Microbio 150 Lab exam 1 PVCC
Questions and Answers 100% Pass
When preparing to view a specimen on a compound microscope in the lab,
one should __________ begin with the 4x objective - ✔✔Always
Where should used gloves be disposed of in the microbiology lab - ✔✔In
the bio-hazard waste container
Before putting a dust cover over the microscope and placing it in the
cabinet, which objective lens should be rotated over the stage - ✔✔4x
On the microscope, what concentrates the light on the specimen? -
✔✔Condenser lens
On the microscope, what bends light passing through the specimen to
create a real image - ✔✔Objective lens
1
100% Pass Guarantee Katelyn Whitman, All Rights
, On the microscope, what allows for more or less light to be directed onto
the specimen - ✔✔Iris diaphragm
On the microscope, what gives you clarity of the image - ✔✔Resolution
Why is desiccating your inoculating loop important? When should this be
done? - ✔✔To ensure it is sterilized, done so no cross contamination
happens
During an aseptic transfer, when do you pass the mouth of the test tube
through the flame? - ✔✔Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain
a pure culture from a __________. - ✔✔Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to -
✔✔Attach it firmly to the slide
The term that refers to the purposeful addition of microorganisms into a
nutrient medium is - ✔✔Inoculation
True or false: In the microbiology lab, it is acceptable to move your loop
around while waiting for it to dry. - ✔✔False
2
100% Pass Guarantee Katelyn Whitman, All Rights
Questions and Answers 100% Pass
When preparing to view a specimen on a compound microscope in the lab,
one should __________ begin with the 4x objective - ✔✔Always
Where should used gloves be disposed of in the microbiology lab - ✔✔In
the bio-hazard waste container
Before putting a dust cover over the microscope and placing it in the
cabinet, which objective lens should be rotated over the stage - ✔✔4x
On the microscope, what concentrates the light on the specimen? -
✔✔Condenser lens
On the microscope, what bends light passing through the specimen to
create a real image - ✔✔Objective lens
1
100% Pass Guarantee Katelyn Whitman, All Rights
, On the microscope, what allows for more or less light to be directed onto
the specimen - ✔✔Iris diaphragm
On the microscope, what gives you clarity of the image - ✔✔Resolution
Why is desiccating your inoculating loop important? When should this be
done? - ✔✔To ensure it is sterilized, done so no cross contamination
happens
During an aseptic transfer, when do you pass the mouth of the test tube
through the flame? - ✔✔Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain
a pure culture from a __________. - ✔✔Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to -
✔✔Attach it firmly to the slide
The term that refers to the purposeful addition of microorganisms into a
nutrient medium is - ✔✔Inoculation
True or false: In the microbiology lab, it is acceptable to move your loop
around while waiting for it to dry. - ✔✔False
2
100% Pass Guarantee Katelyn Whitman, All Rights
