TCU Microbiology Lab Exam 1
Questions and Answers 100% Pass
chemically defined (synthetic) media - ✔✔media composed of precisely
defined chemicals
elements that all bacteria need - ✔✔carbon, nitrogen, phosphorus, sulfur,
oxygen, and hydrogen
minimal media - ✔✔media that contains exact nutrients needed for growth
and nothing extra
- media is useful b/c we know exactly what's in it, but few organisms are
resourceful enough to survive with only a few salts and sugars
complex media - ✔✔- it contains all the nutrients necessary for the
microbes
1
100% Pass Guarantee Katelyn Whitman, All Rights
,- aka general purpose media, satisfies the nutritional requirements of
bacteria with more stringent growth requirements
- consists of mix of digests & extracts from plant or animal tissue
- nutrient rich but poorly defined with the exact nutrients unknown
- in lab we use complex media: tryptic soy media
enriched media - ✔✔complex media that contains additional "special"
nutrients/additives that are not commonly found in media
- least commonly used media
autoclave - ✔✔media is subjected to high-pressure steam at 121C at 15 lbs.
of pressure to sterilize and destroy any contaminants present
two major forms of media - ✔✔liquid and solid media
- media solidifies by adding agar (1.5%)
- agar is inert and incapable of growing by itself
what temperature do most bacterial organisms grow at? - ✔✔37C (98.6F)
2
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, colony - ✔✔A population of cells arising from a bacterial cell group or from
a group of attached bacterial cells
colony forming units (cfu) - ✔✔estimate the approximate number of
bacteria in a liquid broth culture
simple microscope - ✔✔contains a single magnifying lens and is similar to
a magnifying lens
compound microscope - ✔✔consists of two singles lenses arranged one in
front of the other so that the second lens magnifies the image produced by
the first
illuminator - ✔✔light emits from this
condenser - ✔✔light passes through this and concentrates the light and
focuses the light onto the specimen
parfocal - ✔✔property of a microscope which allows objectives to be
changed with minimal or no refocusing
total magnification - ✔✔objective magnification (4X, 10X, 40X, 100X) x
ocular magnification (10X)
3
100% Pass Guarantee Katelyn Whitman, All Rights
Questions and Answers 100% Pass
chemically defined (synthetic) media - ✔✔media composed of precisely
defined chemicals
elements that all bacteria need - ✔✔carbon, nitrogen, phosphorus, sulfur,
oxygen, and hydrogen
minimal media - ✔✔media that contains exact nutrients needed for growth
and nothing extra
- media is useful b/c we know exactly what's in it, but few organisms are
resourceful enough to survive with only a few salts and sugars
complex media - ✔✔- it contains all the nutrients necessary for the
microbes
1
100% Pass Guarantee Katelyn Whitman, All Rights
,- aka general purpose media, satisfies the nutritional requirements of
bacteria with more stringent growth requirements
- consists of mix of digests & extracts from plant or animal tissue
- nutrient rich but poorly defined with the exact nutrients unknown
- in lab we use complex media: tryptic soy media
enriched media - ✔✔complex media that contains additional "special"
nutrients/additives that are not commonly found in media
- least commonly used media
autoclave - ✔✔media is subjected to high-pressure steam at 121C at 15 lbs.
of pressure to sterilize and destroy any contaminants present
two major forms of media - ✔✔liquid and solid media
- media solidifies by adding agar (1.5%)
- agar is inert and incapable of growing by itself
what temperature do most bacterial organisms grow at? - ✔✔37C (98.6F)
2
100% Pass Guarantee Katelyn Whitman, All Rights
, colony - ✔✔A population of cells arising from a bacterial cell group or from
a group of attached bacterial cells
colony forming units (cfu) - ✔✔estimate the approximate number of
bacteria in a liquid broth culture
simple microscope - ✔✔contains a single magnifying lens and is similar to
a magnifying lens
compound microscope - ✔✔consists of two singles lenses arranged one in
front of the other so that the second lens magnifies the image produced by
the first
illuminator - ✔✔light emits from this
condenser - ✔✔light passes through this and concentrates the light and
focuses the light onto the specimen
parfocal - ✔✔property of a microscope which allows objectives to be
changed with minimal or no refocusing
total magnification - ✔✔objective magnification (4X, 10X, 40X, 100X) x
ocular magnification (10X)
3
100% Pass Guarantee Katelyn Whitman, All Rights
