ACTUAL Exam Questions and CORRECT
Answers
Of the following choices, which best describe the microscopic appearance of a well made, well
stained peripheral smear? Select all that apply.
A. RBC should appear orange to salmon pink
B. WBC nuclei should be purple to blue
C. Cytoplasm of neutrophils should be pink to tan with violet or lilac granules
D. Eosinophils should have bright orange refractile granules - CORRECT ANSWER - A,
B, C, & D
Of the following choices, which describe the macroscopic appearance of a well made, well
stained smear? Choose all that apply.
A. Pink to purple in color
B. Contain a smooth feathered edge
C. The smear should cover two thirds of the area of the slide
D. Visible ridges, lines and holes in the smear. - CORRECT ANSWER - A, B, & C
What is the appropriate corrective action if RBCs appear gray and WBCs are too dark
(excessively blue) in peripheral smear? - CORRECT ANSWER - Decrease staining time
, What is the appropriate corrective action if Red blood cells too pale or appear red and WBCs are
barely visible? - CORRECT ANSWER - Increase staining time, decrease rinsing time
What is the appropriate corrective action if appearance of water artifacts on the smear
(appearance of moth eaten look to RBCs, refractive blotches on the RBC, crenation appearance
of RBCs)? - CORRECT ANSWER - Dry slides as quickly as possible or fix slides with
methanol
What is the final magnification when using oil power?
A. 100X
B. 1,000X
C. 10,000X
D. 10X - CORRECT ANSWER - B. 1,000X
What is the correct purpose during a peripheral blood film examination in objective 10X? -
CORRECT ANSWER - Select good area. WBC Distribution and RBC distribution. Quick
scan for any abnormal cells such as Blasts, reactive lymphocytes or parasites.
What is the correct purpose during a peripheral blood film examination in objective 40X? -
CORRECT ANSWER - WBC estimate, RBCs should be evenly distributed (2-3 may
overlap).
What is the correct purpose during a peripheral blood film examination in objective 100X? -
CORRECT ANSWER - Identify WBCs for differential, observe platelets (estimate
number, size and morphology)