Microbiology Lab Practical 1 (TAMUCC) Exam Questions and Answers 100% Pass

Microbiology Lab Practical 1 (TAMUCC) Exam Questions and Answers 100% Pass How is total magnification calculated? - Multiply the ocular lens (10x) by the objective lens 10x*10=100x 40x*10=400x 100x*10=1000x OCULAR TIME OBJECTIVE what is the function of immersion oil when using the oil immersion objective? - the immersion oil allows for magnification and increases the contrast of the specimen 2 100% Pass Guarantee Katelyn Whitman, All Rights What is meant by the term parfocal? - Once the lens is focused on a particular area it will remain focused on the particular area regardless of changing the objective why aren't the magnifications of both lenses of binocular microscope used to calculate total magnification? - Because the image only goes through one ocular to reach both eyes Why is the 10x placed in position when the microscope is stored or carried? - The low-power objective is farther away from the stage than the other objectives so the lens is less likely to get scrapped during handling. What is an aseptic technique? What is its importance? Why do you always have to do it in the microbiology lab? - -Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. -Proper aseptic technique prevents contamination of cultures from foreign bacteria inherent in the environment. 3 100% Pass Guarantee Katelyn Whitman, All Rights what are some aseptic techniques used when transfering cultures from plate to broth tube and agar slant? - 1. Prepare your desktop by swabbing down its surface with a disinfectant. 2. Wash your hands 3. With a marking pen, label a tube of sterile nutrient broth with name, date and specimen 4. Sterilize the inoculating loop by holding it over the flame of a Bunsen burner until it becomes bright red. The entire wire must be heated. what is a culture medium? - An artificial environment that provides water and nutrients for the microorganism