Microbiology Lab Exam 1 Notes
Questions and Answers 100% Pass
when viewing a smear with the oil immersion lens the final magnification
is - ✔✔1000x;10x ocular x 100X objective
what is the purpose of heat fixing - ✔✔kill the bacteria, adhere to glass
anddenature the proteins of bacteria
name oneof the stains or regents used in the gram stain procedure -
✔✔gram crystal violet, gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - ✔✔to ensure that we do not
contaminate what we are working with as as to ensue that we do not
acciently inoculate ouselves. example of aseptic techniques are flaming the
loop/needle sterilizing it, breifly flaming the test tube before and after
obtaining the sample.
1
100% Pass Guarantee Katelyn Whitman, All Rights
, the gram reaction is due to differences in the structure of wat part of the
microbe - ✔✔its cell wall, specifically peptidoglycan, gram positive bacteria
have thicker peptidoglycan layer compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab - ✔✔loop, needle
what color is a gram positive microbe - ✔✔purple
waht is the function of the ethanol in the gram stain procedure -
✔✔decolorize
name one of the strains in the acid fast stain - ✔✔cold kinyoun, carbol
fuschsin, methylen blue
name one of the stains in the endospore stain - ✔✔malachite green,
safranin
what is the purpose of the acid-fast stain - ✔✔the purpose of the acid-fast
stain is to differentiate between the myobactriace family of bacteria from all
other bacteria
what color does na acid-fast microbe stain with acd-fast stain - ✔✔red
2
100% Pass Guarantee Katelyn Whitman, All Rights
Questions and Answers 100% Pass
when viewing a smear with the oil immersion lens the final magnification
is - ✔✔1000x;10x ocular x 100X objective
what is the purpose of heat fixing - ✔✔kill the bacteria, adhere to glass
anddenature the proteins of bacteria
name oneof the stains or regents used in the gram stain procedure -
✔✔gram crystal violet, gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - ✔✔to ensure that we do not
contaminate what we are working with as as to ensue that we do not
acciently inoculate ouselves. example of aseptic techniques are flaming the
loop/needle sterilizing it, breifly flaming the test tube before and after
obtaining the sample.
1
100% Pass Guarantee Katelyn Whitman, All Rights
, the gram reaction is due to differences in the structure of wat part of the
microbe - ✔✔its cell wall, specifically peptidoglycan, gram positive bacteria
have thicker peptidoglycan layer compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab - ✔✔loop, needle
what color is a gram positive microbe - ✔✔purple
waht is the function of the ethanol in the gram stain procedure -
✔✔decolorize
name one of the strains in the acid fast stain - ✔✔cold kinyoun, carbol
fuschsin, methylen blue
name one of the stains in the endospore stain - ✔✔malachite green,
safranin
what is the purpose of the acid-fast stain - ✔✔the purpose of the acid-fast
stain is to differentiate between the myobactriace family of bacteria from all
other bacteria
what color does na acid-fast microbe stain with acd-fast stain - ✔✔red
2
100% Pass Guarantee Katelyn Whitman, All Rights
