MCB 4403 exam 1 review (updated)
questions well answered
A 2-D protein gel separates proteins based on two properties. In the 2nd dimension, the
separation is based on mass/size. What is usually the basis of the separation in the 1st
dimension? - correct answer ✔✔isoelectric point (pI)
A 0.2 micrometer (micron) filter is used to filter-sterilize growth medium that will be used to
grow a human cell line. Which of these microbes would NOT be trapped and removed by the
filter?
0. E.coli
1. Plamodium falciparum
2. poliovirus
3. Streptococcus pneumoniae
4. all
5. none - correct answer ✔✔2. poliovirus
If you look through a standard microscope and all you see is a blinding light, you would adjust
the ________. If the image looks blurry, you would FIRST try to adjust the focus of
the_________ - correct answer ✔✔diaphragm, objective lens
Mycobacterium tuberculosis would appear ________ when visualized on a light microscope
after all the steps of the GRAM STAIN procedure - correct answer ✔✔colorless
Which of these techniques would be the best choice(s) for observing FINE DETAILS of structures
on the SURFACE of a bacterial cell, such as fimbriae and pili? - correct answer ✔✔scanning
electron microscopy
atomic force microscopy
, Which of these antibiotics interferes with function of bacterial topiosomerase, preventing
proper genome maintenance? - correct answer ✔✔quinolones
Most strains of yeast can grow in a flask with vigorous shaking/aeration and can also grow in a
completely sealed wine barrel. Yeast is a - correct answer ✔✔facultative aerobe/anaerobe
N-acetylglucosamine and N-acetylmuramic acid are the major components of - correct answer
✔✔peptidoglycan
Viruses are replicating their genome, making proteins, and assembling virions during the
_________ period - correct answer ✔✔eclipse
Phosphotransferase systems use energy from __________ to bring sugars such as glucose into
the cell. - correct answer ✔✔phosphoenol-pyruvate (PEP)
Which of these antibiotics interferes with bacterial translation? - correct answer
✔✔streptomycin
the cell wall of Gram-negative bacteria is located in the structure called the - correct answer
✔✔periplasm
Which of these proteins guides bacterial RNA polymerase to the DNA promoter sequence? -
correct answer ✔✔sigma factor
A difference between dideoxyribonucleotides (ddNTPs) and deoxyribonucleotides (dNTPs) is
that ddNTPs are missing an -OH group on carbon ________ of the sugar - correct answer ✔✔3
questions well answered
A 2-D protein gel separates proteins based on two properties. In the 2nd dimension, the
separation is based on mass/size. What is usually the basis of the separation in the 1st
dimension? - correct answer ✔✔isoelectric point (pI)
A 0.2 micrometer (micron) filter is used to filter-sterilize growth medium that will be used to
grow a human cell line. Which of these microbes would NOT be trapped and removed by the
filter?
0. E.coli
1. Plamodium falciparum
2. poliovirus
3. Streptococcus pneumoniae
4. all
5. none - correct answer ✔✔2. poliovirus
If you look through a standard microscope and all you see is a blinding light, you would adjust
the ________. If the image looks blurry, you would FIRST try to adjust the focus of
the_________ - correct answer ✔✔diaphragm, objective lens
Mycobacterium tuberculosis would appear ________ when visualized on a light microscope
after all the steps of the GRAM STAIN procedure - correct answer ✔✔colorless
Which of these techniques would be the best choice(s) for observing FINE DETAILS of structures
on the SURFACE of a bacterial cell, such as fimbriae and pili? - correct answer ✔✔scanning
electron microscopy
atomic force microscopy
, Which of these antibiotics interferes with function of bacterial topiosomerase, preventing
proper genome maintenance? - correct answer ✔✔quinolones
Most strains of yeast can grow in a flask with vigorous shaking/aeration and can also grow in a
completely sealed wine barrel. Yeast is a - correct answer ✔✔facultative aerobe/anaerobe
N-acetylglucosamine and N-acetylmuramic acid are the major components of - correct answer
✔✔peptidoglycan
Viruses are replicating their genome, making proteins, and assembling virions during the
_________ period - correct answer ✔✔eclipse
Phosphotransferase systems use energy from __________ to bring sugars such as glucose into
the cell. - correct answer ✔✔phosphoenol-pyruvate (PEP)
Which of these antibiotics interferes with bacterial translation? - correct answer
✔✔streptomycin
the cell wall of Gram-negative bacteria is located in the structure called the - correct answer
✔✔periplasm
Which of these proteins guides bacterial RNA polymerase to the DNA promoter sequence? -
correct answer ✔✔sigma factor
A difference between dideoxyribonucleotides (ddNTPs) and deoxyribonucleotides (dNTPs) is
that ddNTPs are missing an -OH group on carbon ________ of the sugar - correct answer ✔✔3
