MOCK CG(ASCP) CERTIFICATION EXAM 1 -
PART 1 QUESTIONS AND ANSWERS LATEST
2025-2026 UPDATE
Which of the following is NOT an advantage that in situ coverslip
amniotic cultures have over flask amniotic fluid cultures?
a. Decreased the turnaround time.
b. Increases the likelihood of distinguishing between true mosaicism and
psuedomosaicisme.
c. Can be used to rule out maternal contamination when the fetus is
female.
d. Can determine if an abnormal clone is arising from one or more
colonies. - Answer-Can be used to rule out maternal contamination when
the fetus is female.
Collagenase is used in culture of solid tissues to?
a. Dissociate cells
b. To supplement culture media
c. To prevent mycoplasma contamination
c. To subculture the monolayer cultures - Answer-Dissociate cells
What microscope is useful in the separation of fetal tissue from
maternal tissue?
a. Brightfield microscope
b. Fluorescent microscope
,c. Compound microscope
d. Stereo (dissecting) - microscope - Answer-Stereo (dissecting) -
microscope
Which of the following should be added to modified media before use
in amniotic fluid cultures?
a. Fetal Bovine Serum (FBS)
b. Vitamins
c. Sucrose
d. Gentamycin - Answer-Fetal Bovine Serum (FBS)
Which the following is often added to media, salt solutions, and
enzymes as a pH indicator?
a. Sodium bicarbonate
b. CO2
c. Hepes buffer
d. Phenol red - Answer-Phenol red
Which of the following peripheral blood cultures stimulated with PHA
can be used for chromosome preparations?
a. 48 hr
b. 72 hr
c. 92 hr
d. All of the above - Answer-All of the above
, Which of the following biological safety cabinets will allow microbial
contamination of the specimen if used during tissue culture?
a. Horizontal laminar flow hood
b. Biological safety cabinets Class 1
c. Biological safety cabinets Class 2
d. Biological safety cabinets Class 3 - Answer-Biological safety cabinets
Class 1
To prevent cross contamination of cultures, a tissue culturist SHOULD?
a. Use the same media bottle for long term and primary cultures.
b. Work with a transformed culture at the same time in the culture hood
as a primary culture.
c. Change the pipette after feeding cultures from different patients.
d. Touch the lip of the media bottle against the top of the flask being fed.
- Answer-Change the pipette after feeding cultures from different
patients.
When subculturing a long-term cell line, a technician finds that the pH
of the media has turned a yellow color overnight. When looked at
through the inverted microscope many small dot structures can be seen
around the cells. Which of the following may be true about this culture?
a. The medium has turned yellow because these cells are dividing very
rapidly and need to be sub-cultured.
b. The acidic media and dot-like structures are the result of cell death
due to overgrowth of the cell culture.
, c. This culture is contaminated with a microbe which is making the
media acidic.
d. This is a normal phenomenon observed in cell culturing. - Answer-
This culture is contaminated with a microbe which is making the media
acidic.
The inverted microscope is essential in the monitoring of cells cultures
by allowing the technologist to visualize all but which of the following
cellular features?
a. Cellular confluency
b. Chromosomal abnormalities
c. Mitotic cells
d. Cellular attachment - Answer-Chromosomal abnormalities
Amniotic fluid cultures grown in the flask method are considered to be
ready for harvesting when they are?
a. 50% confluent
b. 75% confluent
c. 90% confluent
d. 100% confluent - Answer-75% confluent
In order to store cells for later study experiments, scientists will
cryopreserve cells at extremely low temperature (-90 'C to 195 'C). What
is the function of the freezing solution?
a. This solution dehydrates the cell
PART 1 QUESTIONS AND ANSWERS LATEST
2025-2026 UPDATE
Which of the following is NOT an advantage that in situ coverslip
amniotic cultures have over flask amniotic fluid cultures?
a. Decreased the turnaround time.
b. Increases the likelihood of distinguishing between true mosaicism and
psuedomosaicisme.
c. Can be used to rule out maternal contamination when the fetus is
female.
d. Can determine if an abnormal clone is arising from one or more
colonies. - Answer-Can be used to rule out maternal contamination when
the fetus is female.
Collagenase is used in culture of solid tissues to?
a. Dissociate cells
b. To supplement culture media
c. To prevent mycoplasma contamination
c. To subculture the monolayer cultures - Answer-Dissociate cells
What microscope is useful in the separation of fetal tissue from
maternal tissue?
a. Brightfield microscope
b. Fluorescent microscope
,c. Compound microscope
d. Stereo (dissecting) - microscope - Answer-Stereo (dissecting) -
microscope
Which of the following should be added to modified media before use
in amniotic fluid cultures?
a. Fetal Bovine Serum (FBS)
b. Vitamins
c. Sucrose
d. Gentamycin - Answer-Fetal Bovine Serum (FBS)
Which the following is often added to media, salt solutions, and
enzymes as a pH indicator?
a. Sodium bicarbonate
b. CO2
c. Hepes buffer
d. Phenol red - Answer-Phenol red
Which of the following peripheral blood cultures stimulated with PHA
can be used for chromosome preparations?
a. 48 hr
b. 72 hr
c. 92 hr
d. All of the above - Answer-All of the above
, Which of the following biological safety cabinets will allow microbial
contamination of the specimen if used during tissue culture?
a. Horizontal laminar flow hood
b. Biological safety cabinets Class 1
c. Biological safety cabinets Class 2
d. Biological safety cabinets Class 3 - Answer-Biological safety cabinets
Class 1
To prevent cross contamination of cultures, a tissue culturist SHOULD?
a. Use the same media bottle for long term and primary cultures.
b. Work with a transformed culture at the same time in the culture hood
as a primary culture.
c. Change the pipette after feeding cultures from different patients.
d. Touch the lip of the media bottle against the top of the flask being fed.
- Answer-Change the pipette after feeding cultures from different
patients.
When subculturing a long-term cell line, a technician finds that the pH
of the media has turned a yellow color overnight. When looked at
through the inverted microscope many small dot structures can be seen
around the cells. Which of the following may be true about this culture?
a. The medium has turned yellow because these cells are dividing very
rapidly and need to be sub-cultured.
b. The acidic media and dot-like structures are the result of cell death
due to overgrowth of the cell culture.
, c. This culture is contaminated with a microbe which is making the
media acidic.
d. This is a normal phenomenon observed in cell culturing. - Answer-
This culture is contaminated with a microbe which is making the media
acidic.
The inverted microscope is essential in the monitoring of cells cultures
by allowing the technologist to visualize all but which of the following
cellular features?
a. Cellular confluency
b. Chromosomal abnormalities
c. Mitotic cells
d. Cellular attachment - Answer-Chromosomal abnormalities
Amniotic fluid cultures grown in the flask method are considered to be
ready for harvesting when they are?
a. 50% confluent
b. 75% confluent
c. 90% confluent
d. 100% confluent - Answer-75% confluent
In order to store cells for later study experiments, scientists will
cryopreserve cells at extremely low temperature (-90 'C to 195 'C). What
is the function of the freezing solution?
a. This solution dehydrates the cell
