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MB (ASCP) Exam Questions And Answers
|Latest 2025 | Guaranteed Pass
Predominant form of DNA - Answer✔Right-handed B
DNA and histone interaction is due to what type of bond? - Answer✔Ionic
What stains to the minor groove of DNA duplex? - Answer✔SYBR Green
What is the most abundant type of RNA? - Answer✔Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - Answer✔Genome and mRNA
What increases half-life of mRNA? - Answer✔5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - Answer✔Euchromatin
What is the most common type of eukaryotic DNA? - Answer✔Satellite DNA
What specimen has the most DNA according to molecular weight? - Answer✔White Blood Cells
What amino acids compose histones? - Answer✔Lysine and Arginine
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, ©BRIGHTSTARS 2025 ALL RIGHTS RESERVED 9:46 AM A+
Amino acid that all proteins start with: - Answer✔Methionine
What protects the DNA strand during replication? - Answer✔Single-stranded binding protein
Leading Strand - Answer✔3' to 5' template
Lagging Strand - Answer✔5' to 3' template
What DNA strand is being copied in transcription? - Answer✔Antisense Strand
First step after newly formed mRNA is made? - Answer✔Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
Answer✔Telomerase
What polymerase has repair activity? - Answer✔Beta/Epsilon
What polymerase acts on leading strand? - Answer✔Delta
What polymerase acts on lagging strand? - Answer✔Alpha
Which polymerase is used for mitochondrial replication and repair? - Answer✔Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔Topoisomerase
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, ©BRIGHTSTARS 2025 ALL RIGHTS RESERVED 9:46 AM A+
Which RNA polymerase is mainly for transcription? - Answer✔RNA Pol II
Which polymerase is involved with mRNA synthesis? - Answer✔RNA Pol II
What inactivates RNAses? - Answer✔Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6
hours at 400C
What type of restriction enzyme recognizes palindromic sites? - Answer✔Restriction Enzyme
Type II
What type of restriction enzyme lacks methylation activity? - Answer✔Restriction Enzyme Type
II
What is necessary to make cDNA? - Answer✔Reverse transcriptase
Number of SNPs in a human genome? - Answer✔11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - Answer✔3 billion base pairs
The polymerization of acrylamide requires: - Answer✔Catalyst (APS or TEMED) or Light
activation
How does agarose gel pore size relate to concentration? - Answer✔Inversely proportional
What is denatured during a southern blot? - Answer✔Hydrogen bonds (inter-strand bonds)
Melting temperature formula - Answer✔Tm = (4C x GC Pairs) + (2C x AT pairs)
3
MB (ASCP) Exam Questions And Answers
|Latest 2025 | Guaranteed Pass
Predominant form of DNA - Answer✔Right-handed B
DNA and histone interaction is due to what type of bond? - Answer✔Ionic
What stains to the minor groove of DNA duplex? - Answer✔SYBR Green
What is the most abundant type of RNA? - Answer✔Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - Answer✔Genome and mRNA
What increases half-life of mRNA? - Answer✔5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - Answer✔Euchromatin
What is the most common type of eukaryotic DNA? - Answer✔Satellite DNA
What specimen has the most DNA according to molecular weight? - Answer✔White Blood Cells
What amino acids compose histones? - Answer✔Lysine and Arginine
1
, ©BRIGHTSTARS 2025 ALL RIGHTS RESERVED 9:46 AM A+
Amino acid that all proteins start with: - Answer✔Methionine
What protects the DNA strand during replication? - Answer✔Single-stranded binding protein
Leading Strand - Answer✔3' to 5' template
Lagging Strand - Answer✔5' to 3' template
What DNA strand is being copied in transcription? - Answer✔Antisense Strand
First step after newly formed mRNA is made? - Answer✔Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
Answer✔Telomerase
What polymerase has repair activity? - Answer✔Beta/Epsilon
What polymerase acts on leading strand? - Answer✔Delta
What polymerase acts on lagging strand? - Answer✔Alpha
Which polymerase is used for mitochondrial replication and repair? - Answer✔Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔Topoisomerase
2
, ©BRIGHTSTARS 2025 ALL RIGHTS RESERVED 9:46 AM A+
Which RNA polymerase is mainly for transcription? - Answer✔RNA Pol II
Which polymerase is involved with mRNA synthesis? - Answer✔RNA Pol II
What inactivates RNAses? - Answer✔Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6
hours at 400C
What type of restriction enzyme recognizes palindromic sites? - Answer✔Restriction Enzyme
Type II
What type of restriction enzyme lacks methylation activity? - Answer✔Restriction Enzyme Type
II
What is necessary to make cDNA? - Answer✔Reverse transcriptase
Number of SNPs in a human genome? - Answer✔11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - Answer✔3 billion base pairs
The polymerization of acrylamide requires: - Answer✔Catalyst (APS or TEMED) or Light
activation
How does agarose gel pore size relate to concentration? - Answer✔Inversely proportional
What is denatured during a southern blot? - Answer✔Hydrogen bonds (inter-strand bonds)
Melting temperature formula - Answer✔Tm = (4C x GC Pairs) + (2C x AT pairs)
3
