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UCF Biology Exit Exam Practice Test — 50 Questions
Name: ____________________
Date: _____________________
Instructions: Choose the best answer (A, B, C, or D). Each question
is followed by the correct answer and explanation for your review.
1. What is the smallest unit of life?
A) Atom
B) Molecule
C) Cell
D) Organ
Answer: C — Cell
Explanation: Cells are the fundamental units that carry out all life
processes.
, 2. Which organelle is known as the powerhouse of the cell?
A) Chloroplast
B) Ribosome
C) Mitochondrion
D) Lysosome
Answer: C — Mitochondrion
Explanation: Mitochondria produce ATP through cellular respiration,
supplying energy.
3. What macromolecule primarily makes up enzymes?
A) Lipids
B) Carbohydrates
C) Proteins
D) Nucleic acids
Answer: C — Proteins
Explanation: Enzymes are proteins that catalyze biochemical reactions.
4. What process moves molecules from high to low concentration
without energy?
A) Active transport
B) Facilitated diffusion
C) Diffusion
D) Endocytosis
Answer: C — Diffusion
Explanation: Diffusion is passive movement of molecules down their
concentration gradient.
, 5. Which phase of mitosis involves chromosome separation?
A) Prophase
B) Metaphase
C) Anaphase
D) Telophase
Answer: C — Anaphase
Explanation: Sister chromatids are pulled apart toward opposite poles
during anaphase.
6. DNA replication is considered semi-conservative because:
A) Both new strands are newly synthesized
B) Each new DNA molecule contains one original strand and one
new strand
C) DNA is replicated without enzymes
D) It only occurs in prokaryotes
Answer: B — Each new DNA molecule contains one original and
one new strand
Explanation: The original strand serves as a template for synthesis of a
complementary strand.
7. Which process converts glucose into pyruvate and generates
ATP anaerobically?
A) Krebs cycle
B) Glycolysis
C) Electron transport chain
D) Fermentation
Answer: B — Glycolysis
Explanation: Glycolysis breaks glucose into pyruvate, producing ATP
without oxygen.
, 8. What is the role of chlorophyll in photosynthesis?
A) Absorb water
B) Capture light energy
C) Fix carbon dioxide
D) Store glucose
Answer: B — Capture light energy
Explanation: Chlorophyll absorbs sunlight to energize electrons in
photosynthesis.
9. Which kingdom contains multicellular organisms that perform
photosynthesis?
A) Fungi
B) Plantae
C) Protista
D) Animalia
Answer: B — Plantae
Explanation: Plants are multicellular autotrophs that use photosynthesis.
10. Which organelle is responsible for modifying and
packaging proteins?
A) Golgi apparatus
B) Endoplasmic reticulum
C) Ribosome
D) Lysosome
Answer: A — Golgi apparatus
Explanation: Golgi processes and packages proteins for transport.