Microbiology Lab Final Exam Questions and Answers 100% Pass

Microbiology Lab Final Exam Questions and Answers 100% Pass Give the steps of a PCR - Heat reaction to 95 C to denature DNA Cool rxn to 55 C to allow primers to anneal Heat rxn to 72 C to allow Taq polymerase to bind What machine is used to carry out a PCR reaction? - thermocycler How many cycles does a PCR rxn usually go? - 30 Name the 9 organisms used in the unknown lab - Bacillus subtillis Enterobacter aerogenes Entero cloacae Escherichia coli Klebsiella pneumoniae 2 100% Pass Guarantee Katelyn Whitman, All Rights Pseudomonas aeruginosa Salmonella typhimurium Staphylococcus aureus Staphylococcus epidermidis Which organisms can ferment lactose? - E. coli E. cloacae E. aerogenes K. pneumonia Define caliform bacteria - rod-shaped, Gram-negative, non-spore forming, and motile or non-motile bacteria which can ferment lactose with the production of acid and gas Name the selective medias used in class - Blood Agar Bile Esculin Mannitol Salt Agar (MSA) MacConkey Agar (MaC) 3 100% Pass Guarantee Katelyn Whitman, All Rights Eosin Methylene Blue (EMB) Hektoen Enteric Agar (HEA) Xylose Lysine Desoxiolate (XLD) Name the differential medias used in class - O/F Glucose MRVP Catalase Oxidase Citrate Starch Indole TSI What does the acronym IMViC stand for? - indol