UAMS Microbiology exam 3 UPDATED
ACTUAL Exam Questions and CORRECT
Answers
Nonfermentors vs enterics - CORRECT ANSWER - -Oxidase positive
-OF glucose test
-Colorless growth on MAC
Nonfermentors - CORRECT ANSWER - MO's that cant metabolize sugars in anaerobic
conditions
Fermentor - CORRECT ANSWER - Utilize carbohydrates for energy both aerobically and
anaerobically
Oxidizer - CORRECT ANSWER - Metabolizes carbohydrates in ari
Non-oxidizer - CORRECT ANSWER - Cannot utilize sugar as an energy source
Oxidative-Fermentative Test - CORRECT ANSWER - -Determines if a MO is a
fermentor, oxidizer or non-oxidizer
***Make sure to test each MO aerobically and anaerobically***
Oxidative-Fermentative Test (Nonfermentor, non oxidizer) - CORRECT ANSWER -
Describe the reaction for tube set one
Oxidative-Fermentative Test (Nonfermentor, oxidizer) - CORRECT ANSWER - Describe
the reaction for tube set two
,Oxidative-Fermentative Test (Fermentor, oxidizer) - CORRECT ANSWER - Describe the
reaction for tube set three
Nitrate reduction test - CORRECT ANSWER - -Determine in MO contains nitrate
reductase (convert nitrate -->nitrite)
-Positive = red
-Negative = no color change
Nitrate reduction test reagents - CORRECT ANSWER - -Sulanilic acid
-Dimethyl-alpha-napthylamine
If the Nitrate reduction test is negative, add zinc to determine if the MO can convert nitrate --
>nitrite.
-No color change = positive
-Red = negative - CORRECT ANSWER - What do you do if the initial nitrate reductase
reaction is negative?
Pseudomonas aeruginosa (PSAR) - CORRECT ANSWER - -Large, spready colony
-Iridescent sheen
-Beta hemolysis
***Smells like grapes or corn chips***
Pseudomonas aeruginosa ID tests - CORRECT ANSWER - -NLF
-Oxidase +
-Glucose oxidizer(+air, = closed)
-Growth @ 42 degrees
-Nitrate +
-Pyocyanin
, Acinetobacter baumanii - CORRECT ANSWER - -GN coccobacilli
-Most common isolate from human specimens
-Most common for HAI**
Acinetobacter baumanii ID tests - CORRECT ANSWER - -Slight pink on MAC
-Oxidase =
-Nitrate =
-OF glucose-saccharolytic (A. baumanii)
-OF glucose-asaccharolytic(A. lowfii)
Stenotrophomonas maltophilia - CORRECT ANSWER - -Aquatic environment
-Oxidae =
-Uses glucose and maltose
-Susceptible to SXT disk
Burkholderia cepacia - CORRECT ANSWER - -Nosocomial infection
-Cepacia syndrome- severe respiratory disease in cystic fibrosis patients
Burkholderia cepacia media - CORRECT ANSWER - BCSA and OFPBL, grows yellow
colonies
Burkholderia pseudomallei - CORRECT ANSWER - -Vietnamese time bomb
-Melioidosis
-Bi-polar staining
Flavobacteria - CORRECT ANSWER - -Oxidase +
-yellow/green pigmented colonies
ACTUAL Exam Questions and CORRECT
Answers
Nonfermentors vs enterics - CORRECT ANSWER - -Oxidase positive
-OF glucose test
-Colorless growth on MAC
Nonfermentors - CORRECT ANSWER - MO's that cant metabolize sugars in anaerobic
conditions
Fermentor - CORRECT ANSWER - Utilize carbohydrates for energy both aerobically and
anaerobically
Oxidizer - CORRECT ANSWER - Metabolizes carbohydrates in ari
Non-oxidizer - CORRECT ANSWER - Cannot utilize sugar as an energy source
Oxidative-Fermentative Test - CORRECT ANSWER - -Determines if a MO is a
fermentor, oxidizer or non-oxidizer
***Make sure to test each MO aerobically and anaerobically***
Oxidative-Fermentative Test (Nonfermentor, non oxidizer) - CORRECT ANSWER -
Describe the reaction for tube set one
Oxidative-Fermentative Test (Nonfermentor, oxidizer) - CORRECT ANSWER - Describe
the reaction for tube set two
,Oxidative-Fermentative Test (Fermentor, oxidizer) - CORRECT ANSWER - Describe the
reaction for tube set three
Nitrate reduction test - CORRECT ANSWER - -Determine in MO contains nitrate
reductase (convert nitrate -->nitrite)
-Positive = red
-Negative = no color change
Nitrate reduction test reagents - CORRECT ANSWER - -Sulanilic acid
-Dimethyl-alpha-napthylamine
If the Nitrate reduction test is negative, add zinc to determine if the MO can convert nitrate --
>nitrite.
-No color change = positive
-Red = negative - CORRECT ANSWER - What do you do if the initial nitrate reductase
reaction is negative?
Pseudomonas aeruginosa (PSAR) - CORRECT ANSWER - -Large, spready colony
-Iridescent sheen
-Beta hemolysis
***Smells like grapes or corn chips***
Pseudomonas aeruginosa ID tests - CORRECT ANSWER - -NLF
-Oxidase +
-Glucose oxidizer(+air, = closed)
-Growth @ 42 degrees
-Nitrate +
-Pyocyanin
, Acinetobacter baumanii - CORRECT ANSWER - -GN coccobacilli
-Most common isolate from human specimens
-Most common for HAI**
Acinetobacter baumanii ID tests - CORRECT ANSWER - -Slight pink on MAC
-Oxidase =
-Nitrate =
-OF glucose-saccharolytic (A. baumanii)
-OF glucose-asaccharolytic(A. lowfii)
Stenotrophomonas maltophilia - CORRECT ANSWER - -Aquatic environment
-Oxidae =
-Uses glucose and maltose
-Susceptible to SXT disk
Burkholderia cepacia - CORRECT ANSWER - -Nosocomial infection
-Cepacia syndrome- severe respiratory disease in cystic fibrosis patients
Burkholderia cepacia media - CORRECT ANSWER - BCSA and OFPBL, grows yellow
colonies
Burkholderia pseudomallei - CORRECT ANSWER - -Vietnamese time bomb
-Melioidosis
-Bi-polar staining
Flavobacteria - CORRECT ANSWER - -Oxidase +
-yellow/green pigmented colonies
