Portage Learning BIOD171 Microbiology Lab Exams 1-9
q q q q q q
Answer q the q following q questions
1. What q three q elements q are q used q in q an q autoclave q to q sterilize q equipment?
heat, qpressure, qand qsteam
2. What qis qthe qminimum qtemperature qan qautoclave qmust qbe qset qat qto qachieve
qsterile q condition?
125°C
3. If qyou qare qworking qin qa qlab qin qwhich qan qautoclave qis qnot qavailable, qand
you qare qpressed qfor qtime,which qwould qyou qchose qto qbest qsterilize qyour
q
equipment? qHot qsteam qor qhot qair? qExplain qwhy qyou qchose qyour qanswer.
q
Hot qsteam qis qthe qbest qchoice qas qyou qcan qachieve qa qsterile qenvironment qin qa
matter qof qminuteswhereas qhot qair qwill qtake qseveral qhours qto qachieve qthe
q
q same qeffect.
4. What qtype qof qincubator qis qpictured qbelow?
Fixed qincubator
, Answer qKey
Answer qthe qfollowing qquestions.
1. At qwhat qtemperature qis qthe qfixed qincubator qset qto, qas qpresented qin qthe qlab qvideo?
37°C
2. At qwhat qtemperature qshould qyou qrefrigerate qbacterial qsamples? qExplain qwhy qthis qis qbeneficial.
4°C. qThis qtemperature qslows qbacterial qgrowth qand qprolongs qthe qlife qof qthe qsample.
3. What qare qthe qFOUR qtypes qof qgloves qpresented qin qthe qlab qvideo?
Latex, qNitrile, qThermal qcold, qThermal qheat
4. What qTHREE qrules qwere qdiscussed qin qregards qto qlab qsafety qthat qwould qprotect qyou qand
others qfrom qcontamination?
q
1. Never qeat qor qdrink qin qthe qlab
2. Always qwear qappropriate qPPE
3. Never qleave qthe qlab qwearing qPPE
5. What qare qthe qmain qsections qthat qshould qbe qfound qin qa qlab qnotebook? qName qat qleast q4.
Objective, qProcedure, qNotes, qResults qand qDeviations
, Answer qthe qfollowing qquestions.
1. You qare qa qlab qinstructor qand qSally qMiller qhas qturned qin qher qlab qnotebook qfor
you qto qgrade qthe qlab qexperiment q#1 qon qlab qsafety. qBased qupon qwhat qwas
q
q covered qin qthe qlab qvideo, qhow qshould qSally qhaveentitled qher qexperiment?
SM01 q Lab q Safety
2. You q arrive q to q your q first q day q of q work q at q a q new qlab. qYou q are q taking qover q for
q someone qwho q took q a q newjob q at q another q lab. qYour q boss q informs q you q that
q because qof q time qrestraints, qthis qperson qdid qnot qexactlyfollow qthe qexperimental
protocol. qIn qorder qto q proceed, q you q must q know q what q he q did q differently. q (1)
q
q According q to q the q lab q lecture, qunder qwhat qsection qof qhis qnotebook q would qyou qlook
to qfind qthe qexperimental qsteps? q(2)
q q As qchanges qto qthe qexperimental qsteps qwere
made, qwhat qare qthesedifferences qcalled qand
q q how qshould qit qappear qin qthe qlab
notebook?
q
(1) Procedure—this qis qwhere qthe qsteps qfor qthe qexperimental qprotocol qare qrecorded.
(2) Deviations. qAll qdeviations qshould qbe qwritten qin qred qto qimmediately
q bring qattention qto qthechanges qin qthe qprotocol.
LAB q 2
1. Identify q the q part q of q the q microscope q indicated q by q the q arrow.
Oculars q(or qeyepiece)
q q q q q q
Answer q the q following q questions
1. What q three q elements q are q used q in q an q autoclave q to q sterilize q equipment?
heat, qpressure, qand qsteam
2. What qis qthe qminimum qtemperature qan qautoclave qmust qbe qset qat qto qachieve
qsterile q condition?
125°C
3. If qyou qare qworking qin qa qlab qin qwhich qan qautoclave qis qnot qavailable, qand
you qare qpressed qfor qtime,which qwould qyou qchose qto qbest qsterilize qyour
q
equipment? qHot qsteam qor qhot qair? qExplain qwhy qyou qchose qyour qanswer.
q
Hot qsteam qis qthe qbest qchoice qas qyou qcan qachieve qa qsterile qenvironment qin qa
matter qof qminuteswhereas qhot qair qwill qtake qseveral qhours qto qachieve qthe
q
q same qeffect.
4. What qtype qof qincubator qis qpictured qbelow?
Fixed qincubator
, Answer qKey
Answer qthe qfollowing qquestions.
1. At qwhat qtemperature qis qthe qfixed qincubator qset qto, qas qpresented qin qthe qlab qvideo?
37°C
2. At qwhat qtemperature qshould qyou qrefrigerate qbacterial qsamples? qExplain qwhy qthis qis qbeneficial.
4°C. qThis qtemperature qslows qbacterial qgrowth qand qprolongs qthe qlife qof qthe qsample.
3. What qare qthe qFOUR qtypes qof qgloves qpresented qin qthe qlab qvideo?
Latex, qNitrile, qThermal qcold, qThermal qheat
4. What qTHREE qrules qwere qdiscussed qin qregards qto qlab qsafety qthat qwould qprotect qyou qand
others qfrom qcontamination?
q
1. Never qeat qor qdrink qin qthe qlab
2. Always qwear qappropriate qPPE
3. Never qleave qthe qlab qwearing qPPE
5. What qare qthe qmain qsections qthat qshould qbe qfound qin qa qlab qnotebook? qName qat qleast q4.
Objective, qProcedure, qNotes, qResults qand qDeviations
, Answer qthe qfollowing qquestions.
1. You qare qa qlab qinstructor qand qSally qMiller qhas qturned qin qher qlab qnotebook qfor
you qto qgrade qthe qlab qexperiment q#1 qon qlab qsafety. qBased qupon qwhat qwas
q
q covered qin qthe qlab qvideo, qhow qshould qSally qhaveentitled qher qexperiment?
SM01 q Lab q Safety
2. You q arrive q to q your q first q day q of q work q at q a q new qlab. qYou q are q taking qover q for
q someone qwho q took q a q newjob q at q another q lab. qYour q boss q informs q you q that
q because qof q time qrestraints, qthis qperson qdid qnot qexactlyfollow qthe qexperimental
protocol. qIn qorder qto q proceed, q you q must q know q what q he q did q differently. q (1)
q
q According q to q the q lab q lecture, qunder qwhat qsection qof qhis qnotebook q would qyou qlook
to qfind qthe qexperimental qsteps? q(2)
q q As qchanges qto qthe qexperimental qsteps qwere
made, qwhat qare qthesedifferences qcalled qand
q q how qshould qit qappear qin qthe qlab
notebook?
q
(1) Procedure—this qis qwhere qthe qsteps qfor qthe qexperimental qprotocol qare qrecorded.
(2) Deviations. qAll qdeviations qshould qbe qwritten qin qred qto qimmediately
q bring qattention qto qthechanges qin qthe qprotocol.
LAB q 2
1. Identify q the q part q of q the q microscope q indicated q by q the q arrow.
Oculars q(or qeyepiece)
