MLPAO Test Prep ~ Hematology EXAM ,
With Complete solution 2025/2026
What are the three methods of Peripheral blood smears (PBS)? - ANSWERS-Wedge
method (manual), Spun method (automated slide spinner), and automated method
(analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader
slide ___ - ANSWERS-Decreased
Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. -
ANSWERS-First
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader
slide ___ - ANSWERS-Increased
HCT Values: Male, Female - ANSWERS-0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide - ANSWERS-2/3, ¾
Good blood film should have a slightly ___ or ____ edge - ANSWERS-Rounded,
feathered
The angle of the spreader slide is approximately ___ degrees - ANSWERS-35
The greater the angle of the spreader slide the ____ and _____ the film - ANSWERS-
Shorter, Thicker
The lower the angle of the spreader slide the ___ and ____ the film - ANSWERS-
Longer and Thinner
Blood film should be allowed to ____ ___ completely before staining procedures -
ANSWERS-air dry
Fixative found in stain is - ANSWERS-Methanol
Slides are washed with buffer of pH ___ to develop colour on the slide - ANSWERS-6.4
Stain is comprised of ____ and ____ dyes - ANSWERS-Basic, acidic
What is the basic (cationic) dye? - ANSWERS-Methylene blue
, Methylene blue stains the ___ of the cell and the granules of _____ (____ in colour) -
ANSWERS-Nucleus, basophils, blue-grey
What is the acidic (anionic) dye? - ANSWERS-Eosin
Eosin stains ___ and the granules of ____ (_____ in colour) - ANSWERS-Hemoglobin,
eosinophils, red-orange
What does CBC stand for? - ANSWERS-Complete Blood Count
What are the formed elements of blood? - ANSWERS-WBC, RBC, and Platelets
EDTA Anticoagulant - ANSWERS-Maintains cell shape and size and prevents blood
from clotting by binding to calcium
Heparin Anticoagulant - ANSWERS-Initiates cell clumping and provides blue
background staining
Citrate Anticoagulant - ANSWERS-Dilutes the blood due to the volume of the liquid and
is used for platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing - ANSWERS-4
CBC handling and storage: When transporting to other labs/hospitals - ANSWERS-
surround with ice packs
CBC handling and storage: Samples are _____ before testing - ANSWERS-mixed well
by inversion
CBC handling and storage: Samples must be tested within __ - ANSWERS-24h
CBC handling and storage: Peripheral blood smears must be made within - ANSWERS-
24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED
FOR TESTING - ANSWERS-24h
What happens if CBC is tested after 24h? - ANSWERS-Platelets clump, WBC nuclei
disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs swell and
break down
Coagulation: Specimen of choice? - ANSWERS-Plasma
Coagulation: What colour tube? What is the additive? - ANSWERS-light blue, sodium
citrate
With Complete solution 2025/2026
What are the three methods of Peripheral blood smears (PBS)? - ANSWERS-Wedge
method (manual), Spun method (automated slide spinner), and automated method
(analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader
slide ___ - ANSWERS-Decreased
Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. -
ANSWERS-First
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader
slide ___ - ANSWERS-Increased
HCT Values: Male, Female - ANSWERS-0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide - ANSWERS-2/3, ¾
Good blood film should have a slightly ___ or ____ edge - ANSWERS-Rounded,
feathered
The angle of the spreader slide is approximately ___ degrees - ANSWERS-35
The greater the angle of the spreader slide the ____ and _____ the film - ANSWERS-
Shorter, Thicker
The lower the angle of the spreader slide the ___ and ____ the film - ANSWERS-
Longer and Thinner
Blood film should be allowed to ____ ___ completely before staining procedures -
ANSWERS-air dry
Fixative found in stain is - ANSWERS-Methanol
Slides are washed with buffer of pH ___ to develop colour on the slide - ANSWERS-6.4
Stain is comprised of ____ and ____ dyes - ANSWERS-Basic, acidic
What is the basic (cationic) dye? - ANSWERS-Methylene blue
, Methylene blue stains the ___ of the cell and the granules of _____ (____ in colour) -
ANSWERS-Nucleus, basophils, blue-grey
What is the acidic (anionic) dye? - ANSWERS-Eosin
Eosin stains ___ and the granules of ____ (_____ in colour) - ANSWERS-Hemoglobin,
eosinophils, red-orange
What does CBC stand for? - ANSWERS-Complete Blood Count
What are the formed elements of blood? - ANSWERS-WBC, RBC, and Platelets
EDTA Anticoagulant - ANSWERS-Maintains cell shape and size and prevents blood
from clotting by binding to calcium
Heparin Anticoagulant - ANSWERS-Initiates cell clumping and provides blue
background staining
Citrate Anticoagulant - ANSWERS-Dilutes the blood due to the volume of the liquid and
is used for platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing - ANSWERS-4
CBC handling and storage: When transporting to other labs/hospitals - ANSWERS-
surround with ice packs
CBC handling and storage: Samples are _____ before testing - ANSWERS-mixed well
by inversion
CBC handling and storage: Samples must be tested within __ - ANSWERS-24h
CBC handling and storage: Peripheral blood smears must be made within - ANSWERS-
24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED
FOR TESTING - ANSWERS-24h
What happens if CBC is tested after 24h? - ANSWERS-Platelets clump, WBC nuclei
disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs swell and
break down
Coagulation: Specimen of choice? - ANSWERS-Plasma
Coagulation: What colour tube? What is the additive? - ANSWERS-light blue, sodium
citrate
