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BIOD 171 MODULE 3 EXAM QUESTIONS WITH CORRECT ANSWERS GRADED A+

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What type of cell does the Giemsa stain? - White blood cells What type of samples are acid-fast stains often performed on? - Sputum samples (thick mucus produced in lungs that can be coughed up) The naked eye can see up to ______ micrometers. - 100 micrometers Cellular organelles can be as small as ______ micrometers. - 0.2 micrometers Does phase contrast need staining? - No What is the difference between phase contrast compared to brightfield? - You are able to visualize things that would otherwise be invisible; cells are highly distinguishable What type of microscopy uses lasers? - Confocal Does electron microscopy use fixation? - Yes

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BIOD 171 MODULE 3 EXAM QUESTIONS WITH
CORRECT ANSWERS GRADED A+


What type of cell does the Giemsa stain? - ✅✅White blood cells



What type of samples are acid-fast stains often performed on? - ✅✅Sputum
samples (thick mucus produced in lungs that can be coughed up)



The naked eye can see up to ______ micrometers. - ✅✅100 micrometers



Cellular organelles can be as small as ______ micrometers. - ✅✅0.2
micrometers



Does phase contrast need staining? - ✅✅No



What is the difference between phase contrast compared to brightfield? -
✅✅You are able to visualize things that would otherwise be invisible; cells are
highly distinguishable



What type of microscopy uses lasers? - ✅✅Confocal

, Does electron microscopy use fixation? - ✅✅Yes



Does electron microscopy use live or dead samples? - ✅✅Dead



What type of secondary (counterstain) dye called in gram staining? -
✅✅Safranin



___________________ ______________ microscopy coats samples with gold of
platinum. - ✅✅Scanning electron microscopy



Does negative staining kill the organism and why? - ✅✅No. Since negative
staining is mildly invasive, it may not kill the microorganism.



Who is the father of medicine? - ✅✅Hippocrates



What is microscopy? - ✅✅Use of light or electrons to magnify objects



What are the two measurements of microscopy? - ✅✅Micrometer and
nanometer



Magnification - ✅✅Increase the size of object

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