1
MB (ASCP) Exam 2025 update| comprehensive
questions and verified answers (complete solutions)
ASSURED SUCCESS|GRADE A+!! (100% ACCURATE)
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Predominant form of DNA - (answers)Right-handed B
DNA and histone interaction is due to what type of bond? - (answers)Ionic
What stains to the minor groove of DNA duplex? - (answers)SYBR Green
What is the most abundant type of RNA? - (answers)Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - (answers)Genome and mRNA
What increases half-life of mRNA? - (answers)5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -
(answers)Euchromatin
What is the most common type of eukaryotic DNA? - (answers)Satellite DNA
What specimen has the most DNA according to molecular weight? -
(answers)White Blood Cells
, 2
What amino acids compose histones? - (answers)Lysine and Arginine
Amino acid that all proteins start with: - (answers)Methionine
What protects the DNA strand during replication? - (answers)Single-stranded
binding protein
Leading Strand - (answers)3' to 5' template
Lagging Strand - (answers)5' to 3' template
What DNA strand is being copied in transcription? - (answers)Antisense Strand
First step after newly formed mRNA is made? - (answers)Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme? - (answers)Telomerase
What polymerase has repair activity? - (answers)Beta/Epsilon
What polymerase acts on leading strand? - (answers)Delta
, 3
What polymerase acts on lagging strand? - (answers)Alpha
Which polymerase is used for mitochondrial replication and repair? -
(answers)Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - (answers)Topoisomerase
Which RNA polymerase is mainly for transcription? - (answers)RNA Pol II
Which polymerase is involved with mRNA synthesis? - (answers)RNA Pol II
What inactivates RNAses? - (answers)Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -
(answers)Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - (answers)Restriction
Enzyme Type II
What is necessary to make cDNA? - (answers)Reverse transcriptase
MB (ASCP) Exam 2025 update| comprehensive
questions and verified answers (complete solutions)
ASSURED SUCCESS|GRADE A+!! (100% ACCURATE)
GET IT RIGHT!!
Predominant form of DNA - (answers)Right-handed B
DNA and histone interaction is due to what type of bond? - (answers)Ionic
What stains to the minor groove of DNA duplex? - (answers)SYBR Green
What is the most abundant type of RNA? - (answers)Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - (answers)Genome and mRNA
What increases half-life of mRNA? - (answers)5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? -
(answers)Euchromatin
What is the most common type of eukaryotic DNA? - (answers)Satellite DNA
What specimen has the most DNA according to molecular weight? -
(answers)White Blood Cells
, 2
What amino acids compose histones? - (answers)Lysine and Arginine
Amino acid that all proteins start with: - (answers)Methionine
What protects the DNA strand during replication? - (answers)Single-stranded
binding protein
Leading Strand - (answers)3' to 5' template
Lagging Strand - (answers)5' to 3' template
What DNA strand is being copied in transcription? - (answers)Antisense Strand
First step after newly formed mRNA is made? - (answers)Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme? - (answers)Telomerase
What polymerase has repair activity? - (answers)Beta/Epsilon
What polymerase acts on leading strand? - (answers)Delta
, 3
What polymerase acts on lagging strand? - (answers)Alpha
Which polymerase is used for mitochondrial replication and repair? -
(answers)Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - (answers)Topoisomerase
Which RNA polymerase is mainly for transcription? - (answers)RNA Pol II
Which polymerase is involved with mRNA synthesis? - (answers)RNA Pol II
What inactivates RNAses? - (answers)Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? -
(answers)Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - (answers)Restriction
Enzyme Type II
What is necessary to make cDNA? - (answers)Reverse transcriptase