BIOD 171 MODULE 3 SAMPLE EXAM QUESTIONS
WITH ANSWERS
light microscope - ✅✅use visible light and blue wavelengths for improved
resolution, magnification 1x-2000x resolution: 10nm-200nm can see whole cells
and organelles
electron microscope - ✅✅uses electrons with specimens in a vacuum, mag
1000x-100,000x resolution: 1mm - 0.1 nm prokaryotic cells, viruses,
macromolecules and large atoms
probe microscope - ✅✅electron probes that move over the specimen service,
mag: greater than 100,000,000x resolution: 0.01nm - 10 nm, 3D surface of
macromolecules and atoms
Bright-field - ✅✅count microorganisms or cells, view stained specimen, view
live cells, staining usually required, bright background with clear or colored
specimen
phase contrast microscope - ✅✅view internal structures of live specimens,
observe motility, no staining necessary so we can view live specimens, image
shows light and dark areas of microbe
, Dark field - ✅✅view living and unstained specimens, filter inhibits light from
going through the organism and instead light is reflected by organism, dark
background with bright specimen.
Fluorescnce Microscopy - ✅✅localize specific structures or molecules,
diagnostic tool, uses UV light to excite fluorophores, visualize whole cells, specific
structures, or proteins and watch movement or interactions, dark backgrounds with
fluorescent structures
Immunifluorescence - ✅✅fluorescent dyes are linked to antibodies which find a
cellular target and bind to it bringing the dye that can visualize and locate the
structures
confocal microscopy - ✅✅highly detailed structures, 3D renderings, biofilms,
uses a laser to focus plane by plane through the specimen, single plain of structures
stained with fluorescent dyes
why do we stain microbes - ✅✅improves resolution and contrast of a sample for
visualization via light microscopy
how does a brightfield microscope work - ✅✅before the light reaches the sample
it passes through a condenser converging the light beams into a focused area on
WITH ANSWERS
light microscope - ✅✅use visible light and blue wavelengths for improved
resolution, magnification 1x-2000x resolution: 10nm-200nm can see whole cells
and organelles
electron microscope - ✅✅uses electrons with specimens in a vacuum, mag
1000x-100,000x resolution: 1mm - 0.1 nm prokaryotic cells, viruses,
macromolecules and large atoms
probe microscope - ✅✅electron probes that move over the specimen service,
mag: greater than 100,000,000x resolution: 0.01nm - 10 nm, 3D surface of
macromolecules and atoms
Bright-field - ✅✅count microorganisms or cells, view stained specimen, view
live cells, staining usually required, bright background with clear or colored
specimen
phase contrast microscope - ✅✅view internal structures of live specimens,
observe motility, no staining necessary so we can view live specimens, image
shows light and dark areas of microbe
, Dark field - ✅✅view living and unstained specimens, filter inhibits light from
going through the organism and instead light is reflected by organism, dark
background with bright specimen.
Fluorescnce Microscopy - ✅✅localize specific structures or molecules,
diagnostic tool, uses UV light to excite fluorophores, visualize whole cells, specific
structures, or proteins and watch movement or interactions, dark backgrounds with
fluorescent structures
Immunifluorescence - ✅✅fluorescent dyes are linked to antibodies which find a
cellular target and bind to it bringing the dye that can visualize and locate the
structures
confocal microscopy - ✅✅highly detailed structures, 3D renderings, biofilms,
uses a laser to focus plane by plane through the specimen, single plain of structures
stained with fluorescent dyes
why do we stain microbes - ✅✅improves resolution and contrast of a sample for
visualization via light microscopy
how does a brightfield microscope work - ✅✅before the light reaches the sample
it passes through a condenser converging the light beams into a focused area on
