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How would you recognize colonies of E. coli coliforms, non E.coli coliforms, and non coliforms
on Petrifilm agar? E. coli coliforms: blue w/ gas
non E.coli coliforms: red w/ gas
non coliforms: red w/ no gas
Endogenous infections in the urinary tract are caused most commonly by what three bacteria?
Why are women more prone to urinary tract infections compared to men? E. Coli, Proteus,
Klebsiella.
Urethra is closer to anus.
Condoms cannot provide protection against which two STDs? HPV and herpes
What are advantages and disadvantages of live attenuated pathogen? advantage: only
needs 1 shot
disadvantage: can revert back
What are advantages and disadvantages of purified antigen from pathogen and genetically
engineered vaccine?? advantage: takes away side effects
disadvantage: only stimulates humoral response
What are advantages and disadvantages of purified antigen from pathogen and genetically
engineered vaccine?? advantage: takes away side effects
disadvantage: only stimulates humoral response
,What are advantages and disadvantages of toxoid vaccine? advantage: less risk of side
effects
disadvantages: only stimulates humoral response
Explain why the preparation of yogurt is a microbial succession? One organism is required to
create the acidic environment required for the other
How do defensins kill bacteria? Destroy the bacterial cell membrane
What is an antibiotic? chemical produced by a living organism that stops the growth of a
bacteria
What is selective toxicity? kill microbial cells but not the host's cells
What are Koch's postulates? 1. take diseased animal's cells and make a streak plate
2. isolate a pure culture
3. infect healthy animal
4. re isolate agent from newly infected animal and compare
Infant botulism is an _____ and adult botulism is an _____. infection
intoxication
T/F: toxins are ALWAYS proteins except endotoxins True
Why does HIV suppress the immune system? it decreases the number of T helper cells due
to the CD4 receptor. Without T helpers, our immune response won't work
, What does protein A do? blocks the Fc stem of Ab from binding to PMN
T/F: cranberry juice is used to cure a UTI false, can only prevent it
T/F: when nitrate is converted to nitrate, E. coli is present True
T/F: A positive nitrate test will be a pink dipstick True
T/F: Gardasil is a recombinant DNA vaccine True
What synthesis is inhibited and the MOA for penicillin, methicillin, carbenicillin, carbapenum,
cephalosporin, celolopthin, and amoxicillin? inhibits peptidoglycan synthesis
binds to transpeptidase, preventing cross linkages
What synthesis is inhibited and the MOA for bacitracin? it inhibits peptidoglycan,
prevents cross linkages of Nam and Nag
What is the structure of chromosomes on procaryotes vs eucaryotes? procaryotes: CCC
(covalently circular chromosome)
eucaryotes: linear
What synthesis is inhibited and the MOA for vancomycin? inhibits peptidoglycan,
hides DALA DALA , so transpeptidase cant bind
What synthesis is inhibited and the MOA for rifampicin? inhibits DNA/RNA,
binds to RNAP and prevents transcription