MICROBIOLOGY LAB FINAL EXAM
QUESTIONS AND ANSWERS 100% PASS
Which staining technique is used to determine the thickness of the
peptidoglycan layer based on the cell wall composition? - ✔✔Gram stain
Where would you properly dispose of the following items? -
✔✔Contaminated slides: Slide trays on student benches
Pipettes: Nalgene buckets on student benches
Used test tubes: Trash cart at front of lab
Uncontaminated broken glass: Broken glass box at front of lab (and notify
the TA!)
When are open toed-shoes and shorts allowed in the microbiology labs? -
✔✔Never
1
100% Pass Guarantee Katelyn Whitman, All Rights
,What do you use to clean the ocular lenses and objective lenses on your
microscope? - ✔✔Lens cleaner and lens paper
________________________ microscopy is used to view dead, stained
microorganisms, and ________________________ microscopy is used to
view live, unstained microorganisms - ✔✔Brightfield, phase-contrast
What is the purpose of isolation streaks on your environmental isolate? -
✔✔To purify one species of bacteria
In microscopy, what are the three main factors to obtain a good image? -
✔✔Magnification, resolution, and contrast
Which objective lens is used with immersion oil? - ✔✔100X objective lens
When you first enter the lab and again before you leave the lab, what are
two things that you should always do? - ✔✔Wash your hands and wipe
down the lab bench with QT solution
In Exercise 1 Part 2 Phase-Contrast Microscopy, ______________
______________ was used to visualize bacteria at 1000x total magnification
with oil immersion. - ✔✔Bacillus megaterium
2
100% Pass Guarantee Katelyn Whitman, All Rights
, Which of the following is a growth streak? - ✔✔perfect up down streak
______________ ______________ is the positive control organism for the
capsule stain. - ✔✔Klebsiella pneumoniae
With regard to staining techniques used in this lab, what is the definition of
a control organism? - ✔✔A control organism is known to have a positive or
negative result for a specific staining technique and is used as a
comparison to determine the staining result of an unknown bacteria
When differentially staining bacterial cells, what is the purpose of the
steaming step? - ✔✔Endospore stain: Drives the primary stain into keratin
proteins
Capsule stain: The steaming step is not used in this staining technique
Gram stain: The steaming step is not used in this staining technique
Based on the following image, where is the appropriate place to label a
Petri plate? - ✔✔Along the side of the bottom plate
3
100% Pass Guarantee Katelyn Whitman, All Rights
QUESTIONS AND ANSWERS 100% PASS
Which staining technique is used to determine the thickness of the
peptidoglycan layer based on the cell wall composition? - ✔✔Gram stain
Where would you properly dispose of the following items? -
✔✔Contaminated slides: Slide trays on student benches
Pipettes: Nalgene buckets on student benches
Used test tubes: Trash cart at front of lab
Uncontaminated broken glass: Broken glass box at front of lab (and notify
the TA!)
When are open toed-shoes and shorts allowed in the microbiology labs? -
✔✔Never
1
100% Pass Guarantee Katelyn Whitman, All Rights
,What do you use to clean the ocular lenses and objective lenses on your
microscope? - ✔✔Lens cleaner and lens paper
________________________ microscopy is used to view dead, stained
microorganisms, and ________________________ microscopy is used to
view live, unstained microorganisms - ✔✔Brightfield, phase-contrast
What is the purpose of isolation streaks on your environmental isolate? -
✔✔To purify one species of bacteria
In microscopy, what are the three main factors to obtain a good image? -
✔✔Magnification, resolution, and contrast
Which objective lens is used with immersion oil? - ✔✔100X objective lens
When you first enter the lab and again before you leave the lab, what are
two things that you should always do? - ✔✔Wash your hands and wipe
down the lab bench with QT solution
In Exercise 1 Part 2 Phase-Contrast Microscopy, ______________
______________ was used to visualize bacteria at 1000x total magnification
with oil immersion. - ✔✔Bacillus megaterium
2
100% Pass Guarantee Katelyn Whitman, All Rights
, Which of the following is a growth streak? - ✔✔perfect up down streak
______________ ______________ is the positive control organism for the
capsule stain. - ✔✔Klebsiella pneumoniae
With regard to staining techniques used in this lab, what is the definition of
a control organism? - ✔✔A control organism is known to have a positive or
negative result for a specific staining technique and is used as a
comparison to determine the staining result of an unknown bacteria
When differentially staining bacterial cells, what is the purpose of the
steaming step? - ✔✔Endospore stain: Drives the primary stain into keratin
proteins
Capsule stain: The steaming step is not used in this staining technique
Gram stain: The steaming step is not used in this staining technique
Based on the following image, where is the appropriate place to label a
Petri plate? - ✔✔Along the side of the bottom plate
3
100% Pass Guarantee Katelyn Whitman, All Rights
