Lab #5 ELN Weekly Guidelines - Gibson Assembly, Transformation, and Interim Lab
NOTE - Because we are waiting for colonies to form, your ELN submission will be
postponed.
WED section - ELN due on Friday 6pm
THUR section - ELN due on Monday at 6pm
FRI section - ELN due on Tuesday at 6pm
PURPOSE
❏ Mention all protocols (3) protocol used!
❏ Briefly describe what was done to state WHY it was relevant!
❏ Gibson Assembly, Transformation, and Interim Lab
❏ State how these protocols will help you reach your CaMPARI project goals
*It is a given that you will be learning new lab techniques - this does NOT need to
be stated
METHODS
❏ Copy & Paste all protocols (3)
❏ Gibson Assembly, Transformation, and Interim Lab
❏ Complete the Gibson Assembly Reaction Table.
You do not need to include your plate art, but you are free to! (this does not need
to be analyzed in Results - Data or Text)
RESULTS - DATA
❏ Raw Control and Experimental plate images provided by Lab Manager Staff
❏ Include these as Panel A and Panel B (Multi-panel figure!)
❏ Remember to include appropriate annotations!
❏ Colony Counts Table - make your own or use the example in the slides!
❏ Remember to include appropriate annotations!
RESULTS - TEXT
❏ State all colony counts for each plate and which counting method was followed.
❏ Are these counts expected (do not include why or why not - this belongs in discussion).
❏ I.E.) If the Gibson Assembly went well, are these counts expected to be seen on
the experimental plate and the Control (Digested pHLsec) plate?
❏ Make a brief comparison between your control and experimental plates.
DISCUSSION
❏ What happens during the Gibson Assembly reaction?
❏ Describe the process (brief! 2-3 sentences).
❏ Why was the precise preparation for each Linear piece of DNA so necessary
(brief! 1-2 sentences) to Gibson Assembly prep.
NOTE - Because we are waiting for colonies to form, your ELN submission will be
postponed.
WED section - ELN due on Friday 6pm
THUR section - ELN due on Monday at 6pm
FRI section - ELN due on Tuesday at 6pm
PURPOSE
❏ Mention all protocols (3) protocol used!
❏ Briefly describe what was done to state WHY it was relevant!
❏ Gibson Assembly, Transformation, and Interim Lab
❏ State how these protocols will help you reach your CaMPARI project goals
*It is a given that you will be learning new lab techniques - this does NOT need to
be stated
METHODS
❏ Copy & Paste all protocols (3)
❏ Gibson Assembly, Transformation, and Interim Lab
❏ Complete the Gibson Assembly Reaction Table.
You do not need to include your plate art, but you are free to! (this does not need
to be analyzed in Results - Data or Text)
RESULTS - DATA
❏ Raw Control and Experimental plate images provided by Lab Manager Staff
❏ Include these as Panel A and Panel B (Multi-panel figure!)
❏ Remember to include appropriate annotations!
❏ Colony Counts Table - make your own or use the example in the slides!
❏ Remember to include appropriate annotations!
RESULTS - TEXT
❏ State all colony counts for each plate and which counting method was followed.
❏ Are these counts expected (do not include why or why not - this belongs in discussion).
❏ I.E.) If the Gibson Assembly went well, are these counts expected to be seen on
the experimental plate and the Control (Digested pHLsec) plate?
❏ Make a brief comparison between your control and experimental plates.
DISCUSSION
❏ What happens during the Gibson Assembly reaction?
❏ Describe the process (brief! 2-3 sentences).
❏ Why was the precise preparation for each Linear piece of DNA so necessary
(brief! 1-2 sentences) to Gibson Assembly prep.
