MB(ASCP) PRACTICE EXAM WITH CORRECT
ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST 2025-
2026 ALREADY GRADED A+
How many hydrogen bonds are formed between one C:G base pair?
1
2
3
4 - ANSWERS-3
What is the enzyme responsible for stitching together Okazaki fragments?
Helicase
Single-stranded DNA binding protein
Primase
Ligase - ANSWERS-Ligase
How is warfarin dosage affected in patients with VKORC1 deficiency?
,Patients receive a higher warfarin dose
Patients receive a lower warfarin dose
Warfarin dosage is unaffected - ANSWERS-Patients receive a lower warfarin
dose
When this enzyme finds nicks or gaps in the sugar-phosphate backbone of DNA,
it closes them!
Thermosequenase
Helicase
Primase
Ligase - ANSWERS-Ligase
In which of the following methods is the probe digested by the polymerase
(Taq) during primer extension?
Taqman
FRET probes
Molecular beacons
Scorpion probes - ANSWERS-Taqman
Which of the following is not a source of DNA damage?
Chemical damage
Splicing
Attack by water
,Radiation damage - ANSWERS-Splicing
All of the following are trinucleotide repeat disorders, EXCEPT:
Spinocerebellar Ataxia Type 8
Huntington Disease
Asperger Disease
Fragile X Disease - ANSWERS-Asperger Disease
This polymerase is involved in "short-patch base excision repair":
Pol α
Pol β
Pol γ
Pol δ - ANSWERS-Pol β
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - ANSWERS-1 in 1,000,000,000 base pairs
, The rate of DNA migration through an agarose gel during electrophoresis does
not depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - ANSWERS-Nucleotide sequence of the
molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - ANSWERS-Initiation,
exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - ANSWERS-3'-GAC
ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST 2025-
2026 ALREADY GRADED A+
How many hydrogen bonds are formed between one C:G base pair?
1
2
3
4 - ANSWERS-3
What is the enzyme responsible for stitching together Okazaki fragments?
Helicase
Single-stranded DNA binding protein
Primase
Ligase - ANSWERS-Ligase
How is warfarin dosage affected in patients with VKORC1 deficiency?
,Patients receive a higher warfarin dose
Patients receive a lower warfarin dose
Warfarin dosage is unaffected - ANSWERS-Patients receive a lower warfarin
dose
When this enzyme finds nicks or gaps in the sugar-phosphate backbone of DNA,
it closes them!
Thermosequenase
Helicase
Primase
Ligase - ANSWERS-Ligase
In which of the following methods is the probe digested by the polymerase
(Taq) during primer extension?
Taqman
FRET probes
Molecular beacons
Scorpion probes - ANSWERS-Taqman
Which of the following is not a source of DNA damage?
Chemical damage
Splicing
Attack by water
,Radiation damage - ANSWERS-Splicing
All of the following are trinucleotide repeat disorders, EXCEPT:
Spinocerebellar Ataxia Type 8
Huntington Disease
Asperger Disease
Fragile X Disease - ANSWERS-Asperger Disease
This polymerase is involved in "short-patch base excision repair":
Pol α
Pol β
Pol γ
Pol δ - ANSWERS-Pol β
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - ANSWERS-1 in 1,000,000,000 base pairs
, The rate of DNA migration through an agarose gel during electrophoresis does
not depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - ANSWERS-Nucleotide sequence of the
molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - ANSWERS-Initiation,
exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - ANSWERS-3'-GAC
