. Knowing both are Gram-
c c c c c c c c c




negative while only one of them is capable of fermenting lactos
c c c c c c c c c c




e, which type of media would be best suited? ......answer.....Diff
c c c c c c c c cc




erential media c




What are the requirements of a fastidious microbe? ......answer
c c c c c c c cc




.....A fastidious microbe is an organism with complex growth re
c c c c c c c c c




quirements and will not grow without then. Enriched medias t
c c c c c c c c c




hus contain these specific and essential nutrients required for t
c c c c c c c c c




he growth of a particular subset of microorganisms.
c c c c c c c




T/F: LB agar is classified as a non-selective, non-
c c c c c c c c




differential media ......answer.....True c cc

,The polysaccharide-
c




based hardening agent added to LB media to produce LB agar is
c c c c c c c c c c c c




derived from what? ......answer.....Seaweed extract
c c cc c




Blood agar is which type of medium? Select all that apply:
c c c c c c c c c c




- Selective and Differential
c c c




- Enriched
c




- Differential
c




- Selective ......answer.....- Enriched
c cc c




- Differential
c




Match the following hemolytic class with its description of acti
c c c c c c c c c




vity: c




1. Alpha hemolysis
c c

, 2. Beta hemolysis
c c




3. Gamma hemolysis
c c




A. No change
c c




B. Greenish-brown color
c c




C. Distinct zone of clearing ......answer.....1. Alpha hemolysis-
c c c c cc c c




Greenish-brown color (B)
c c c




2. Beta hemolysis- Distinct zone of clearing (C)
c c c c c c c




3. Gamma hemolysis- No change (A)
c c c c c




Match the following hemolytic class with its description of acti
c c c c c c c c c




vity: c




1. Alpha hemolysis
c c

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