MB (ASCP) EXAM WITH CORRECT ACTUAL AND
CORRECTLY WELL-DEFINED ANSWERS LATEST
2025-2026 GRADED A+
What is necessary to make cDNA? Correct Answer Reverse
transcriptase
Number of SNPs in a human genome? Correct Answer 11 billion
(10^7)
How many genes (base pairs) are in a human haploid genome?
Correct Answer 3 billion base pairs
The polymerization of acrylamide requires: Correct Answer
Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? Correct
Answer Inversely proportional
What is denatured during a southern blot? Correct Answer
Hydrogen bonds (inter-strand bonds)
,Melting temperature formula Correct Answer Tm = (4C x GC
Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? Correct Answer
5' G
Proper GC content of primer Correct Answer 35-65%
Inhibitors of PCR Correct Answer Hemoglobin, Heparin, Xylene,
Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction: Correct Answer Master mix >
patient sample > positive control > negative control
Pre Area Correct Answer Positive Pressure
Post Area Correct Answer Negative Pressure
Which PCR requires two steps for specificity? Correct Answer
Nested PCR
Which microorganism is detected by branched DNA amplification?
Correct Answer DNA or RNA
, Which technique uses RNA, antibodies, and DNA? Correct
Answer Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of
methylation Correct Answer Bisulfite DNA
What tests are isothermal? Correct Answer NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? Correct Answer
AML
T-cell gene rearrangement is seen in what malignancy? Correct
Answer Sezary Syndrome
Which translocation causes Acute Premyelocytic Leukemia?
Correct Answer t 15;17
Ewing Sarcoma Correct Answer t 11;22
Synovial Sarcoma Correct Answer t X;18
Follicular Lymphoma Correct Answer t 14;18
CORRECTLY WELL-DEFINED ANSWERS LATEST
2025-2026 GRADED A+
What is necessary to make cDNA? Correct Answer Reverse
transcriptase
Number of SNPs in a human genome? Correct Answer 11 billion
(10^7)
How many genes (base pairs) are in a human haploid genome?
Correct Answer 3 billion base pairs
The polymerization of acrylamide requires: Correct Answer
Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? Correct
Answer Inversely proportional
What is denatured during a southern blot? Correct Answer
Hydrogen bonds (inter-strand bonds)
,Melting temperature formula Correct Answer Tm = (4C x GC
Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? Correct Answer
5' G
Proper GC content of primer Correct Answer 35-65%
Inhibitors of PCR Correct Answer Hemoglobin, Heparin, Xylene,
Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction: Correct Answer Master mix >
patient sample > positive control > negative control
Pre Area Correct Answer Positive Pressure
Post Area Correct Answer Negative Pressure
Which PCR requires two steps for specificity? Correct Answer
Nested PCR
Which microorganism is detected by branched DNA amplification?
Correct Answer DNA or RNA
, Which technique uses RNA, antibodies, and DNA? Correct
Answer Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of
methylation Correct Answer Bisulfite DNA
What tests are isothermal? Correct Answer NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? Correct Answer
AML
T-cell gene rearrangement is seen in what malignancy? Correct
Answer Sezary Syndrome
Which translocation causes Acute Premyelocytic Leukemia?
Correct Answer t 15;17
Ewing Sarcoma Correct Answer t 11;22
Synovial Sarcoma Correct Answer t X;18
Follicular Lymphoma Correct Answer t 14;18
